Search Results - "HamediRad, Mohammad"
-
1
Combinatorial metabolic engineering using an orthogonal tri-functional CRISPR system
Published in Nature communications (22-11-2017)“…Designing an optimal microbial cell factory often requires overexpression, knock-down, and knock-out of multiple gene targets. Unfortunately, such rewiring of…”
Get full text
Journal Article -
2
Multi-functional genome-wide CRISPR system for high throughput genotype–phenotype mapping
Published in Nature communications (19-12-2019)“…Genome-scale engineering is an indispensable tool to understand genome functions due to our limited knowledge of cellular networks. Unfortunately, most…”
Get full text
Journal Article -
3
Towards a fully automated algorithm driven platform for biosystems design
Published in Nature communications (13-11-2019)“…Large-scale data acquisition and analysis are often required in the successful implementation of the design, build, test, and learn (DBTL) cycle in biosystems…”
Get full text
Journal Article -
4
Directed evolution of a cellodextrin transporter for improved biofuel production under anaerobic conditions in Saccharomyces cerevisiae
Published in Biotechnology and bioengineering (01-08-2014)“…ABSTRACT Introduction of a cellobiose utilization pathway consisting of a cellodextrin transporter and a β‐glucosidase into Saccharomyces cerevisiae enables…”
Get full text
Journal Article -
5
CRISPR/Cas9-mediated knock-in of an optimized TetO repeat for live cell imaging of endogenous loci
Published in Nucleic acids research (28-09-2018)“…Abstract Nuclear organization has an important role in determining genome function; however, it is not clear how spatiotemporal organization of the genome…”
Get full text
Journal Article -
6
Comparison of muscle activation imbalance following core stability or general exercises in nonspecific low back pain: a quasi-randomized controlled trial
Published in BMC sports science, medicine & rehabilitation (15-04-2020)“…Low back pain causes changes in muscle activation patterns. Knowing how different exercises may improve altered muscle activation is useful in the treatment of…”
Get full text
Journal Article -
7
Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision
Published in Nature biotechnology (01-07-2018)“…Efficient genome-scale precision editing in yeast is enabled using CRISPR–Cas9 and homology-directed-repair. We developed a CRISPR–Cas9- and…”
Get full text
Journal Article -
8
Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System
Published in Biotechnology journal (01-09-2018)“…Thanks to its ease of use, modularity, and scalability, the clustered regularly interspaced short palindromic repeats (CRISPR) system has been increasingly…”
Get full text
Journal Article -
9
Regulatory RNA-assisted genome engineering in microorganisms
Published in Current opinion in biotechnology (01-12-2015)“…Graphical abstract…”
Get full text
Journal Article -
10
Multi-functional genome-wide CRISPR system for high throughput genotype–phenotype mapping
Published in Nature communications (19-12-2019)“…Abstract Genome-scale engineering is an indispensable tool to understand genome functions due to our limited knowledge of cellular networks. Unfortunately,…”
Get full text
Journal Article -
11
Highly Efficient Single-Pot Scarless Golden Gate Assembly
Published in ACS synthetic biology (17-05-2019)“…Golden Gate assembly is one of the most widely used DNA assembly methods due to its robustness and modularity. However, despite its popularity, the need for…”
Get full text
Journal Article -
12
RNAi assisted genome evolution unveils yeast mutants with improved xylose utilization
Published in Biotechnology and bioengineering (01-06-2018)“…Xylose is a major component of lignocellulosic biomass, one of the most abundant feedstocks for biofuel production. Therefore, efficient and rapid conversion…”
Get full text
Journal Article -
13
Towards a fully automated algorithm driven platform for biosystems design
Published in Nature communications (13-11-2019)“…Large-scale data acquisition and analysis are often required in the successful implementation of the design, build, test, and learn (DBTL) cycle in biosystems…”
Get full text
Journal Article -
14
Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision
Published in Nature biotechnology (07-05-2018)“…Here, we developed a CRISPR/Cas9 and homology-directed-repair assisted genome-scale engineering method named CHAnGE that can rapidly output tens of thousands…”
Get full text
Journal Article -
15
Orthogonal Fatty Acid Biosynthetic Pathway Improves Fatty Acid Ethyl Ester Production in Saccharomyces cerevisiae
Published in ACS synthetic biology (17-07-2015)“…Fatty acid ethyl esters (FAEEs) are a form of biodiesel that can be microbially produced via a transesterification reaction of fatty acids with ethanol. The…”
Get full text
Journal Article