Search Results - "Hartley, Brian S"
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Glucose isomerase: insights into protein engineering for increased thermostability
Published in Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular Enzymology (29-12-2000)“…Thermostable glucose isomerases are desirable for production of 55% fructose syrups at >90°C. Current commercial enzymes operate only at 60°C to produce 45%…”
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Book Review Journal Article -
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Active site titration and aminoacyl adenylate binding stoichiometry of aminoacyl-tRNA synthetases
Published in Biochemistry (Easton) (14-01-1975)“…A simple, rapid, and economical procedure is described for the determination of the number of catalytically competent active sites on aminoacyl-tRNA…”
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Saccharomyces cerevisiae cells secreting an Aspergillus niger beta-galactosidase grow on whey permeate
Published in Bio/technology (New York, N.Y. 1983) (01-01-1992)“…We describe the construction of a lactose-utilizing Saccharomyces cerevisiae that expresses the cDNA for a secreted, thermostable beta-galactosidase (lacA)…”
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The amino acid sequence of ribitol dehydrogenase-F, a mutant enzyme with improved xylitol dehydrogenase activity
Published in Journal of Protein Chemistry (01-05-1999)“…A mutant ribitol dehydrogenase (RDH-F) was purified from Klebsiella aerogenes strain F which evolved from the wild-type strain A under selective pressure to…”
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Gene duplication in experimental enzyme evolution
Published in Nature (London) (20-09-1974)Get full text
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Extraction and rapid inactivation of proteins from Saccharomyces cerevisiae by trichloroacetic acid precipitation
Published in Yeast (Chichester, England) (01-01-1989)“…Methods currently used for the extraction of proteins from yeast involve relatively long time periods between sampling cells from a culture and analysis of…”
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A Two-Dimensional System for the Separation of Ammo-Acids and Peptides on Paper
Published in Nature (London) (12-12-1959)Get full text
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A comparison of wild-type and mutant ribitol dehydrogenases from Klebsiella aerogenes
Published in Biochemical journal (01-11-1974)“…A ribitol dehydrogenase (ribitol-NAD(+) oxidoreductase, EC. 1.1.1.56) having increased specificity and catalytic efficiency toward xylitol was isolated from…”
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Fermentation of lactose by yeast cells secreting recombinant fungal lactase
Published in Applied and Environmental Microbiology (01-12-1993)“…Strains of Saccharomyces cerevisiae transformed with a yeast multicopy expression vector carrying the cDNA for Aspergillus niger secretory beta-galactosidase…”
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Ribitol dehydrogenase from Klebsiella aerogenes. Purification and subunit structure
Published in Biochemical journal (01-09-1974)“…Ribitol dehydrogenase has been purified to homogeneity from several strains of Klebsiella aerogenes. One strain yields 3-6g of pure enzyme from 1kg of cells…”
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The Amino Acid Sequence of the Tyrosy1‐tRNA Synthetase from Bacillus stearothermophilus
Published in European journal of biochemistry (01-05-1983)“…The primary structure of the tyrosy1‐RNA synthetase(Tyr Ts) of Bacillus stearothermophilus has been dedced from the nucleotide sequence of the cloned gene and…”
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Linked scans of peptides and protein digests: amino acid sequence determination of components of complex mixtures
Published in Rapid communications in mass spectrometry (01-04-1989)“…Two-sector linked-scan analysis of an unpurified proteolytic digest of a pyruvate decarboxylase enzyme (60,000 Da) has allowed the discovery and assignment of…”
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Transcription of the Bacillus subtilis Bacteriophage o3T in vitro
Published in Journal of general virology (01-09-1985)“…Centre for Biotechnology, Imperial College, London SW7 2AZ and 1 Microbial Technology Laboratory, PHLS Centre for Applied Microbiology and Research, Porton…”
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A Host-Vector System for an Arthrobacter Species
Published in Journal of general microbiology (01-04-1988)“…Centre for Biotechnology, Imperial College of Science and Technology, London SW7 2AZ, UK ABSTRACT SUMMARY: An efficient host-vector system has been developed…”
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A Switch from Translational Control to Transcriptional Control of Protein Synthesis in Mid‐exponential Growth Phase of Bacterial Cultures
Published in European journal of biochemistry (01-09-1980)“…1. We present evidence suggesting a sudden switch from translational control to transcriptional control of protein synthesis in mid‐exponential growth of…”
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Identification, cloning and characterisation of a new gene required for full pyruvate decarboxylase activity in Saccharomyces cerevisiae
Published in Current genetics (01-03-1989)“…Biochemical evidence that pyruvate decarboxylase in S. cerevisiae might be constituted from two independently encoded subunits led us to question genetic…”
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