Effect of selected bioactive substances and nanoparticles on the immunoreactivity of edible packages containing chitosan, by the ELISA method

Effect of selected bioactive substances and nanoparticles on the immunoreactivity of edible packages containing chitosan, by the ELISA method

The aim of this study was evaluated the effect of selected bioactive substances and nanoparticles on the immunoreactivity of edible packages based on chitosan using the ELISA. The analysed protein was the tropomyosin. The results confirmed the presence of the tropomyosin (3.77 ± 0.79-5.75 ± 0.01 µg/...

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Bibliographic Details
Published in:Food and agricultural immunology Vol. 34; no. 1
Main Authors: Havlová, L., Pospiech, M., Javůrková, Z., Bartlová, M., Těšíková, K., Dordevic, D., Dordevic, S., Zemancová, J., Tremlová, B.
Format: Journal Article
Language:English
Published: Abingdon Taylor & Francis 31-12-2023
Taylor & Francis Ltd
Taylor & Francis Group
Subjects:
Biological activity
Chitosan
Edible packaging
ELISA
Enzyme-linked immunosorbent assay
grape extract
Grapes
Immunoreactivity
Nanoparticles
Packages
Pollen
Silver
Titanium dioxide
Tropomyosin
Zinc oxide
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Summary:The aim of this study was evaluated the effect of selected bioactive substances and nanoparticles on the immunoreactivity of edible packages based on chitosan using the ELISA. The analysed protein was the tropomyosin. The results confirmed the presence of the tropomyosin (3.77 ± 0.79-5.75 ± 0.01 µg/g) in control samples. This study demonstrated that the bioactive substances in the form of grape (0.61 ± 0.34-0.43 ± 0.16 µg/g), blueberry (0.58 ± 0.32-0.39 ± 0.27 µg/g), and parsley extracts (2.09 ± 1.28-0.79 ± 0.40 µg/g) reduces immunoreactivity (p < 0.05) of the tropomyosin. The elder pollen had no significant effect (p > 0.05) on immunoreactivity. ZnO and TiO 2 nanoparticles also demonstrated immunoreactivity reduction (p < 0.05). The exception was silver nanoparticles, where the immunoreactivity increased with increasing concentration of grape extract (1.29 ± 0.01-5.47 ± 0.25 µg/g). The results confirmed the inhibitory effect of bioactive substances on the immunoreactivity of the used ELISA and also showed the need to consider immunoreactive substances when interpreting ELISA results.
ISSN:0954-0105
1465-3443
DOI:10.1080/09540105.2023.2222933