Search Results - "Heck, J"

High-Resolution Native Mass Spectrometry by Tamara, Sem, den Boer, Maurits A, Heck, Albert J. R

“…Native mass spectrometry (MS) involves the analysis and characterization of macromolecules, predominantly intact proteins and protein complexes, whereby as…”
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Proteomics beyond trypsin by Tsiatsiani, Liana, Heck, Albert J. R

“…Peptide‐centered shotgun analysis of proteins has been the core technology in mass spectrometry based proteomics and has enabled numerous biological…”
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Native Mass Spectrometry: What is in the Name? by Leney, Aneika C., Heck, Albert J. R.

“…Electrospray ionization mass spectrometry (ESI-MS) is nowadays one of the cornerstones of biomolecular mass spectrometry and proteomics. Advances in sample…”
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Microtubule minus-end regulation at spindle poles by an ASPM–katanin complex by Jiang, Kai, Rezabkova, Lenka, Hua, Shasha, Liu, Qingyang, Capitani, Guido, Altelaar, A. F. Maarten, Heck, Albert J. R., Kammerer, Richard A., Steinmetz, Michel O., Akhmanova, Anna

“…ASPM (known as Asp in fly and ASPM-1 in worm) is a microcephaly-associated protein family that regulates spindle architecture, but the underlying mechanism is…”
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Resolving heterogeneous macromolecular assemblies by Orbitrap-based single-particle charge detection mass spectrometry by Wörner, Tobias P., Snijder, Joost, Bennett, Antonette, Agbandje-McKenna, Mavis, Makarov, Alexander A., Heck, Albert J. R.

“…We demonstrate single-particle charge detection mass spectrometry on an Orbitrap for the analysis of megadalton biomolecular assemblies. We establish that the…”
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Adeno-associated virus capsid assembly is divergent and stochastic by Wörner, Tobias P., Bennett, Antonette, Habka, Sana, Snijder, Joost, Friese, Olga, Powers, Thomas, Agbandje-McKenna, Mavis, Heck, Albert J. R.

“…Adeno-associated viruses (AAVs) are increasingly used as gene therapy vectors. AAVs package their genome in a non-enveloped T  = 1 icosahedral capsid of ~3.8…”
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Six alternative proteases for mass spectrometry–based proteomics beyond trypsin by Giansanti, Piero, Tsiatsiani, Liana, Low, Teck Yew, Heck, Albert J R

“…The use of a single enzyme such as trypsin for shotgun proteomics limits the ability to cover the whole proteome and all protein post-translational…”
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Identification of CMTM6 and CMTM4 as PD-L1 protein regulators by Mezzadra, Riccardo, Sun, Chong, Jae, Lucas T., Gomez-Eerland, Raquel, de Vries, Evert, Wu, Wei, Logtenberg, Meike E. W., Slagter, Maarten, Rozeman, Elisa A., Hofland, Ingrid, Broeks, Annegien, Horlings, Hugo M., Wessels, Lodewyk F. A., Blank, Christian U., Xiao, Yanling, Heck, Albert J. R., Borst, Jannie, Brummelkamp, Thijn R., Schumacher, Ton N. M.

“…CMTM6 and CMTM4 bind to and stabilize the inhibitory receptor PD-L1 and regulate PD-L1 levels at the surface of human tumour and immune cells. Regulating…”
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Efficient and robust proteome-wide approaches for cross-linking mass spectrometry by Klykov, Oleg, Steigenberger, Barbara, Pektaş, Sibel, Fasci, Domenico, Heck, Albert J. R., Scheltema, Richard A.

“…Cross-linking mass spectrometry (XL-MS) has received considerable interest, owing to its potential to investigate protein–protein interactions (PPIs) in an…”
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Next-generation proteomics: towards an integrative view of proteome dynamics by Altelaar, A. F. Maarten, Munoz, Javier, Heck, Albert J. R.

“…Key Points Our understanding of cellular function depends on exquisite knowledge of all of the molecular components acting in a system. Mass spectrometry…”
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Optimized fragmentation schemes and data analysis strategies for proteome-wide cross-link identification by Liu, Fan, Lössl, Philip, Scheltema, Richard, Viner, Rosa, Heck, Albert J. R.

“…We describe optimized fragmentation schemes and data analysis strategies substantially enhancing the depth and accuracy in identifying protein cross-links…”
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Native mass spectrometry: a bridge between interactomics and structural biology by Heck, Albert J R

“…Native mass spectrometry is an emerging technology that allows the topological investigation of intact protein complexes with high sensitivity and a…”
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Serine 25 phosphorylation inhibits RIPK1 kinase-dependent cell death in models of infection and inflammation by Dondelinger, Yves, Delanghe, Tom, Priem, Dario, Wynosky-Dolfi, Meghan A., Sorobetea, Daniel, Rojas-Rivera, Diego, Giansanti, Piero, Roelandt, Ria, Gropengiesser, Julia, Ruckdeschel, Klaus, Savvides, Savvas N., Heck, Albert J. R., Vandenabeele, Peter, Brodsky, Igor E., Bertrand, Mathieu J. M.

“…RIPK1 regulates cell death and inflammation through kinase-dependent and -independent mechanisms. As a scaffold, RIPK1 inhibits caspase-8-dependent apoptosis…”
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A large fraction of HLA class I ligands are proteasome-generated spliced peptides by Liepe, Juliane, Marino, Fabio, Sidney, John, Jeko, Anita, Bunting, Daniel E., Sette, Alessandro, Kloetzel, Peter M., Stumpf, Michael P. H., Heck, Albert J. R., Mishto, Michele

“…The proteasome generates the epitopes presented on human leukocyte antigen (HLA) class I molecules that elicit CD8⁺ T cell responses. Reports of…”
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Toward a Comprehensive Characterization of a Human Cancer Cell Phosphoproteome by Zhou, Houjiang, Di Palma, Serena, Preisinger, Christian, Peng, Mao, Polat, Ayse Nur, Heck, Albert J. R, Mohammed, Shabaz

“…Mass spectrometry (MS)-based phosphoproteomics has achieved extraordinary success in qualitative and quantitative analysis of cellular protein phosphorylation…”
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Seasonal variation in the Dutch bovine raw milk composition by Heck, J M L, van Valenberg, H J F, Dijkstra, J, van Hooijdonk, A C M

“…In this study, we determined the detailed composition of and seasonal variation in Dutch dairy milk. Raw milk samples representative of the complete Dutch milk…”
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Proteome-wide profiling of protein assemblies by cross-linking mass spectrometry by Liu, Fan, Rijkers, Dirk T S, Post, Harm, Heck, Albert J R

“…A crosslinking-mass spectrometry strategy, including a new proteome database search engine called XlinkX, enables the identification of inter- and…”
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Histidine phosphorylation in human cells; a needle or phantom in the haystack? by Leijten, Niels M., Heck, Albert J. R., Lemeer, Simone

“…It has been suggested that in mammalian cells histidine residues in proteins may become as frequently phosphorylated as serine, threonine and tyrosine, and may…”
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Defining the Stoichiometry and Cargo Load of Viral and Bacterial Nanoparticles by Orbitrap Mass Spectrometry by Snijder, Joost, van de Waterbeemd, Michiel, Damoc, Eugen, Denisov, Eduard, Grinfeld, Dmitry, Bennett, Antonette, Agbandje-McKenna, Mavis, Makarov, Alexander, Heck, Albert J. R

“…Accurate mass analysis can provide useful information on the stoichiometry and composition of protein-based particles, such as virus-like assemblies. For…”
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Complete and cooperative in vitro assembly of computationally designed self-assembling protein nanomaterials by Wargacki, Adam J., Wörner, Tobias P., van de Waterbeemd, Michiel, Ellis, Daniel, Heck, Albert J. R., King, Neil P.

“…Recent advances in computational methods have enabled the predictive design of self-assembling protein nanomaterials with atomic-level accuracy. These design…”
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