Search Results - "Hanna, Ruth E."

Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities by Sanson, Kendall R., Hanna, Ruth E., Hegde, Mudra, Donovan, Katherine F., Strand, Christine, Sullender, Meagan E., Vaimberg, Emma W., Goodale, Amy, Root, David E., Piccioni, Federica, Doench, John G.

“…The creation of genome-wide libraries for CRISPR knockout (CRISPRko), interference (CRISPRi), and activation (CRISPRa) has enabled the systematic interrogation…”
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Genetic screens in isogenic mammalian cell lines without single cell cloning by DeWeirdt, Peter C., Sangree, Annabel K., Hanna, Ruth E., Sanson, Kendall R., Hegde, Mudra, Strand, Christine, Persky, Nicole S., Doench, John G.

“…Isogenic pairs of cell lines, which differ by a single genetic modification, are powerful tools for understanding gene function. Generating such pairs of…”
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Optimization of AsCas12a for combinatorial genetic screens in human cells by DeWeirdt, Peter C., Sanson, Kendall R., Sangree, Annabel K., Hegde, Mudra, Hanna, Ruth E., Feeley, Marissa N., Griffith, Audrey L., Teng, Teng, Borys, Samantha M., Strand, Christine, Joung, J. Keith, Kleinstiver, Benjamin P., Pan, Xuewen, Huang, Alan, Doench, John G.

“…Cas12a RNA-guided endonucleases are promising tools for multiplexed genetic perturbations because they can process multiple guide RNAs expressed as a single…”
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Benchmarking of SpCas9 variants enables deeper base editor screens of BRCA1 and BCL2 by Sangree, Annabel K., Griffith, Audrey L., Szegletes, Zsofia M., Roy, Priyanka, DeWeirdt, Peter C., Hegde, Mudra, McGee, Abby V., Hanna, Ruth E., Doench, John G.

“…Numerous rationally-designed and directed-evolution variants of SpCas9 have been reported to expand the utility of CRISPR technology. Here, we assess the…”
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Uncoupling of sgRNAs from their associated barcodes during PCR amplification of combinatorial CRISPR screens by Hegde, Mudra, Strand, Christine, Hanna, Ruth E, Doench, John G

“…Many implementations of pooled screens in mammalian cells rely on linking an element of interest to a barcode, with the latter subsequently quantitated by next…”
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CRISPR-Switch regulates sgRNA activity by Cre recombination for sequential editing of two loci by Chylinski, Krzysztof, Hubmann, Maria, Hanna, Ruth E., Yanchus, Connor, Michlits, Georg, Uijttewaal, Esther C. H., Doench, John, Schramek, Daniel, Elling, Ulrich

“…CRISPR-Cas9 is an efficient and versatile tool for genome engineering in many species. However, inducible CRISPR-Cas9 editing systems that regulate Cas9…”
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DYRK1A promotes viral entry of highly pathogenic human coronaviruses in a kinase-independent manner by Strine, Madison S, Cai, Wesley L, Wei, Jin, Alfajaro, Mia Madel, Filler, Renata B, Biering, Scott B, Sarnik, Sylvia, Chow, Ryan D, Patil, Ajinkya, Cervantes, Kasey S, Collings, Clayton K, DeWeirdt, Peter C, Hanna, Ruth E, Schofield, Kevin, Hulme, Christopher, Konermann, Silvana, Doench, John G, Hsu, Patrick D, Kadoch, Cigall, Yan, Qin, Wilen, Craig B

“…Identifying host genes essential for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has the potential to reveal novel drug targets and further…”
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Design and analysis of CRISPR–Cas experiments by Hanna, Ruth E., Doench, John G.

“…A large and ever-expanding set of CRISPR–Cas systems now enables the rapid and flexible manipulation of genomes in both targeted and large-scale experiments…”
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Massively parallel assessment of human variants with base editor screens by Hanna, Ruth E., Hegde, Mudra, Fagre, Christian R., DeWeirdt, Peter C., Sangree, Annabel K., Szegletes, Zsofia, Griffith, Audrey, Feeley, Marissa N., Sanson, Kendall R., Baidi, Yossef, Koblan, Luke W., Liu, David R., Neal, James T., Doench, John G.

“…Understanding the functional consequences of single-nucleotide variants is critical to uncovering the genetic underpinnings of diseases, but technologies to…”
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The Ankyrin Repeat Protein RARP-1 Is a Periplasmic Factor That Supports Rickettsia parkeri Growth and Host Cell Invasion by Sanderlin, Allen G, Hanna, Ruth E, Lamason, Rebecca L

“…spp. are obligate intracellular bacterial pathogens that have evolved a variety of strategies to exploit their host cell niche. However, the bacterial factors…”
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A case of mistaken identity by Hanna, Ruth E, Doench, John G

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Positive selection CRISPR screens reveal a druggable pocket in an oligosaccharyltransferase required for inflammatory signaling to NF-κB by Lampson, Benjamin L, Ramίrez, Ana S, Baro, Marta, He, Lixia, Hegde, Mudra, Koduri, Vidyasagar, Pfaff, Jamie L, Hanna, Ruth E, Kowal, Julia, Shirole, Nitin H, He, Yanfeng, Doench, John G, Contessa, Joseph N, Locher, Kaspar P, Kaelin, Jr, William G

“…Nuclear factor κB (NF-κB) plays roles in various diseases. Many inflammatory signals, such as circulating lipopolysaccharides (LPSs), activate NF-κB via…”
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Pharmacological disruption of mSWI/SNF complex activity restricts SARS-CoV-2 infection by Wei, Jin, Patil, Ajinkya, Collings, Clayton K., Alfajaro, Mia Madel, Liang, Yu, Cai, Wesley L., Strine, Madison S., Filler, Renata B., DeWeirdt, Peter C., Hanna, Ruth E., Menasche, Bridget L., Ökten, Arya, Peña-Hernández, Mario A., Klein, Jon, McNamara, Andrew, Rosales, Romel, McGovern, Briana L., Luis Rodriguez, M., García-Sastre, Adolfo, White, Kris M., Qin, Yiren, Doench, John G., Yan, Qin, Iwasaki, Akiko, Zwaka, Thomas P., Qi, Jun, Kadoch, Cigall, Wilen, Craig B.

“…Identification of host determinants of coronavirus infection informs mechanisms of viral pathogenesis and can provide new drug targets. Here we demonstrate…”
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Transforming Growth Factor Beta and Epithelial to Mesenchymal Transition Alter Homologous Recombination Repair Gene Expression and Sensitize BRCA Wild-Type Ovarian Cancer Cells to Olaparib by Roberts, Cai M, Rojas-Alexandre, Mehida, Hanna, Ruth E, Lin, Z Ping, Ratner, Elena S

“…Epithelial ovarian cancer (EOC) remains the most lethal gynecologic malignancy, largely due to metastasis and drug resistant recurrences. Fifteen percent of…”
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Genome-wide CRISPR Screens Reveal Host Factors Critical for SARS-CoV-2 Infection by Wei, Jin, Alfajaro, Mia Madel, DeWeirdt, Peter C., Hanna, Ruth E., Lu-Culligan, William J., Cai, Wesley L., Strine, Madison S., Zhang, Shang-Min, Graziano, Vincent R., Schmitz, Cameron O., Chen, Jennifer S., Mankowski, Madeleine C., Filler, Renata B., Ravindra, Neal G., Gasque, Victor, de Miguel, Fernando J., Patil, Ajinkya, Chen, Huacui, Oguntuyo, Kasopefoluwa Y., Abriola, Laura, Surovtseva, Yulia V., Orchard, Robert C., Lee, Benhur, Lindenbach, Brett D., Politi, Katerina, van Dijk, David, Kadoch, Cigall, Simon, Matthew D., Yan, Qin, Doench, John G., Wilen, Craig B.

“…Identification of host genes essential for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection may reveal novel therapeutic targets and…”
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