Search Results - "Hartman, F C"

Structure of yeast triosephosphate isomerase at 1.9-Å resolution by LOLIS, E, ALBER, T, DAVENPORT, R. C, ROSE, D, HARTMAN, F. C, PETSKO, G. A

“…The structure of yeast triosephosphate isomerase (TIM) has been solved at 3.0-A resolution and refined at 1.9-A resolution to an R factor of 21.0%. The final…”
Get full text

Characterization of the regulatory thioredoxin site of phosphoribulokinase by Porter, M A, Stringer, C D, Hartman, F C

“…Phosphoribulokinase is light-regulated via thioredoxin by reversible oxidation/reduction of sulfhydryl/disulfide groups. To identify the cysteinyl residues…”
Get full text

Restoration of activity to catalytically deficient mutants of ribulosebisphosphate carboxylase/oxygenase by aminoethylation by Smith, H B, Hartman, F C

“…Substitutions for active-site lysyl residues at positions 166 and 329 in ribulosebisphosphate carboxylase/oxygenase from Rhodospirillum rubrum have been shown…”
Get full text

Enhanced CO2/O2 specificity of a site-directed mutant of ribulose-bisphosphate carboxylase/oxygenase by HARPEL, M. R, HARTMAN, F. C

“…The CO2/O2 specificity factor of ribulose-bisphosphate carboxylase/oxygenase partially determines the efficiency of photosynthetic carbon assimilation…”
Get full text

Beta-elimination of phosphate from reaction intermediates by site-directed mutants of ribulose-bisphosphate carboxylase/oxygenase by LARIMER, F. W, HARPEL, M. R, HARTMAN, F. C

“…Five residues (Thr-53, Asn-54, Gly-370, Gly-393, and Gly-394) of Rhodospirillum rubrum ribulose-bisphosphate carboxylase/oxygenase are positioned to serve as…”
Get full text

Evidence supporting lysine 166 of Rhodospirillum rubrum ribulosebisphosphate carboxylase as the essential base which initiates catalysis by Lorimer, G H, Hartman, F C

“…The epsilon-amino group of Lys-166 of Rhodospirillum rubrum ribulosebisphosphate carboxylase/oxygenase was postulated as the essential base which initiates…”
Get full text

Examination of the function of active site lysine 329 of ribulose-bisphosphate carboxylase/oxygenase as revealed by the proton exchange reaction by Hartman, F C, Lee, E H

“…Diverse approaches that include site-directed mutagenesis have indicated a catalytic role of Lys-329 of ribulosebisphosphate carboxylase/oxygenase from…”
Get full text

Roles of cysteinyl residues of phosphoribulokinase as examined by site-directed mutagenesis by MILANEZ, S, MURAL, R. J, HARTMAN, F. C

“…The Calvin Cycle enzyme phosphoribulokinase is activated in higher plants by the reversible reduction of a disulfide bond, which is located at the active site…”
Get full text

A role for the ε-amino group of lysine-334 of ribulose-1,5-bisphosphate carboxylase in the addition of carbon dioxide to the 2,3-enediol(ate) of ribulose 1,5-bisphosphate by LORIMER, G. H, YUH-RU CHEN, HARTMAN, F. C

“…Earlier structural and functional studies of ribulose-1,5-bisphosphate carboxylase/oxygenase imply that K334 facilitates the addition of gaseous substrate to…”
Get full text

Functional analysis of the putative catalytic bases His-321 and Ser-368 of Rhodospirillum rubrum ribulose bisphosphate carboxylase/oxygenase by site-directed mutagenesis by HARPEL, M. R, LARIMER, F. W, HARTMAN, F. C

“…Numerous candidates have been suggested according to chemical and structural criteria for the active site base of ribulose bisphosphate carboxylase/oxygenase…”
Get full text

Multiple catalytic roles of His 287 of Rhodospirillum rubrum ribulose 1,5‐bisphosphate carboxylase/oxygenase by Harpel, Mark R., Larimer, Frank W., Hartman, Fred C.

“…Active‐site His 287 of Rhodospirillum rubrum ribulose 1,5‐bisphosphate (RuBP) carboxylase/oxygenase interacts with the C3‐hydroxyl of bound substrate or…”
Get full text

Direct identification of the primary nucleophile of thioredoxin f by Brandes, H.K., Larimer, F.W., Geck, M.K., Stringer, C.D., Schürmann, P., Hartman, F.C.

“…Thioredoxin, by virtue of the proximal active-site sulfhydryls (Trp-Cys-Gly-Pro-Cys), catalyzes thiol-disulfide exchange with specific target enzymes…”
Get full text

D-Ribulose-5-Phosphate 3-Epimerase: Cloning and Heterologous Expression of the Spinach Gene, and Purification and Characterization of the Recombinant Enzyme by Yuh-Ru Chen, Hartman, Fred C., Tse-Yuan S. Lu, Larimer, Frank W.

“…We have achieved, to our knowledge, the first high-level heterologous expression of the gene encoding D-ribulose-5-phosphate 3-epimerase from any source,…”
Get full text

Intersubunit location of the active site of ribulose-bisphosphate carboxylase/oxygenase as determined by in vivo hybridization of site-directed mutants by Larimer, F W, Lee, E H, Mural, R J, Soper, T S, Hartman, F C

“…Ribulose bisphosphate carboxylase/oxygenase from Rhodospirillum rubrum is a homodimer of 50.5-kDa subunits with two substrate binding sites per molecule of…”
Get full text

Function of Lys-166 of Rhodospirillum rubrum ribulosebisphosphate carboxylase/oxygenase as examined by site-directed mutagenesis by Hartman, F.C., Soper, T.S., Niyogi, S.K., Mural, R.J., Foote, R.S., Mitra, S., Lee, E.H., Machanoff, R., Larimer, F.W.

“…Affinity labeling and comparative sequence analyses have placed Lys-166 of ribulosebisphosphate carboxylase/oxygenase from Rhodospirillum rubrum at the active…”
Get full text

Perturbation of reaction-intermediate partitioning by a site-directed mutant of ribulose-bisphosphate carboxylase/oxygenase by Lee, E H, Harpel, M R, Chen, Y R, Hartman, F C

“…To explore the roles of active-site Glu48 of ribulose-bisphosphate carboxylase/oxygenase from Rhodospirillum rubrum, the E48Q mutant has been characterized…”
Get full text

Catalytic nonessentiality of an active-site cysteinyl residue of phosphoribulokinase by Porter, M A, Hartman, F C

“…The activity of the Calvin cycle enzyme phosphoribulokinase is coupled to photosynthetic electron transport by reversible oxidation/reduction mediated by…”
Get full text

Nonessentiality of histidine 291 of Rhodospirillum rubrum ribulose-bisphosphate carboxylase/oxygenase as determined by site-directed mutagenesis by Niyogi, S K, Foote, R S, Mural, R J, Larimer, F W, Mitra, S, Soper, T S, Machanoff, R, Hartman, F C

“…Chemical modification of spinach ribulosebisphosphate carboxylase/oxygenase by diethyl pyrocarbonate led to the conclusion that His-298 is an essential…”
Get full text

Examination of the intersubunit interaction between glutamate-48 and lysine-168 of ribulose-bisphosphate carboxylase/oxygenase by site-directed mutagenesis by MURAL, R. J, SOPER, T. S, LARIMER, F. W, HARTMAN, F. C

“…The active site of ribulose-bisphosphate carboxylase/oxygenase is constituted from domains of adjacent subunits and includes an intersubunit electrostatic…”
Get full text

Anion-exchange analysis of ribulose-bisphosphate carboxylase/oxygenase reactions: CO2/O2 specificity determination and identification of side products by Harpel, M R, Lee, E H, Hartman, F C

“…An improved anion-exchange chromatographic method for determining the carboxylation/oxygenation specificity (tau) of ribulose 1,5-bisphosphate…”
Get more information