The potential for production of freeze-dried oral vaccines using alginate hydrogel microspheres as protein carriers

Oral administration of dry vaccine formulations is acknowledged to offer major clinical and logistical benefits by eliminating the cold chain required for liquid preparations. A model antigen, bovine serum albumin (BSA) was encapsulated in alginate microspheres using aerosolisation. Hydrated microsp...

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Bibliographic Details
Published in:	Journal of drug delivery science and technology Vol. 24; no. 2; pp. 178 - 184
Main Authors:	Hariyadi, D.M., Ma, Y., Wang, Y., Bostrom, T., Malouf, J., Turner, M.S., Bhandari, B., Coombes, A.G.A.
Format:	Journal Article
Language:	English
Published:	Elsevier B.V 01-01-2014
Subjects:	Alginate hydrogel microspheres Bovine serum albumin ESEM In vitro release Maltodextrin cryoprotectant Oral vaccine Protein Maltodextrin cryoprotectant Bovine serum albumin ESEM In vitro release Oral vaccine Alginate hydrogel microspheres Protein
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Summary:	Oral administration of dry vaccine formulations is acknowledged to offer major clinical and logistical benefits by eliminating the cold chain required for liquid preparations. A model antigen, bovine serum albumin (BSA) was encapsulated in alginate microspheres using aerosolisation. Hydrated microspheres 25 to 65 μm in size with protein loading of 3.3 % w/w were obtained. Environmental scanning electron microscopy indicated a stabilizing effect of encapsulated protein on alginate hydrogels revealed by an increase in dehydration resistance. Freeze drying of alginate microspheres without use of a cryoprotectant resulted in fragmentation and subsequent rapid loss of the majority of the protein load in simulated intestinal fluid in 2 h, whereas intact microspheres were observed following freeze-drying of BSA-loaded microspheres in the presence of maltodextrin. BSA release from freeze-dried preparations was limited to less than 7 % in simulated gastric fluid over 2 h, while 90 % of the protein load was gradually released in simulated intestinal fluid over 10 h. SDS-PAGE analysis indicated that released BSA largely preserved its molecular weight. These findings demonstrate the potential for manufacturing freeze-dried oral vaccines using alginate microspheres.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1773-2247
DOI:	10.1016/S1773-2247(14)50029-9