Search Results - "Hedgehog Signaling"

Noncanonical Hedgehog Signaling by Brennan, Donna, Chen, Xiaole, Cheng, Lan, Mahoney, My, Riobo, Natalia A.

“…The notion of noncanonical hedgehog (Hh) signaling in mammals has started to receive support from numerous observations. By noncanonical, we refer to all those…”
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Evaluating Hedgehog Acyltransferase Activity and Inhibition Using the Acylation-coupled Lipophilic Induction of Polarization (Acyl-cLIP) Assay by Andrei, Sebastian A, Tate, Edward W, Lanyon-Hogg, Thomas

“…Palmitoylation of the Hedgehog family of proteins is a critical step in the Hedgehog signaling pathway and is performed by the membrane-bound…”
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Hedgehog Signaling by Choy, Siu Wah, Cheng, Shuk Han

“…The original hedgehog (hh) gene was found in Drosophila and named for the appearance of a mutant phenotype which causes an embryo to be covered with pointy…”
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An In Situ Fluorescence Assay for Cholesterol Transporter Activity of the Patched by Cho, Wonhwa, Ralko, Arthur, Sharma, Ashutosh

“…We recently developed a simultaneous in situ quantitative imaging technique for cholesterol in both leaflets of the plasma membrane of mammalian cells. This…”
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Generation and Analysis of Mosaic Spinal Cord Organoids Derived from Mouse Embryonic Stem Cells by Jägers, Carina, Roelink, Henk

“…Three central cell populations play roles in morphogen action, the cells that produce, the cells that distribute, and the cells that respond to the morphogen…”
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The Immunofluorescence-Based Detection of Hedgehog Pathway Components in Primary Cilia of Cultured Cells by Yang, Shu, Zheng, Xiaoyan

“…The primary cilium is a microtubule-based organelle that projects from the surface of vertebrate cells. Defects in the biogenesis of or transport through…”
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Characterization of Smoothened Phosphorylation and Activation by Jia, Jianhang, Han, Yuhong, Cho, Yong Suk, Jiang, Jin

“…The GPCR-family protein Smoothened (Smo) is an obligatory signal transducer of the Hedgehog (Hh) signaling pathway. Binding of Hh to its receptor Patched (Ptc)…”
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Imaging of the Ciliary Cholesterol Underlying the Sonic Hedgehog Signal Transduction by Miyamoto, Tatsuo, Hosoba, Kosuke, Akutsu, Silvia Natsuko, Matsuura, Shinya

“…Primary cilia are antenna-like structures that develop on the surface of quiescent G -phase cells and receive extracellular signals including sonic hedgehog…”
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Studies of SMOOTHENED Activation in Cell-Free and Reconstituted Systems by Nelson, Isaac B, Myers, Benjamin R

“…Much of our current understanding of Hedgehog signal transduction derives from studies involving intact cells and organisms. Here we describe the use of…”
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Expression, Purification, and Structure Determination of Human PTCH1-HH-N Complexes by Qi, Xiaofeng, Schmiege, Philip, Esparza, Leticia, Li, Xiaochun

“…Patched-1 (PTCH1), a tumor suppressor, serves as the receptor of Hedgehog (HH) ligand and negatively regulates the HH signaling pathway. Mutations of PTCH1 are…”
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Analysis of Protein Cholesterylation by Biorthogonal Labeling by Hu, Ao, Zhou, Meng, Song, Bao-Liang

“…Cholesterol modification (or cholesterylation) is a rare but important posttranslational lipid modification of proteins. So far, two proteins, namely Hedgehog…”
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Rapid, Direct SMOOTHENED Activity Assays in Live Cells Using cAMP-Based Conformational Sensors by Walker, Madison F, Myers, Benjamin R

“…Communication between PATCHED1 (PTCH1) and SMOOTHENED (SMO) is fundamental to Hedgehog (Hh) signal transduction in development and disease. We describe a…”
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Light-Induced Differentiation of Forebrain Organoids by NVOC-SAG by Misawa, Ryuji, Ikeuchi, Yoshiho

“…Hedgehog signaling pathway shapes our body by regulating proliferation and differentiation of cells. The spatial and temporal distribution pattern of its…”
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Studying Hedgehog Signaling During Mouse Neural Tube Development by Constable, Sandii, White, Kevin, Somatilaka, Bandarigoda Nipunika, Mukhopadhyay, Saikat

“…The identity of ventral neural progenitors in the neural tube is largely dependent on Hedgehog (Hh) signaling. Variations in staining patterns are…”
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A Method for Orthotopic Transplantation of Lung Cancer in Mice by Wilson, Alexandra N, Chen, Baozhi, Liu, Xin, Kurie, Jonathan M, Kim, James

“…Preclinical mouse models of lung cancer have been vital experimental tools to elucidate cancer biology and test novel therapeutic regimens. Two main models are…”
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Cell-Based Assays for Smoothened Ubiquitination and Sumoylation by Han, Yuhong, Jiang, Jin

“…The Hedgehog (Hh) family of secreted proteins governs embryonic development and adult tissue homeostasis by regulating the abundance, localization, and…”
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Measuring and Manipulating Membrane Cholesterol for the Study of Hedgehog Signaling by Kinnebrew, Maia, Johnson, Kristen A, Radhakrishnan, Arun, Rohatgi, Rajat

“…Cholesterol is an abundant lipid in mammalian plasma membranes that regulates the reception of the Hedgehog (Hh) signal in target cells. In vertebrates,…”
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Ci/Gli Phosphorylation by the Fused/Ulk Family Kinases by Han, Yuhong, Jiang, Jin

“…Hedgehog (Hh) signaling culminates in the conversion of the latent transcription factor Cubitus interruptus (Ci)/Gli from a repressor form (Ci /Gli ) into an…”
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Analysis of Dispatched Protein Processing and Sonic Hedgehog Ligand Release by Cleverdon, Elizabeth, Stewart, Daniel P, Ogden, Stacey K

“…The 12-pass transmembrane protein Dispatched (DISP) is essential for Sonic Hedgehog (SHH) release from ligand-producing cells and is indispensable for…”
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Biochemical Assays to Directly Assess Smoothened Activation by a Conformationally Sensitive Nanobody by Deshpande, Ishan, Manglik, Aashish

“…The Hedgehog signaling pathway coordinates early development and is important in various cancers. Classic approaches to test pathway activation rely on…”
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