Search Results - "Harmsen, M.M."

Stabilizing effects of excipients on dissociation of intact (146S) foot-and-mouth disease virions into 12S particles during storage as oil-emulsion vaccine by Harmsen, M.M, Fijten, H.P.D, Westra, D.F, Dekker, A

“…Highlights • We developed methods for measuring FMDV virion stability in vaccines by ELISA. • We followed FMDV virion stability in oil-adjuvanted vaccines…”
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Effect of thiomersal on dissociation of intact (146S) foot-and-mouth disease virions into 12S particles as assessed by novel ELISAs specific for either 146S or 12S particles by Harmsen, M.M, Fijten, H.P.D, Westra, D.F, Coco-Martin, J.M

“…Abstract Intact (146S) foot-and-mouth disease virions (FMDVs) can dissociate into specific (12S) viral capsid degradation products. Using two single-domain…”
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Adaptation of a Madin–Darby canine kidney cell line to suspension growth in serum-free media and comparison of its ability to produce avian influenza virus to Vero and BHK21 cell lines by van Wielink, R., Kant-Eenbergen, H.C.M., Harmsen, M.M., Martens, D.E., Wijffels, R.H., Coco-Martin, J.M.

“…Madin–Darby canine kidney (MDCK) cells are currently considered for influenza vaccine manufacturing. A drawback of these cells is their anchorage dependent…”
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MDCK cell line with inducible allele B NS1 expression propagates delNS1 influenza virus to high titres by van Wielink, R, Harmsen, M.M, Martens, D.E, Peeters, B.P.H, Wijffels, R.H, Moormann, R.J.M

“…Abstract Influenza A viruses lacking the gene encoding the non-structural NS1 protein (delNS1) have potential use as live attenuated vaccines. However, due to…”
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Passive immunization of pigs with bispecific llama single-domain antibody fragments against foot-and-mouth disease and porcine immunoglobulin by Harmsen, M.M., Fijten, H.P.D., Dekker, A., Eblé, P.L.

“…Foot-and-mouth disease (FMD) is a contagious viral disease of cloven-hoofed animals that occasionally causes outbreaks in Europe. We aim to develop an…”
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Passive immunization with llama single-domain antibody fragments reduces foot-and-mouth disease transmission between pigs by Harmsen, M.M, Fijten, H.P.D, Engel, B, Dekker, A, Eblé, P.L

“…Abstract We aim to develop a method that confers rapid protection against foot-and-mouth disease (FMD) by passive immunization with recombinant llama…”
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Passive immunization of guinea pigs with llama single-domain antibody fragments against foot-and-mouth disease by Harmsen, M.M., van Solt, C.B., Fijten, H.P.D., van Keulen, L., Rosalia, R.A., Weerdmeester, K., Cornelissen, A.H.M., De Bruin, M.G.M., Eblé, P.L., Dekker, A.

“…Foot-and-mouth disease (FMD) is a highly contagious disease that occasionally causes outbreaks in Europe. There is a need for therapies that provide rapid…”
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Effect of natural and chimeric haemagglutinin genes on influenza A virus replication in baby hamster kidney cells by van Wielink, R., Harmsen, M.M., Martens, D.E., de Leeuw, O.S., Peeters, B.P.H., Wijffels, R.H., Moormann, R.J.M.

“…► Poor replication of influenza PR8 strain on BHK21 cells is caused by the HA protein. ► Replication of several other strains on BHK21 cells also appears to be…”
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Characterization of foot-and-mouth disease virus antigen by surface-enhanced laser desorption ionization-time of flight-mass spectrometry in aqueous and oil-emulsion formulations by Harmsen, M.M, Jansen, J, Westra, D.F, Coco-Martin, J.M

“…Abstract We have used a novel method, surface-enhanced laser desorption ionization-time of flight-mass spectrometry (SELDI-TOF-MS), to characterize…”
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Escherichia coli F4 fimbriae specific llama single-domain antibody fragments effectively inhibit bacterial adhesion in vitro but poorly protect against diarrhoea by Harmsen, M.M., van Solt, C.B., Hoogendoorn, A., van Zijderveld, F.G., Niewold, T.A., van der Meulen, J.

“…Oral administration of polyclonal antibodies directed against enterotoxigenic Escherichia coli (ETEC) F4 fimbriae is used to protect against piglet…”
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Properties, production and applications of camelid single-domain antibody fragments by Harmsen, M.M, Haard, H.J. de

“…Camelids produce functional antibodies devoid of light chains of which the single N-terminal domain is fully capable of antigen binding. These single-domain…”
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The effect of uniform capture molecule orientation on biosensor sensitivity: Dependence on analyte properties by Trilling, Anke K., Harmsen, Michiel M., Ruigrok, Vincent J.B., Zuilhof, Han, Beekwilder, Jules

“…Uniform orientation of capture molecules on biosensors has been reported to increase sensitivity. Here it is investigated which analyte properties contribute…”
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Identification of the Structural Basis of Thermal Lability of a Virus Provides a Rationale for Improved Vaccines by Rincón, Verónica, Rodríguez-Huete, Alicia, López-Argüello, Silvia, Ibarra-Molero, Beatriz, Sanchez-Ruiz, Jose M., Harmsen, Michiel M., Mateu, Mauricio G.

“…Virus stability and dynamics play critical roles during infection. Some viruses, including foot-and-mouth disease virus (FMDV), are surprisingly prone to…”
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Characterization of epitope-tagged foot-and-mouth disease virus by SEAGO, Julian, JACKSON, Terry, DOEL, Claudia, FRY, Elizabeth, STUART, David, HARMSEN, Michiel M, CHARLESTON, Bryan, JULEFF, Nicholas

“…Foot-and-mouth disease (FMD) is a highly contagious and economically devastating disease of cloven-hoofed animals with an almost-worldwide distribution…”
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Selection and optimization of proteolytically stable llama single-domain antibody fragments for oral immunotherapy by HARMSEN, M. M, VAN SOFT, C. B, VAN ZIJDERVELD-VAN BEMMEL, A. M, NIEWOLD, T. A, VAN ZIJDERVELD, F. G

“…We previously demonstrated that oral application of the recombinant single-domain antibody fragment (VHH) clone K609, directed against Escherichia coli F4…”
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Effect of natural and chimeric haemagglutinin genes on influenza A virus replication in baby hamster kidney cells by Wielink, R., van, Harmsen, M.M, Martens, D.E, Leeuw, O.S., de, Peeters, B.P.H, Wijffels, R.H

“…Baby hamster kidney (BHK21) cells are used to produce vaccines against various viral veterinary diseases, including rabies and foot-and-mouth-disease. Although…”
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MDCK cell line with inducible allele B NS1 expression propagates deINS1 inflenza virus to high titres by Wielink, R., van, Harmsen, M.M, Martens, D.E, Peeters, B.P.H, Wijffels, R.H, Moormann, R.J.M

“…Influenza A viruses lacking the gene encoding the non-structural NS1 protein (delNS1) have potential use as live attenuated vaccines. However, due to the lack…”
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Enhancement of toxin- and virus-neutralizing capacity of single-domain antibody fragments by N-glycosylation by Harmsen, M. M, van Solt, C. B, Fijten, H. P. D

“…Single-domain antibody fragments (VHHs) have several beneficial properties as compared to conventional antibody fragments. However, their small size…”
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Prolonged in vivo residence times of llama single-domain antibody fragments in pigs by binding to porcine immunoglobulins by Harmsen, Michiel M., Van Solt, Conny B., Fijten, Helmi P.D., Van Setten, Marga C.

“…The therapeutic parenteral application of llama single-domain antibody fragments (VHHs) is hampered by their small size, resulting in a fast elimination from…”
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Mutations in the M-Gene Segment Can Substantially Increase Replication Efficiency of NS1 Deletion Influenza A Virus in MDCK Cells by van Wielink, R, Harmsen, M M, Martens, D E, Peeters, B P H, Wijffels, R H, Moormann, R J M

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