Effectiveness of egg yolk immunoglobulin against the intracellular salmonid pathogen Piscirickettsia salmonis

Aims To produce and characterize egg yolk immunoglobulin (IgY) against the fish intracellular pathogen Piscirickettsia salmonis as well as to evaluate the antibacterial activity of IgY in vitro and the availability in the serum of fish immunized orally. Methods and Results Specific IgY was produced...

Full description

Saved in:

Bibliographic Details
Published in:	Journal of applied microbiology Vol. 119; no. 2; pp. 365 - 376
Main Authors:	Oliver, C., Valenzuela, K., Silva, H., Haro, R.E., Cortés, M., Sandoval, R., Pontigo, J.P., Álvarez, C., Figueroa, J.E., Avendaño‐Herrera, R., Troncoso, J.M., Yáñez, A.J.
Format:	Journal Article
Language:	English
Published:	England Oxford University Press 01-08-2015
Subjects:	Animals Anti-Bacterial Agents - isolation & purification Anti-Bacterial Agents - pharmacology chicken egg yolk Chickens - immunology Egg Yolk - chemistry Electrophoresis, Polyacrylamide Gel Fish Diseases - drug therapy Fish Diseases - immunology Fish Diseases - microbiology growth inhibition IgY immunoglobulin Immunoglobulins Immunoglobulins - isolation & purification Immunoglobulins - pharmacology Microbiology Microorganisms Pathogens Piscirickettsia - drug effects Piscirickettsia - growth & development Piscirickettsia salmonis Piscirickettsiaceae Infections - drug therapy Piscirickettsiaceae Infections - immunology Piscirickettsiaceae Infections - microbiology Piscirickettsiaceae Infections - veterinary piscirickettsiosis Salmo salar Salmo salar - microbiology Salmon Piscirickettsia salmonis IgY growth inhibition immunoglobulin chicken egg yolk piscirickettsiosis
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Aims To produce and characterize egg yolk immunoglobulin (IgY) against the fish intracellular pathogen Piscirickettsia salmonis as well as to evaluate the antibacterial activity of IgY in vitro and the availability in the serum of fish immunized orally. Methods and Results Specific IgY was produced by immunizing hens with P. salmonis proteins. The IgY was obtained from egg yolks using the ammonium sulphate precipitation method and it was characterized by SDS‐PAGE, Western‐blot and ELISA, demonstrating that anti‐P. salmonis IgY strongly reacted specifically against P. salmonis proteins. In an in vitro neutralization assay, IgY inhibited the growth of P. salmonis in liquid medium at concentrations ranging from 128 to 256 μg ml−1 in a dose‐dependent manner. Interestingly, IgY against P. salmonis also generates a strong protective effect on the infection of P. salmonis in salmon head kidney‐1 cells. In addition, the bacteriostatic function of IgY appears to result possibly from agglutination by the interaction of IgY with surface components of the pathogen. Finally, to confirm this IgY as an alternative for salmonid treatment, Atlantic salmon (Salmo salar) specimens were orally inoculated with IgY. The analysis of the sera demonstrates that IgY was effectively transported by fish intestine and that this immunoglobulins maintains its properties and recognizes several proteins of P. salmonis up to 12 h after inoculation of IgY against P. salmonis. Conclusions Specific IgY effectively inhibited the growth of P. salmonis and this immunoglobulin can be released in the Atlantic salmon sera when administered orally to fish. Significance and Impact of the Study We propose that this specific IgY against this fastidious micro‐organism could be a useful strategy for the treatment of piscirickettsiosis.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1364-5072 1365-2672
DOI:	10.1111/jam.12857