Search Results - "Haugland, Rosaria P."

Quantitative Comparison of Long-wavelength Alexa Fluor Dyes to Cy Dyes: Fluorescence of the Dyes and Their Bioconjugates by Berlier, Judith E, Rothe, Anca, Buller, Gayle, Bradford, Jolene, Gray, Diane R, Filanoski, Brian J, Telford, William G, Yue, Stephen, Liu, Jixiang, Cheung, Ching-Ying, Chang, Wesley, Hirsch, James D, Beechem, Joseph M, Haugland, Rosaria P, Haugland, Richard P

“…Amine-reactive N-hydroxysuccinimidyl esters of Alexa Fluor fluorescent dyes with principal absorption maxima at about 555 nm, 633 nm, 647 nm, 660 nm, 680 nm,…”
Get full text

Easily reversible desthiobiotin binding to streptavidin, avidin, and other biotin-binding proteins: uses for protein labeling, detection, and isolation by Hirsch, James D, Eslamizar, Leila, Filanoski, Brian J, Malekzadeh, Nabi, Haugland, Rosaria P, Beechem, Joseph M, Haugland, Richard P

“…The high-affinity binding of biotin to avidin, streptavidin, and related proteins has been exploited for decades. However, a disadvantage of the…”
Get full text

Alexa Dyes, a Series of New Fluorescent Dyes that Yield Exceptionally Bright, Photostable Conjugates by Panchuk–Voloshina, Nataliya, Haugland, Rosaria P, Bishop–Stewart, Janell, Bhalgat, Mahesh K, Millard, Paul J, Mao, Fei, Leung, Wai-Yee, Haugland, Richard P

“…Alexa 350, Alexa 430, Alexa 488, Alexa 532, Alexa 546, Alexa 568, and Alexa 594 dyes are a new series of fluorescent dyes with emission/excitation spectra…”
Get full text

Conjugation of antibodies to biotin by Hirsch, James D, Haugland, Rosaria P

“…Antibodies can be conjugated to biotin by a number of chemical means. They can then be used in immunochemical procedures in conjunction with secondary reagents…”
Get more information

Texas Red-X and Rhodamine Red-X, New Derivatives of Sulforhodamine 101 and Lissamine Rhodamine B with Improved Labeling and Fluorescence Properties by Lefevre, Charles, Kang, Hee Chol, Haugland, Rosaria P, Malekzadeh, Nabi, Arttamangkul, Seksiri, Haugland, Richard P

“…Texas Red sulfonyl chloride (TR-SC) and Lissamine rhodamine B sulfonyl chloride (LRB-SC) are popular dyes often used to prepare red fluorescent conjugates that…”
Get full text

p75 and TrkA receptors are both required for uptake of NGF in adult sympathetic neurons: use of a novel fluorescent NGF conjugate by Gatzinsky, Kliment P, Haugland, Rosaria P, Thrasivoulou, Christopher, Orike, Nina, Budi-Santoso, Agus W, Cowen, Timothy

“…We have developed and tested the biological activity and specificity of a novel fluorescent dextran-Texas Red–nerve growth factor (DTR–NGF) conjugate. DTR–NGF…”
Get full text

Green- and red-fluorescent nanospheres for the detection of cell surface receptors by flow cytometry by Bhalgat, Mahesh K., Haugland, Rosaria P., Pollack, Jeffrey S., Swan, Sharon, Haugland, Richard P.

“…Fluorescent probes serve as sensitive tools for obtaining structural and functional information in cellular systems. In spite of the high sensitivity provided…”
Get full text

Phallotoxin and actin binding assay by fluorescence enhancement by Huang, Z J, Haugland, R P, You, W M

“…The fluorescence of five fluorophores conjugated to phallotoxins was found to be specifically enhanced upon binding to F-actin in a polymerizing buffer…”
Get more information

Vitronectin Interaction with Glycosaminoglycans by François, Patrice P., Preissner, Klaus T., Herrmann, Mathias, Haugland, Rosaria P., Vaudaux, P., Lew, Daniel P., Krause, Karl-Heinz

“…Vitronectin (VN) is a high affinity heparin-binding protein. The physiological role of this binding has hitherto received little attention, and its molecular…”
Get full text

7-Amino-4-methyl-6-sulfocoumarin-3-acetic acid: a novel blue fluorescent dye for protein labeling by Leung, Wai-Yee, Trobridge, Patty A., Haugland, Rosaria P., Haugland, Richard P., Mao, Fei

“…7-Amino-4-methyl-6-sulfocoumarin-3-acetic acid (AMCA-S, also called Alexa ™ 350) 2 was synthesized as a new water-soluble blue fluorescent dye for protein…”
Get full text

Coupling of antibodies with biotin by Haugland, Rosaria P, You, Wendy W

“…The avidin-biotin bond is the strongest known biological interaction between a ligand and a protein (Kd = 1.3 x 10-15 M at pH 5.0) (1). The affinity is so high…”
Get more information

Preparation of avidin conjugates by Haugland, Rosaria P, Bhalgat, Mahesh K

“…The high-affinity avidin-biotin system has found applications in different fields of biotechnology, including immunoassays, histochemistry, affinity…”
Get more information

Quenched BODIPY Dye-Labeled Casein Substrates for the Assay of Protease Activity by Direct Fluorescence Measurement by Jones, Laurie J., Upson, Rosalyn H., Haugland, Rosaria P., Panchuk-Voloshina, Nataliya, Zhou, Mingjie, Haugland, Richard P.

“…We have prepared casein conjugates of two BODIPY dyes for use as fluorogenic protease substrates in homogeneous assays. Both conjugates are labeled to such an…”
Get full text

3-(4-Carboxybenzoyl)quinoline-2-carboxaldehyde, a Reagent with Broad Dynamic Range for the Assay of Proteins and Lipoproteins in Solution by You, Wendy W., Haugland, Rosaria P., Ryan, Diane K., Haugland, Richard P.

“…This paper describes the application of 3-(4-carboxybenzoyl)quinoline-2-carboxaldehyde (CBQCA), a sensitive fluorogenic reagent for detection of amines, to the…”
Get full text

A Spectrophotometric Method to Measure Enzymatic Activity in Reactions That Generate Inorganic Pyrophosphate by Upson, Rosalyn H., Haugland, Rosaria P., Malekzadeh, Mohammad N., Haugland, Richard P.

“…This paper describes a spectrophotometric assay that can measure the inorganic pyrophosphate produced from various enzymatic reactions. This is a coupled assay…”
Get full text

Vitronectin interaction with glycosaminoglycans. Kinetics, structural determinants, and role in binding to endothelial cells by François, P P, Preissner, K T, Herrmann, M, Haugland, R P, Vaudaux, P, Lew, D P, Krause, K H

“…Vitronectin (VN) is a high affinity heparin-binding protein. The physiological role of this binding has hitherto received little attention, and its molecular…”
Get full text

Fluorescent rhodol derivatives: versatile, photostable labels and tracers by Whitaker, J E, Haugland, R P, Ryan, D, Hewitt, P C, Prendergast, F G

“…A series of chemically reactive, fluorescent rhodol derivatives was prepared and evaluated. Reactive functional groups included activated esters, amines,…”
Get more information

Green/red dual fluorescence detection of total protein and alkaline phosphate-conjugated probes on blotting membranes by Top, Karen Pretty On, Hatleberg, Gayle, Berggren, Kiera N., Ryan, Diane, Kemper, Courtenay, Haugland, Rosaria P., Patton, Wayne F.

“…A two‐color fluorescence detection method is described based upon covalently coupling the succinimidyl ester of BODIPY FL‐X to proteins immobilized on…”
Get full text

5-(Pentafluorobenzoylamino)fluorescein: A selective substrate for the determination of glutathione concentration and glutathione S-transferase activity by Arttamangkul, S, Bhalgat, M K, Haugland, R P, Diwu, Z, Liu, J, Klaubert, D H

“…5-(Pentafluorobenzoylamino)fluorescein (PFB-F), a new thiol-reactive molecule was synthesized to improve the detection limits and specificity of the assays for…”
Get full text

Antibody conjugates for cell biology by Haugland, R P

“…Antibody conjugates are extremely useful reagents for probing many biologically and chemically important molecules in vitro or in vivo. This unit presents some…”
Get more information