international collaborative study to determine the prevalence of contagious caprine pleuropneumonia by monoclonal antibody-based cELISA

BACKGROUND: Few serological tests are available for detecting antibodies against Mycoplasma capricolum subsp. capripneumoniae, the causal agent of contagious caprine pleuropneumonia (CCPP). The complement fixation test, the test prescribed for international trade purposes, uses a crude antigen that...

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Published in:	BMC veterinary research Vol. 10; no. 1; p. 48
Main Authors:	Peyraud, Armelle, Poumarat, François, Tardy, Florence, Manso-Silván, Lucía, Hamroev, Karomatullo, Tilloev, Tillo, Amirbekov, Mullojon, Tounkara, Karim, Bodjo, Charles, Wesonga, Hezron, Nkando, Isabel Gacheri, Jenberie, Shiferaw, Yami, Martha, Cardinale, Eric, Meenowa, Deodass, Jaumally, Mahmad Reshad, Yaqub, Tahir, Shabbir, Muhammad Zubair, Mukhtar, Nadia, Halimi, Mohibullah, Ziay, Ghulam Mohammad, Schauwers, Willy, Noori, Hafizullah, Rajabi, Ali Madad, Ostrowski, Stéphane, Thiaucourt, François
Format:	Journal Article
Language:	English
Published:	England Springer-Verlag 24-02-2014 BioMed Central Ltd BioMed Central
Subjects:	Animal biology Animals antibodies Antibodies, Monoclonal antigens Bacterial Vaccines - immunology complement fixation tests contagious caprine pleuropneumonia economic impact enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods Enzyme-Linked Immunosorbent Assay - veterinary Global Health Goat Diseases - epidemiology Goat Diseases - microbiology Goats herds International trade Internationality Life Sciences Methods Mycoplasma capricolum Mycoplasma capricolum subsp. capripneumoniae Pleuropneumonia - epidemiology Pleuropneumonia - veterinary Pleuropneumonia, Contagious - epidemiology Pleuropneumonia, Contagious - microbiology Pleuropneumonia, Contagious - prevention & control seroconversion Seroepidemiologic Studies seroprevalence vaccination vaccines Veterinary medicine and animal Health Afghanistan Pakistan Kenya Mauritius Ethiopia Tajikistan France Global Health Enzyme-Linked Immunosorbent Assay Goats Contagious Antibodies Seroepidemiologic Studies Bacterial Vaccines Goat Diseases Mycoplasma capricolum Animals Internationality Monoclonal Pleuropneumonia
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Summary:	BACKGROUND: Few serological tests are available for detecting antibodies against Mycoplasma capricolum subsp. capripneumoniae, the causal agent of contagious caprine pleuropneumonia (CCPP). The complement fixation test, the test prescribed for international trade purposes, uses a crude antigen that cross-reacts with all the other mycoplasma species of the “mycoides cluster” frequently infecting goat herds. The lack of a more specific test has been a real obstacle to the evaluation of the prevalence and economic impact of CCPP worldwide. A new competitive ELISA kit for CCPP, based on a previous blocking ELISA, was formatted at CIRAD and used to evaluate the prevalence of CCPP in some regions of Kenya, Ethiopia, Mauritius, Tajikistan and Pakistan in an international collaborative study. RESULTS: The strict specificity of the test was confirmed in CCPP-free goat herds exposed to other mycoplasma species of the “mycoides cluster”. Prevalence studies were performed across the enzootic range of the disease in Africa and Asia. Seroprevalence was estimated at 14.6% in the Afar region of Ethiopia, whereas all the herds presented for CCPP vaccination in Kenya tested positive (individual seroprevalence varied from 6 to 90% within each herd). In Mauritius, where CCPP emerged in 2009, nine of 62 herds tested positive. In Central Asia, where the disease was confirmed only recently, no positive animals were detected in the Wakhan District of Afghanistan or across the border in neighboring areas of Tajikistan, whereas seroprevalence varied between 2.7% and 44.2% in the other districts investigated and in northern Pakistan. The test was also used to monitor seroconversion in vaccinated animals. CONCLUSIONS: This newly formatted CCPP cELISA kit has retained the high specificity of the original kit. It can therefore be used to evaluate the prevalence of CCPP in countries or regions without vaccination programs. It could also be used to monitor the efficacy of vaccination campaigns as high-quality vaccines induce high rates of seroconversion.
Bibliography:	http://dx.doi.org/10.1186/1746-6148-10-48 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1746-6148 1746-6148
DOI:	10.1186/1746-6148-10-48