Transplantation of R-GSIK scaffold with mesenchymal stem cells improves neuroinflammation in a traumatic brain injury model

Transplantation of R-GSIK scaffold with mesenchymal stem cells improves neuroinflammation in a traumatic brain injury model

Neural tissue engineering has been introduced as a novel therapeutic strategy for traumatic brain injury (TBI). Transplantation of mesenchymal stem cells (MSCs) has been demonstrated to improve functional outcome of brain injury, and RADA4GGSIKVAV (R-GSIK), a self-assembling nano-peptide scaffold, h...

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Bibliographic Details
Published in:Cell and tissue research Vol. 382; no. 3; pp. 575 - 583
Main Authors: Sahab Negah, Sajad, Shirzad, Mohammad Moein, Biglari, Ghazale, Naseri, Farzin, Hosseini Ravandi, Hassan, Hassani Dooghabadi, Ali, Gorji, Ali
Format: Journal Article
Language:English
Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-12-2020
Springer
Springer Nature B.V
Subjects:
Analysis
Animals
Astrocytes
Biomedical and Life Sciences
Biomedicine
Brain
Brain Injuries, Traumatic - pathology
Brain Injuries, Traumatic - therapy
Cell Differentiation - physiology
Disease Models, Animal
Ethylenediaminetetraacetic acid
Human Genetics
Immunomodulation
Inflammation
Injuries
Interleukin 6
Male
Mesenchymal Stem Cell Transplantation - methods
Mesenchymal stem cells
Mesenchymal Stem Cells - cytology
Microglial cells
Molecular Medicine
Peptides
Peptides - administration & dosage
Proteomics
Rats
Rats, Wistar
Regular
Regular Article
Stem cell transplantation
Stem cells
Tissue engineering
Tissue Scaffolds - standards
TLR4 protein
Toll-like receptors
Transplantation
Traumatic brain injury
Tumor necrosis factor
Neuroinflammation
Head injury
Nano-scaffold
Stem cells
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Summary:Neural tissue engineering has been introduced as a novel therapeutic strategy for traumatic brain injury (TBI). Transplantation of mesenchymal stem cells (MSCs) has been demonstrated to improve functional outcome of brain injury, and RADA4GGSIKVAV (R-GSIK), a self-assembling nano-peptide scaffold, has been suggested to promote the behavior of stem cells. This study was designed to determine the ability of the R-GSIK scaffold in supporting the effects of MSCs on motor function activity and inflammatory responses in an experimental TBI model. A significant recovery of motor function was observed in rats that received MSCs+R-GSIK compared with the control groups. Further analysis showed a reduction in the number of reactive astrocytes and microglial cells in the MSCs and MSCs+R-GSIK groups compared with the control groups. Furthermore, western blot analysis indicated a significant reduction in pro-inflammatory cytokines, such as TLR4, TNF, and IL6, in the MSCs and MSCs+R-GSIK groups compared with the TBI, vehicle, and R-GSIK groups. Overall, this study strengthens the idea that the co-transplantation of MSCs with R-GSIK can increase functional outcomes by preparing a beneficial environment. This improvement may be explained by the immunomodulatory effects of MSCs and the self-assembling nano-scaffold peptide.
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ISSN:0302-766X
1432-0878
1432-0878
DOI:10.1007/s00441-020-03247-0