β-glucan nanoparticles alleviate acute asthma by suppressing ferroptosis and DNA damage in mice

Asthma is a severe respiratory disease marked by airway inflammation, remodeling, and oxidative stress. β-Glucan (BG), a polysaccharide constituent of fungal cellular structures, exhibits potent immunomodulatory activities. The investigational focus was on the anti-asthmatic and anti-ferroptotic pro...

Full description

Saved in:

Bibliographic Details
Published in:	Apoptosis (London)
Main Authors:	Ebeed, Bassam W, Abdelmawgood, Islam Ahmed, Kotb, Mohamed A, Mahana, Noha A, Mohamed, Ayman Saber, Ramadan, Marwa A, Badr, Abeer Mahmoud, Nasr, Manar, Qurani, Osama Mohsen, Hamdy, Reem Mohamed, El-Hakiem, Nada Yasser Abd, Fahim, Mariam Khaled, Fekry, Mariam Morris, Eid, Jehane I
Format:	Journal Article
Language:	English
Published:	Netherlands 21-09-2024
Subjects:	Acute asthma Ovalbumin Oxidative stress Ferroptosis Inflammation Beta-glucan nanoparticles
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Asthma is a severe respiratory disease marked by airway inflammation, remodeling, and oxidative stress. β-Glucan (BG), a polysaccharide constituent of fungal cellular structures, exhibits potent immunomodulatory activities. The investigational focus was on the anti-asthmatic and anti-ferroptotic properties of beta-glucan nanoparticles (BG-NPs) in a murine model of allergic asthma induced by ovalbumin (OVA). BG was extracted from Chaga mushrooms (Inonotus obliquus), and its BG-NPs were characterized utilizing techniques including FT-IR, UV visible spectroscopy, zeta potential analysis, DLS, XRD, and TEM. The Balb/C mice were allocated into five groups: control, untreated asthmatic, dexamethasone (Dexa)-treated (1 mg/kg), BG-treated (100 mg/kg), BG-NPs-treated (45 mg/kg), and BG-treated (100 mg/kg). Treatment with BG-NPs markedly diminished the entry of inflammatory cells into the respiratory passage, serum IgE concentrations, DNA damage, and markers of oxidative stress through the reduction of malonaldehyde (MDA) levels and enhancing the levels of reduced glutathione (GSH), glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase (CAT). Furthermore, BG-NPs reduced iron deposition and promoted the transcriptional activity of the GPx4 gene in pulmonary cells, attenuating ferroptosis. The results demonstrated that BG-NPs reduced asthma by inhibiting oxidative stress, inflammation, DNA damage, and ferroptosis. Our results suggest that BG-NPs could be used as potential treatments for allergic asthma.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1360-8185 1573-675X 1573-675X
DOI:	10.1007/s10495-024-02013-9