Search Results - "Harmer, Nicholas"

A Murder Mystery Gamification Session to Consolidate Analytical Biochemical Techniques Learning by Hill, Alison M., Harmer, Nicholas J.

“…Gamification has a strong track record of improving student engagement and learning in the chemical sciences. Meta-analyses of different approaches to…”
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Characterization of Carboxylic Acid Reductases as Enzymes in the Toolbox for Synthetic Chemistry by Finnigan, William, Thomas, Adam, Cromar, Holly, Gough, Ben, Snajdrova, Radka, Adams, Joseph P., Littlechild, Jennifer A., Harmer, Nicholas J.

“…Carboxylic acid reductase enzymes (CARs) meet the demand in synthetic chemistry for a green and regiospecific route to aldehydes from their respective…”
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Myosin-5, kinesin-1 and myosin-17 cooperate in secretion of fungal chitin synthase by Schuster, Martin, Treitschke, Steffi, Kilaru, Sreedhar, Molloy, Justin, Harmer, Nicholas J, Steinberg, Gero

“…Plant infection by pathogenic fungi requires polarized secretion of enzymes, but little is known about the delivery pathways. Here, we investigate the…”
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A half-site multimeric enzyme achieves its cooperativity without conformational changes by Vivoli, Mirella, Pang, Jiayun, Harmer, Nicholas J.

“…Cooperativity is a feature many multimeric proteins use to control activity. Here we show that the bacterial heptose isomerase GmhA displays homotropic…”
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Structural insights into the DNA recognition mechanism by the bacterial transcription factor PdxR by Freda, Ida, Exertier, Cécile, Barile, Anna, Chaves-Sanjuan, Antonio, Vega, Mirella Vivoli, Isupov, Michail N, Harmer, Nicholas J, Gugole, Elena, Swuec, Paolo, Bolognesi, Martino, Scipioni, Anita, Savino, Carmelinda, Di Salvo, Martino Luigi, Contestabile, Roberto, Vallone, Beatrice, Tramonti, Angela, Montemiglio, Linda Celeste

“…Abstract Specificity in protein–DNA recognition arises from the synergy of several factors that stem from the structural and chemical signatures encoded within…”
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Zoonoses under our noses by Cross, Alice R., Baldwin, Victoria M., Roy, Sumita, Essex-Lopresti, Angela E., Prior, Joann L., Harmer, Nicholas J.

“…One Health is an effective approach for the management of zoonotic disease in humans, animals and environments. Examples of the management of bacterial…”
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Drug screening to identify compounds to act as co-therapies for the treatment of Burkholderia species by Barker, Sam, Harding, Sarah V, Gray, David, Richards, Mark I, Atkins, Helen S, Harmer, Nicholas J

“…Burkholderia pseudomallei is a soil-dwelling organism present throughout the tropics. It is the causative agent of melioidosis, a disease that is believed to…”
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Using enzyme cascades in biocatalysis: Highlight on transaminases and carboxylic acid reductases by Cutlan, Rhys, De Rose, Simone, Isupov, Michail N., Littlechild, Jennifer A., Harmer, Nicholas J.

“…Biocatalysis, the use of enzymes in chemical transformations, is an important green chemistry tool. Cascade reactions combine different enzyme activities in a…”
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Molecular features of lipoprotein CD0873: A potential vaccine against the human pathogen Clostridioides difficile by Bradshaw, William J., Bruxelle, Jean-François, Kovacs-Simon, Andrea, Harmer, Nicholas J., Janoir, Claire, Péchiné, Severine, Acharya, K. Ravi, Michell, Stephen L.

“…Clostridioides difficile is the primary cause of antibiotic-associated diarrhea and colitis, a healthcare-associated intestinal disease resulting in a…”
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A universal fluorescence-based toolkit for real-time quantification of DNA and RNA nuclease activity by Sheppard, Emily C., Rogers, Sally, Harmer, Nicholas J., Chahwan, Richard

“…DNA and RNA nucleases play a critical role in a growing number of cellular processes ranging from DNA repair to immune surveillance. Nevertheless, many…”
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Engineering a Seven Enzyme Biotransformation using Mathematical Modelling and Characterized Enzyme Parts by Finnigan, William, Cutlan, Rhys, Snajdrova, Radka, Adams, Joseph P., Littlechild, Jennifer A., Harmer, Nicholas J.

“…Multi‐step enzyme reactions offer considerable cost and productivity benefits. Process models offer a route to understanding the complexity of these reactions,…”
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Woronin body-based sealing of septal pores by Steinberg, Gero, Harmer, Nicholas J., Schuster, Martin, Kilaru, Sreedhar

“…•Upon wounding, Woronin bodies seal hyphal septa in filamentous ascomycetes.•Little is known about the mechanism underpinning Woronin body…”
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Unique Data Sets and Bespoke Laboratory Videos: Teaching and Assessing of Experimental Methods and Data Analysis in a Pandemic by Harmer, Nicholas J, Hill, Alison M

“…The COVID-19 pandemic necessitated the move to online teaching and assessment. This has created challenges in teaching laboratory skills and producing…”
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The ROK kinase N-acetylglucosamine kinase uses a sequential random enzyme mechanism with successive conformational changes upon each substrate binding by Roy, Sumita, Vivoli Vega, Mirella, Ames, Jessica R., Britten, Nicole, Kent, Amy, Evans, Kim, Isupov, Michail N., Harmer, Nicholas J.

“…N-acetyl-d-glucosamine (GlcNAc) is a major component of bacterial cell walls. Many organisms recycle GlcNAc from the cell wall or metabolize environmental…”
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Determination of protein-ligand interactions using differential scanning fluorimetry by Vivoli, Mirella, Novak, Halina R, Littlechild, Jennifer A, Harmer, Nicholas J

“…A wide range of methods are currently available for determining the dissociation constant between a protein and interacting small molecules. However, most of…”
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Macrophage infectivity potentiator protein, a peptidyl prolyl cis-trans isomerase, essential for Coxiella burnetii growth and pathogenesis by Debowski, Aleksandra W, Bzdyl, Nicole M, Thomas, David R, Scott, Nichollas E, Jenkins, Christopher H, Iwasaki, Jua, Kibble, Emily A, Khoo, Chen Ai, Scheuplein, Nicolas J, Seibel, Pamela M, Lohr, Theresa, Metters, Georgie, Bond, Charles S, Norville, Isobel H, Stubbs, Keith A, Harmer, Nicholas J, Holzgrabe, Ulrike, Newton, Hayley J, Sarkar-Tyson, Mitali

“…Coxiella burnetii is a Gram-negative intracellular pathogen that causes the debilitating disease Q fever, which affects both animals and humans. The only…”
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The Structure of Sedoheptulose-7-Phosphate Isomerase from Burkholderia pseudomallei Reveals a Zinc Binding Site at the Heart of the Active Site by Harmer, Nicholas J.

“…Heptoses are found in the surface polysaccharides of most bacteria, contributing to structures that are essential for virulence and antibiotic resistance…”
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Effect of Substance P in Staphylococcus aureus and Staphylococcus epidermidis Virulence: Implication for Skin Homeostasis by N'Diaye, Awa, Mijouin, Lily, Hillion, Mélanie, Diaz, Suraya, Konto-Ghiorghi, Yoan, Percoco, Giuseppe, Chevalier, Sylvie, Lefeuvre, Luc, Harmer, Nicholas J, Lesouhaitier, Olivier, Feuilloley, Marc G J

“…Staphylococcus aureus and Staphylococcus epidermidis are two major skin associated bacteria, and Substance P (SP) is a major skin neuropeptide. Since bacteria…”
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Spinning sugars in antigen biosynthesis: characterization of the Coxiella burnetii and Streptomyces griseus TDP-sugar epimerases by Cross, Alice R., Roy, Sumita, Vivoli Vega, Mirella, Rejzek, Martin, Nepogodiev, Sergey A., Cliff, Matthew, Salmon, Debbie, Isupov, Michail N., Field, Robert A., Prior, Joann L., Harmer, Nicholas J.

“…The sugars streptose and dihydrohydroxystreptose (DHHS) are unique to the bacteria Streptomyces griseus and Coxiella burnetii, respectively. Streptose forms…”
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The HicA toxin from Burkholderia pseudomallei has a role in persister cell formation by Butt, Aaron, Higman, Victoria A, Williams, Christopher, Crump, Matthew P, Hemsley, Claudia M, Harmer, Nicholas, Titball, Richard W

“…TA (toxin-antitoxin) systems are widely distributed amongst bacteria and are associated with the formation of antibiotic tolerant (persister) cells that may…”
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