Search Results - "Harkins, Amy B"

Effects of Chemically Doped Bioactive Borate Glass on Neuron Regrowth and Regeneration by Gupta, Brinda, Papke, Jason B., Mohammadkhah, Ali, Day, Delbert E., Harkins, Amy B.

“…Peripheral nerve injuries present challenges to regeneration. Currently, the gold standard for nerve repair is an autograft that results in another region of…”
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Computational modeling of neurons: intensity-duration relationship of extracellular electrical stimulation for changes in intracellular calcium by Adams, Robert D, Willits, Rebecca K, Harkins, Amy B

“…In many instances of extensive nerve damage, the injured nerve never adequately heals, leaving lack of nerve function. Electrical stimulation (ES) has been…”
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PC12 Cells that Lack Synaptotagmin I Exhibit Loss of a Subpool of Small Dense Core Vesicles by Adams, Robert D., Harkins, Amy B.

“…Neurons communicate by releasing neurotransmitters that are stored in intracellular vesicular compartments. PC12 cells are frequently used as a model secretory…”
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Short-Term Electrical Stimulation to Promote Nerve Repair and Functional Recovery in a Rat Model by Calvey, Colleen, MD, Zhou, Wenda, MS, Stakleff, Kimberly Sloan, PhD, Sendelbach-Sloan, Patricia, BS, Harkins, Amy B., PhD, Lanzinger, William, MD, Willits, Rebecca Kuntz, PhD

“…Purpose To evaluate the effect of duration of electrical stimulation on peripheral nerve regeneration and functional recovery. Based on previous work, we…”
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Native and recombinant ASIC1a receptors conduct negligible Ca2+ entry by Samways, Damien S.K, Harkins, Amy B, Egan, Terrance M

“…Abstract Acid Sensing Ion Channels (ASICs) are a family of proton-gated cation channels that play a role in the sensation of noxious stimuli. Of these, ASIC1a…”
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Analysis of Amperometric Spike Shapes to Release Vesicles by Watson, Daniel J., Gummi, Rohit R., Papke, Jason B., Harkins , Amy B.

“…Carbon‐fiber amperometry is a real‐time analytical tool to detect vesicle release events from individual secretory cells. This study compared the spike events…”
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Validation of electrical stimulation models: intracellular calcium measurement in three-dimensional scaffolds by Adams, Robert D, Gupta, Brinda, Harkins, Amy B

“…Peripheral nerve injury can be disabling. Regeneration is limited by the rate of axonal extension, and proximal injury to peripheral nerves can take over a…”
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Stable gene silencing of synaptotagmin I in rat PC12 cells inhibits Ca2+-evoked release of catecholamine by Moore, Johnnie M, Papke, Jason B, Cahill, Anne L, Harkins, Amy B

“…Synaptotagmin (syt) I is a Ca2+-binding protein that is well accepted as a major sensor for Ca2+-regulated release of transmitter. However, controversy remains…”
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Upregulation of synaptotagmin IV inhibits transmitter release in PC12 cells with targeted synaptotagmin I knockdown by Moore-Dotson, Johnnie M, Papke, Jason B, Harkins, Amy B

“…The function of synaptotagmins (syt) in Ca2+-dependent transmitter release has been attributed primarily to Ca2+-dependent isoforms such as syt I. Recently,…”
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Deletion of the Synaptic Protein Interaction Site of the N-Type ( Ca V2.2) Calcium Channel Inhibits Secretion in Mouse Pheochromocytoma Cells by Harkins, Amy B., Cahill, Anne L., Powers, James F., Tischler, Arthur S., Fox, Aaron P., Catterall, William A.

“…Presynaptic N-type Ca2+ channels ( Ca v2.2, α 1 B) are thought to bind to SNARE (SNAP-25 receptor) complex proteins through a synaptic protein interaction…”
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Effects of borate-based bioactive glass on neuron viability and neurite extension by Marquardt, Laura M., Day, Delbert, Sakiyama-Elbert, Shelly E., Harkins, Amy B.

“…Bioactive glasses have recently been shown to promote regeneration of soft tissues by positively influencing tissue remodeling during wound healing. We were…”
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Stimulation Frequency Alters the Dorsal Root Ganglion Neurite Growth and Directionality In Vitro by Kumar, Pragya Jai, Adams, Robert D., Harkins, Amy B., Engeberg, Erik D., Willits, Rebecca Kuntz

“…Objective: To improve peripheral nerve repair, new techniques to increase the speed of regeneration are required. Studies have shown that the electrical…”
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Electrical and Neurotrophin Enhancement of Neurite Outgrowth Within a 3D Collagen Scaffold by Adams, Robert D., Rendell, Sara R., Counts, Lauren R., Papke, Jason B., Willits, Rebecca K., Harkins, Amy B.

“…Electrical and chemical stimulation have been studied as potent mechanisms of enhancing nerve regeneration and wound healing. However, it remains unclear how…”
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The impact of laminin on 3D neurite extension in collagen gels by Swindle-Reilly, Katelyn E, Papke, Jason B, Kutosky, Hannah P, Throm, Allison, Hammer, Joshua A, Harkins, Amy B, Willits, Rebecca Kuntz

“…The primary goal of this research was to characterize the effect of laminin on three-dimensional (3D) neurite growth. Gels were formed using type I collagen at…”
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Light Activation of Calcein Inhibits Vesicle Release of Catecholamines by Miller, Brooke A, Papke, Jason B, Bindokas, Vytas P, Harkins, Amy B

“…Calcein, a fluorescent fluid phase marker, has been used to track and visualize cellular processes such as synaptic vesicle fusion. It is also the fluorophore…”
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Activation of purinergic receptors by ATP inhibits secretion in bovine adrenal chromaffin cells by Harkins, Amy B., Fox, Aaron P.

“…Autoinhibition is a common mechanism observed in neurons to regulate neurotransmission. Released neurotransmitter interacts with presynaptic autoreceptors to…”
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Activation of nicotinic acetylcholine receptors augments calcium channel-mediated exocytosis in rat pheochromocytoma (PC12) cells by Harkins, A B, Fox, A P

“…The functional effect of activating Ca2+-permeable neuronal nicotinic acetylcholine receptors (nAChRs) on vesicle secretion was studied in PC12 cells. Single…”
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Stable RNA interference of synaptotagmin I in PC12 cells results in differential regulation of transmitter release by Roden, William H, Papke, Jason B, Moore, Johnnie M, Cahill, Anne L, Macarthur, Heather, Harkins, Amy B

“…In sympathetic neurons, it is well-established that the neurotransmitters, norepinephrine (NE), neuropeptide Y (NPY), and ATP are differentially coreleased…”
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Fabrication and characterization of poly-(ε)-caprolactone and bioactive glass composites for tissue engineering applications by Mohammadkhah, Ali, Marquardt, Laura M., Sakiyama-Elbert, Shelly E., Day, Delbert E., Harkins, Amy B.

“…Much work has focused on developing synthetic materials that have tailored degradation profiles and physical properties that may prove useful in developing…”
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Fabrication of amperometric electrodes by Pike, Carolyn M, Grabner, Chad P, Harkins, Amy B

“…Carbon fiber electrodes are crucial for the detection of catecholamine release from vesicles in single cells for amperometry measurements. Here, we describe…”
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