A role of the -35 element in the initiation of transcription at psbA promoter in tobacco plastids

Most plastid promoters recognized by bacteria-like plastid RNA polymerase (PEP) are similar to E. coli σ70-type promoters comprising “–35” and “–10” elements. Among them, psbA promoter is unique in bearing additional elements between the conserved –35 and –10 elements. The psbA promoter activity is...

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Bibliographic Details
Published in:	Plant and cell physiology Vol. 44; no. 3; pp. 334 - 341
Main Authors:	Hayashi, K. (Kyoto Univ. (Japan)), Shiina, T, Ishii, N, Iwai, K, Ishizaki, Y, Morikawa, K, Toyoshima, Y
Format:	Journal Article
Language:	English
Published:	Japan Oxford University Press 01-03-2003 Oxford Publishing Limited (England)
Subjects:	Base Sequence CHLOROPLASTS Chloroplasts - genetics DNA GENETIC TRANSFORMATION GENETIC VECTORS GFP green fluorescent protein Keywords: –35 element — Chloroplast transformation — GFP — Nicotiana tabacum — psbA promoter — Transcription Light MCS Molecular Sequence Data multiple cloning sites NEP Nicotiana - genetics NICOTIANA TABACUM nuclear-encoded plastid RNA polymerase NUCLEOTIDE SEQUENCE PEP Photosynthetic Reaction Center Complex Proteins - genetics Photosynthetic Reaction Center Complex Proteins - radiation effects Photosystem II Protein Complex Plant Leaves - genetics Plant Leaves - growth & development Plant Leaves - radiation effects Plants, Genetically Modified plastid-encoded plastid RNA polymerase PLASTIDS Promoter Regions, Genetic - genetics Regulatory Sequences, Nucleic Acid - genetics Sequence Homology, Nucleic Acid Shine–Dalgarno sequence Transcription, Genetic - radiation effects untranslated region UTR
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Summary:	Most plastid promoters recognized by bacteria-like plastid RNA polymerase (PEP) are similar to E. coli σ70-type promoters comprising “–35” and “–10” elements. Among them, psbA promoter is unique in bearing additional elements between the conserved –35 and –10 elements. The psbA promoter activity is differentially maintained in the mature chloroplasts where the activity of most PEP promoters declines. Previously, we identified two types of PEP activities in wheat seedlings [Satoh et al. (1999) Plant J. 18: 407]; PEP present in the mature chloroplasts of the leaf tip (tip-type PEP) can initiate transcription from the –35-destructed psbA promoter, but the –35 element is essential for transcription by PEP present in immature chloroplasts of the leaf base (base-type PEP). To reveal which type of PEP functions in various types of plastids in tobacco, we analyzed the tobacco psbA promoter by means of a transplastomic approach. The promoter core context (–42 to +9) was sufficient for developmental regulation of the psbA promoter activity. The –35 promoter element was important for transcription initiation at the psbA promoter in all types of plastids, including chloroplasts in mature leaves, leucoplasts in roots, etioplasts in etiolated cotyledons. The conclusion is that the PEP bearing a promoter preference, similar to the wheat base-type PEP, functions dominantly in tobacco chloroplasts.
Bibliography:	2004001701 F30 local:pcg041 Received August 16, 2002; Accepted January 7, 2003 ark:/67375/HXZ-7CWJZSNX-R istex:537BE0B8726A21CB51DD38D16B9B3250519729A7 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0032-0781 1471-9053
DOI:	10.1093/pcp/pcg041