Search Results - "Haynes, C.A."

Inexpensive and Generic Affinity Purification of Recombinant Proteins Using a Family 2a CBM Fusion Tag by Rodriguez, Beatriz, Kavoosi, Mojgan, Koska, Jürgen, Creagh, A. Louise, Kilburn, Douglas G., Haynes, Charles A.

“…The selective binding of the family 2a carbohydrate binding module (CBM2a) of xylanase 10A of the soil bacterium Cellulomonas fimi to a variety of cellulosic…”
Get full text

Stabilities of intact hemoglobin molecules and hemoglobin peptides in dried blood samples by Adam, B.W., Haynes, C.A., Chafin, D.L., De Jesus, V.R.

Get full text

Structures and Stabilities of Adsorbed Proteins by Haynes, Charles A., Norde, Willem

“…Structural perturbations and thermodynamic-stability changes in two similar-sized globular proteins, hen egg-white lysozyme and bovine milk α-lactalbumin, upon…”
Get full text

Driving Forces for DNA Adsorption to Silica in Perchlorate Solutions by Melzak, Kathryn A., Sherwood, Chris S., Turner, Robin F.B., Haynes, Charles A.

“…The adsorption of both plasmid and chromosomal duplex DNA to silica is investigated with the aim of determining the dominant forces involved in the binding…”
Get full text

Performance of succinylacetone assays and their associated proficiency testing outcomes by Adam, B.W., Hall, E.M., Meredith, N.K., Lim, T.H., Haynes, C.A., De Jesus, V.R., Hannon, W.H.

“…Succinylacetone (SUAC) is the primary metabolic marker for hepatorenal tyrosinemia. We used results reported for dried-blood-spot proficiency testing (PT)…”
Get full text

Entropic interaction chromatography: separating proteins on the basis of size using end-grafted polymer brushes by Pang, P, Koska, J, Coad, B.R, Brooks, D.E, Haynes, C.A

“…Partitioning of a macromolecule into the interfacial volume occupied by a grafted polymer brush decreases the configurational entropy (deltaScbrush) of the…”
Get full text

Controlled shear affinity filtration (CSAF): A new technology for integration of cell separation and protein isolation from mammalian cell cultures by Vogel, Jens H., Anspach, Birger, Kroner, Karl-Heinz, Piret, James M., Haynes, Charles A.

“…Controlled shear affinity filtration (CSAF) integrates animal cell separation and product isolation in a single unit operation through the use of a…”
Get full text

Self-activating factor X derivative fused to the C-terminus of a cellulose-binding module: Production and properties by Kwan, Emily, Guarna, M. Marta, Boraston, Alisdair B., Gilkes, Neil R., Haynes, Charles A., Kilburn, Douglas G., Warren, R. Antony J.

“…In this work, a new derivative of FX was engineered. It comprises a cellulose‐binding module (CBM) fused to the N‐terminus of the truncated light chain (E2FX)…”
Get full text

Calcium Binding by the N-Terminal Cellulose-Binding Domain from Cellulomonas fimi β-1,4-Glucanase CenC by Johnson, Philip E, Creagh, A. Louise, Brun, Emmanuel, Joe, Koman, Tomme, Peter, Haynes, Charles A, McIntosh, Lawrence P

“…The interaction of the N-terminal cellulose-binding domain, CBDN1, from Cellulomonas fimi β-1,4-glucanase CenC with calcium was investigated using NMR…”
Get full text

Very high-level production and export in Escherichia coli of a cellulose binding domain for use in a generic secretion-affinity fusion system by Hasenwinkle, Diane, Jervis, Eric, Kops, Oliver, Liu, Chang, Lesnicki, Gary, Haynes, Charles A., Kilburn, Douglas G.

“…A novel expression vector pTugA, previously constructed in our laboratory, was modified to provide kanamycin resistance (pTugK) and used to direct the…”
Get full text

Concentration-dependent internalization of a cytokine/cytokine receptor complex in human hematopoietic cells by Zandstra, P. W., Jervis, E., Haynes, C. A., Kilburn, D. G., Eaves, C. J., Piret, J. M.

“…Soluble steel factor (SF) is a potent stimulator of hematopoietic progenitor cell proliferation in vitro, and cytokine combinations that include SF can support…”
Get full text

Intermolecular interactions' in aqueous solutions of three components including lysozyme by Koga, Yoshikata, Westh, Peter, Trandum, Christa, Haynes, Charles A.

“…Excess partial molar enthalpies were measured in ternary aqueous solutions, tert-butyl alcohol (TBA)-DMSO-H 2O, and lysozyme (L)–alcohols (A)–H 2O. The…”
Get full text

Thermodynamic properties of aqueous polymer solutions: poly(ethylene glycol)/dextran by Haynes, C. A, Beynon, R. A, King, R. S, Blanch, H. W, Prausnitz, J. M

Get full text

Analysis of brush-particle interactions using self-consistent-field theory by Steels, B.M., Koska, J., Haynes, C.A.

“…Non-specific protein adsorption can be reduced by attaching polymer chains by one end to a sorbent surface. End-grafted polymer modified surfaces have also…”
Get full text

Detection and determination of aflatoxins using affinity chromatography by Candlish, A.A.G, Haynes, C.A, Stimson, W.H

“…Summary Aflatoxins B1, B2, G1 and G2 can be easily and rapidly detected in aqueous solutions using an affinity chromatography column coupled to a monoclonal…”
Get full text

Analysis of compression of polymer mushrooms using self-consistent field theory by Steels, B.M, Leermakers, F.A.M, Haynes, C.A

“…A number of new technologies, including new-generation biomaterials and chromatography resins, are based on passivation and modification of surfaces by…”
Get full text