Genes induced during the early developmental stages of the Cane Toad, Bufo (Chaunus) marinus

Metamorphosis, a critical stage in the development of toads and frogs, involves rapid levels of morphological change. In the current study, we have used microarray analysis to identify shifts in gene expression between tadpole and toadlet stages of the cane toad, Bufo (Chaunus) marinus. Here, we rep...

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Bibliographic Details
Published in:	Gene Expression Patterns Vol. 8; no. 6; pp. 424 - 432
Main Authors:	Halliday, Damien C.T., Kennedy, Gavin C., Hamilton, Nicholas H.R., Tarmo, Suze, Alderman, James, Siddon, Nicole A., Robinson, Anthony J.
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01-07-2008
Subjects:	Actin Amino Acid Sequence Amphibia Amphibian Animals Anura Anuran Apolipoprotein Biocontrol Bufo Bufo marinus - embryology Bufo marinus - genetics Bufo marinus - growth & development Crystallin Gastrokine Gene expression Gene Expression Profiling Gene Expression Regulation, Developmental Haemoglobin Harderin Invasive Larva - genetics Larva - metabolism Larval Metamorphosis Microarray Molecular Sequence Data Olfactomedin Oligonucleotide Array Sequence Analysis RNA, Messenger - metabolism Sequence Homology, Amino Acid Serum albumin Tadpole Trefoil factor ORF Trefoil factor Microarray HDL Actin TFF RT-PCR Harderin dpf Metamorphosis aa Anuran Tadpole Haemoglobin Serum albumin Apolipoprotein Gene expression Biocontrol Gastrokine Crystallin Olfactomedin Larval vs Amphibian Invasive PCR
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Summary:	Metamorphosis, a critical stage in the development of toads and frogs, involves rapid levels of morphological change. In the current study, we have used microarray analysis to identify shifts in gene expression between tadpole and toadlet stages of the cane toad, Bufo (Chaunus) marinus. Here, we report on nine genes that show the greatest induction during metamorphosis; the gut-associated gastrokine and trefoil factor, blood components haemoglobins α/β, apolipoprotein and serum albumin, a nasal gene olfactomedin, a lens gene γ-crystallin, and a novel gene with low homology to frog harderin. We present both temporal and spatial expression patterns of these genes identified in developing and adult cane toads. This study extends our knowledge of the molecular basis of toad metamorphosis, and not only offers insights to the genes induced during the general remodelling that occurs but also reveals possible targets for control and manipulation of amphibian pest species, for example, the cane toad in Australia.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1567-133X 1872-7298
DOI:	10.1016/j.gep.2008.04.005