Search Results - "Harris, Nicola J."

Cell-free expression tools to study co-translational folding of alpha helical membrane transporters by Harris, Nicola J., Pellowe, Grant A., Booth, Paula J.

“…Most helical membrane proteins fold co-translationally during unidirectional polypeptide elongation by the ribosome. Studies thus far, however, have largely…”
Get full text

Hydrogen-deuterium exchange mass spectrometry captures distinct dynamics upon substrate and inhibitor binding to a transporter by Jia, Ruyu, Martens, Chloe, Shekhar, Mrinal, Pant, Shashank, Pellowe, Grant A., Lau, Andy M., Findlay, Heather E., Harris, Nicola J., Tajkhorshid, Emad, Booth, Paula J., Politis, Argyris

“…Proton-coupled transporters use transmembrane proton gradients to power active transport of nutrients inside the cell. High-resolution structures often fail to…”
Get full text

In vitro synthesis of a Major Facilitator Transporter for specific active transport across Droplet Interface Bilayers by Findlay, Heather E., Harris, Nicola J., Booth, Paula J.

“…Nature encapsulates reactions within membrane-bound compartments, affording sequential and spatial control over biochemical reactions. Droplet Interface…”
Get full text

Structure formation during translocon-unassisted co-translational membrane protein folding by Harris, Nicola J., Reading, Eamonn, Ataka, Kenichi, Grzegorzewski, Lucjan, Charalambous, Kalypso, Liu, Xia, Schlesinger, Ramona, Heberle, Joachim, Booth, Paula J.

“…Correctly folded membrane proteins underlie a plethora of cellular processes, but little is known about how they fold. Knowledge of folding mechanisms centres…”
Get full text

Methods to study folding of alpha-helical membrane proteins in lipids by Harris, Nicola J, Pellowe, Grant A, Blackholly, Laura R, Gulaidi-Breen, Samuel, Findlay, Heather E, Booth, Paula J

“…How alpha-helical membrane proteins fold correctly in the highly hydrophobic membrane interior is not well understood. Their folding is known to be highly…”
Get full text

Cell-Free Expression to Probe Co-Translational Insertion of an Alpha Helical Membrane Protein by Blackholly, Laura R, Harris, Nicola J, Findlay, Heather E, Booth, Paula J

“…The majority of alpha helical membrane proteins fold co-translationally during their synthesis on the ribosome. In contrast, most mechanistic folding studies…”
Get full text

Cell-Free Synthesis Strategies to Probe Co-translational Folding of Proteins Within Lipid Membranes by Harris, Nicola J, Reading, Eamonn, Booth, Paula J

“…In order to comprehend the molecular basis of transmembrane protein biogenesis, methods are required that are capable of investigating the co-translational…”
Get more information

Co-Translational Protein Folding in Lipid Membranes by Harris, Nicola J., Booth, Paula J.

Get full text

How lipids affect the energetics of co-translational alpha helical membrane protein folding by Brady, Ryan, Harris, Nicola J, Pellowe, Grant A, Gulaidi Breen, Samuel, Booth, Paula J

“…Membrane proteins need to fold with precision in order to function correctly, with misfolding potentially leading to disease. The proteins reside within a…”
Get more information

Folding and stability of membrane transport proteins in vitro by Harris, Nicola J., Booth, Paula J.

“…Transmembrane transporters are responsible for maintaining a correct internal cellular environment. The inherent flexibility of transporters together with…”
Get full text

Integrating Membrane Transporter Proteins into Droplet Interface Bilayers by Findlay, Heather E, Harris, Nicola J, Booth, Paula J

“…Droplet interface bilayers (DIBs) are an emerging tool within synthetic biology that aims to recreate biological processes in artificial cells. A critical…”
Get more information

Amyloid-like Fibrils from an α‑Helical Transmembrane Protein by Stroobants, Karen, Kumita, Janet R, Harris, Nicola J, Chirgadze, Dimitri Y, Dobson, Christopher M, Booth, Paula J, Vendruscolo, Michele

“…The propensity to misfold and self-assemble into stable aggregates is increasingly being recognized as a common feature of protein molecules. Our understanding…”
Get full text

Relative Domain Folding and Stability of a Membrane Transport Protein by Harris, Nicola J., Findlay, Heather E., Simms, John, Liu, Xia, Booth, Paula J.

“…There is a limited understanding of the folding of multidomain membrane proteins. Lactose permease (LacY) of Escherichia coli is an archetypal member of the…”
Get full text

Waste biomass from hypersaline potash mining byproducts: Detection and visualization of Cu(II) and Cr(VI) on Croceicoccus sp. FTI14 biosorbent by Harris, Nicola J., Dynes, James J., McBeth, Joyce M., Patel, Manvendra, Chang, Wonjae

“…Microorganisms in hypersaline potash mining byproducts and their potential environmental applications have not been extensively reported. This study reports…”
Get full text

Comparative stability of Major Facilitator Superfamily transport proteins by Harris, Nicola J., Findlay, Heather E., Sanders, Michael R., Kedzierski, Mateusz, dos Santos, Ália, Booth, Paula J.

“…Membrane transporters are a vital class of proteins for which there is little available structural and thermodynamic information. The Major Facilitator…”
Get full text

Investigating the Insertion and Folding of Membrane Transporters into Lipid Bilayers using a Cell Free Expression System by Harris, Nicola J., Booth, Paula J.

Get full text

Investigations into the Reversible Folding of the Two Domain Helical Transporter Lactose Permease by Harris, Nicola J., Booth, Paula J.

Get full text