Induction of haploid rice plants through in vitro anther culture

Induction of haploid rice plants through in vitro anther culture

Anthers of three rice cultivars having late uninucleate microspores were studied for induction of haploid rice plants. Anthers were cultured on Z2 medium supplemented with various combinations and concentrations of auxins and kinetin (cytokinins). The best callusing from cultured anthers obtained in...

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Bibliographic Details
Published in:Pakistan journal of biological sciences Vol. 6; no. 14; pp. 1250 - 1252
Main Authors: Shahnewaz, S, Bari, M.A, Siddique, N.A, Khatun, N, Rahman, M.H, Haque, M.E. (Rajshahi Univ. (Bangladesh). Plant Biotechnology Lab.)
Format: Journal Article
Language:English
Published: 01-07-2003
Subjects:
2,4-D
ANA
ANTHER CULTURE
CITOQUININAS
CULTIVO DE ANTERAS
CULTURE D'ANTHÈRE
CULTURE MEDIA
CYTOKININE
CYTOKININS
HAPLOID
IN VITRO REGENERATION
MEDIO DE CULTIVO
MILIEU DE CULTURE
NAA
ORYZA SATIVA
REGENERACIÓN IN VITRO
RÉGÉNÉRATION IN VITRO
VARIEDADES
VARIETIES
VARIÉTÉ
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Summary:Anthers of three rice cultivars having late uninucleate microspores were studied for induction of haploid rice plants. Anthers were cultured on Z2 medium supplemented with various combinations and concentrations of auxins and kinetin (cytokinins). The best callusing from cultured anthers obtained in Z2 medium containing 2,4-D 2 mg L-1, NAA 2.5 mg L-1 and kin 0.5 mg L-1. For regeneration of haploid plantlets anther derived calli were transferred to modified MS medium enriched with kin 1 mg L-1, NAA 1 mg L-1, BAP 1.0 ml L-1 and incubated at 25 plus minus 1 degree centigrade in light. BRRI Dhan-29 produced both green and albino plants while BR-3 produced green plants only. Green plantlets obtained from BRRI Dhan-29 were examined and found to be 69.2% haploid plants.
Bibliography:2003000977
F02
ISSN:1028-8880
DOI:10.3923/pjbs.2003.1250.1252