Search Results - "Haselberg, Rob"

High-resolution glycoform profiling of intact therapeutic proteins by hydrophilic interaction chromatography-mass spectrometry by Domínguez-Vega, Elena, Tengattini, Sara, Peintner, Claudia, van Angeren, Jordy, Temporini, Caterina, Haselberg, Rob, Massolini, Gabriella, Somsen, Govert W.

“…Glycosylation is considered a critical quality attribute of therapeutic proteins. Protein heterogeneity introduced by glycosylation includes differences in the…”
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CE-MS for the analysis of intact proteins 2010-2012 by Haselberg, Rob, de Jong, Gerhardus J., Somsen, Govert W.

“…Since its introduction in 1987, CE‐MS has become an increasingly important technique for the analysis of biomolecules. Since our previous update on CE‐MS…”
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Studying protein structure and function by native separation–mass spectrometry by van Schaick, Guusje, Haselberg, Rob, Somsen, Govert W., Wuhrer, Manfred, Domínguez-Vega, Elena

“…Alterations in protein structure may have profound effects on biological function. Analytical techniques that permit characterization of proteins while…”
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Microfluidic ion stripper for removal of trifluoroacetic acid from mobile phases used in HILIC-MS of intact proteins by Wouters, Sam, Eeltink, Sebastiaan, Haselberg, Rob, Somsen, Govert W., Gargano, Andrea F. G.

“…Trifluoroacetic acid (TFA) is commonly used as mobile phase additive to improve retention and peak shape characteristics in hydrophilic interaction liquid…”
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Mass spectrometry for glycosylation analysis of biopharmaceuticals by Dotz, Viktoria, Haselberg, Rob, Shubhakar, Archana, Kozak, Radoslaw P., Falck, David, Rombouts, Yoann, Reusch, Dietmar, Somsen, Govert W., Fernandes, Daryl L., Wuhrer, Manfred

“…•Biopharmaceuticals are very promising for the treatment of multiple diseases.•Therapeutic proteins often bear sugar chains that can influence their…”
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Capillary electrophoresis–mass spectrometry for the analysis of intact proteins by Haselberg, Rob, de Jong, Gerhardus J., Somsen, Govert W.

“…Developments in the fields of protein chemistry, proteomics and biotechnology have increased the demand for suitable analytical techniques for the analysis of…”
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Performance of a sheathless porous tip sprayer for capillary electrophoresis–electrospray ionization-mass spectrometry of intact proteins by Haselberg, Rob, Ratnayake, Chitra K., de Jong, Gerhardus J., Somsen, Govert W.

“…The performance of a prototype porous tip sprayer for sheathless capillary electrophoresis–mass spectrometry (CE–MS) of intact proteins was studied…”
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Limited Lactosylation of Beta-Lactoglobulin from Cow’s Milk Exerts Strong Influence on Antigenicity and Degranulation of Mast Cells by Bosman, Gerlof P, Oliveira, Sergio, Simons, Peter J, Sastre Torano, Javier, Somsen, Govert W, Knippels, Leon M. J, Haselberg, Rob, Pieters, Roland J, Garssen, Johan, Knipping, Karen

“…Background: beta-lactoglobulin (BLG) is one of the major cow’s milk proteins and the most abundant allergen in whey. Heating is a common technologic treatment…”
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A Novel Platinum(II)-Based Bifunctional ADC Linker Benchmarked Using 89Zr-Desferal and Auristatin F-Conjugated Trastuzumab by Sijbrandi, Niels J, Merkul, Eugen, Muns, Joey A, Waalboer, Dennis C J, Adamzek, Kevin, Bolijn, Marije, Montserrat, Veronica, Somsen, Govert W, Haselberg, Rob, Steverink, Paul J G M, Houthoff, Hendrik-Jan, van Dongen, Guus A M S

“…Greater control is desirable in the stochastic conjugation technology used to synthesize antibody-drug conjugates (ADC). We have shown recently that a…”
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Developments in Interfacing Designs for CE–MS: Towards Enabling Tools for Proteomics and Metabolomics by Lindenburg, Petrus W., Haselberg, Rob, Rozing, Gerard, Ramautar, Rawi

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Binding Properties 2022 by Haselberg, Rob, Stein, Matthias

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Capillary Electrophoresis: Trends and Recent Advances by Voeten, Robert L. C, Ventouri, Iro K, Haselberg, Rob, Somsen, Govert W

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Hydrophilic interaction chromatography – mass spectrometry for metabolomics and proteomics: state-of-the-art and current trends by Kohler, Isabelle, Verhoeven, Michel, Haselberg, Rob, Gargano, Andrea F.G.

“…[Display omitted] Among all the –omics approaches, proteomics and metabolomics have received increased attention over the last decade. Both approaches have…”
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Low-Flow Sheathless Capillary Electrophoresis–Mass Spectrometry for Sensitive Glycoform Profiling of Intact Pharmaceutical Proteins by Haselberg, Rob, de Jong, Gerhardus J, Somsen, Govert W

“…Capillary electrophoresis coupled to time-of-flight mass spectrometry (CE–TOF-MS) via a porous tip sheathless electrospray ionization (ESI) interface was…”
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Developments in Interfacing Designs for CE–MS: Towards Enabling Tools for Proteomics and Metabolomics by Lindenburg, Petrus W., Haselberg, Rob, Rozing, Gerard, Ramautar, Rawi

“…Capillary electrophoresis–mass spectrometry (CE–MS) can be considered a useful analytical technique for the analysis of charged compounds in the fields of…”
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Analysis of microperoxidases using liquid chromatography, post-column substrate conversion and fluorescence detection by Haselberg, Rob, Hempen, Christel, van Leeuwen, Suze M., Vogel, Martin, Karst, Uwe

“…A liquid chromatographic method with on-line activity determination for microperoxidases has been developed. After enzymatic digestion of a cytochrome,…”
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Affinity profiling of monoclonal antibody and antibody-drug-conjugate preparations by coupled liquid chromatography-surface plasmon resonance biosensing by Lakayan, Dina, Haselberg, Rob, Gahoual, Rabah, Somsen, Govert W., Kool, Jeroen

“…Monoclonal antibodies (mAbs) and antibody-drug conjugates (ADCs) are highly potent biopharmaceuticals designed for targeted cancer therapies. mAbs and ADCs can…”
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Online multimethod platform for comprehensive characterization of monoclonal antibodies in cell culture fluid from a single sample injection - Intact protein workflow by Sadighi, Raya, de Kleijne, Vera, Wouters, Sam, Lubbers, Karin, Somsen, Govert W, Gargano, Andrea F G, Haselberg, Rob

“…Therapeutic monoclonal antibodies (mAbs) comprise a large structural variability with respect to charge, size and post-translational modifications. These…”
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Characterizing Non-covalent Protein Complexes Using Asymmetrical Flow Field-Flow Fractionation On-Line Coupled to Native Mass Spectrometry by Ventouri, Iro Konstantina, Chang, Wayne, Meier, Florian, Drexel, Roland, Somsen, Govert W., Schoenmakers, Peter J., de Spiegeleer, Bart, Haselberg, Rob, Astefanei, Alina

“…We report an online analytical platform based on the coupling of asymmetrical flow field-flow fractionation (AF4) and native mass spectrometry (nMS) in…”
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Heterogeneity assessment of antibody-derived therapeutics at the intact and middle-up level by low-flow sheathless capillary electrophoresis-mass spectrometry by Haselberg, Rob, De Vijlder, Thomas, Heukers, Raimond, Smit, Martine J., Romijn, Edwin P., Somsen, Govert W., Domínguez-Vega, Elena

“…Antibody-based pharmaceuticals often encompass a complex structural heterogeneity requiring enhanced analytical methods for reliable characterization of…”
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