Cellular localization of dual positional specific maize lipoxygenase-1 in transgenic rice and calcium-mediated membrane association

The dual positional maize lipoxygenase-1 was introduced into rice and T2 transgenic plants were produced. Cellular location of maize lipoxygenase-1 in transgenic rice and effects of calcium ion on membrane association in vitro were analyzed. Localization study by confocal microscopic analysis indica...

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Published in:	Plant science (Limerick) Vol. 181; no. 3; pp. 242 - 248
Main Authors:	Cho, Kyoungwon, Han, Younsoo, Woo, Je Chang, Baudisch, Bianca, Klösgen, Ralf Bernd, Oh, Seunghan, Han, Jihoon, Han, Oksoo
Format:	Journal Article
Language:	English
Published:	Shannon Elsevier Ireland Ltd 01-09-2011 [Ireland]: Elsevier Science Ireland Ltd Elsevier
Subjects:	Agronomy. Soil science and plant productions binding sites Biological and medical sciences Calcium Calcium - metabolism Calcium-binding protein Carrier Proteins Chloroplasts Chloroplasts - chemistry Chloroplasts - enzymology Chloroplasts - metabolism corn Cytoplasm Cytoplasm - chemistry Cytoplasm - enzymology Cytoplasm - metabolism Dual positional specificity Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Plant Genes, Plant Genetic engineering applications Genetics and breeding of economic plants in vitro studies Lipoxygenase Lipoxygenase - chemistry Lipoxygenase - genetics Lipoxygenase - metabolism Maize Oryza - enzymology Oryza - genetics Oryza - metabolism Oryza sativa Plant breeding: fundamental aspects and methodology Plants, Genetically Modified - enzymology Plants, Genetically Modified - genetics Plants, Genetically Modified - metabolism Protein structure Protein Transport Rice Secondary structure Transgenic plants Translocation Zea mays Zea mays - enzymology Zea mays - genetics Zea mays - metabolism Dual positional specificity Translocation Maize Calcium Lipoxygenase Rice Monocotyledones Zea mays Enzyme Plant biology Cereal crop Oryza Gramineae Angiospermae Membrane Herbaceous plant Spermatophyta Transgenic plant Oxidoreductases
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Summary:	The dual positional maize lipoxygenase-1 was introduced into rice and T2 transgenic plants were produced. Cellular location of maize lipoxygenase-1 in transgenic rice and effects of calcium ion on membrane association in vitro were analyzed. Localization study by confocal microscopic analysis indicated that the maize lipoxygenase-1 was localized in cytoplasm. Sucrose-density fractionation experiment and in vitro protein transport to chloroplast showed that the maize lipoxygenase-1 can be associated with chloroplast. Secondary structure alignment revealed putative calcium binding sites in the PLAT domain of maize lipoxygenase-1 and the association of the maize lipoxygenase-1 with membranes was mediated by calcium ion in vitro. Our results provide evidences for calcium-mediated translocation of dual positional LOX without chloroplast targeting sequence from cytoplasm to chloroplast in plants for the first time.
Bibliography:	http://dx.doi.org/10.1016/j.plantsci.2011.05.016 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1
ISSN:	0168-9452 1873-2259
DOI:	10.1016/j.plantsci.2011.05.016