Search Results - "Haley, B.E"

Identification of the uridine 5'-diphosphoglucose (UDP-Glc) binding subunit of cellulose synthase in Acetobacter xylinum using the photoaffinity probe 5-azido-UDP-Glc by Lin, F.C, Brown, R.M. Jr, Drake, R.R. Jr, Haley, B.E

“…Photoaffinity labeling of purified cellulose synthase with [beta-32P]5-azidouridine 5'-diphosphoglucose (UDP-Glc) has been used to identify the UDP-Glc binding…”
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Photoaffinity labeling of ribulose-1,5-bisphosphate carboxylase/oxygenase activase with ATP gamma-benzophenone. Identification of the ATP gamma-phosphate binding domain by Salvucci, M.E, Rajagopalan, K, Sievert, G, Haley, B.E, Watt, D.S

“…The phosphate-binding domain of the ATP-binding site of tobacco Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) activase was elucidated by…”
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Identification and characterization of a nucleotide binding site of ovine prolactin with 2-azido-NAD by Trad, C H, Chavan, A J, Clemens, J, Haley, B E

“…Photoaffinity labeling of ovine prolactin with the NAD+ photoaffinity analog [alpha-32P]nicotinamide-2-azidoadenine dinucleotide has been used to identify an…”
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Identification of the 64 kilodalton chloroplast stromal phosphoprotein as phosphoglucomutase by Salvucci, M.E. (USDA, ARS, Lexington, KY), Drake, R.R, Broadbent, K.P, Haley, B.E, Hanson, K.R, McHale, N.A

“…Phosphorylation of the 64 kilodalton stromal phosphoprotein by incubation of pea (Pisum sativum) chloroplast extracts with [gamma-32P]ATP decreased in the…”
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Identification of peptides within the base binding domains of the GTP- and ATP-specific binding sites of tubulin by JAYARAM, B, HALEY, B. E

“…Using gamma-32P-labeled 8-azidopurine nucleotide photoaffinity probes of GTP and ATP, the respective purine ring binding domain peptides of tubulin have been…”
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Identification of peptides from the adenine binding domains of ATP and AMP in adenylate kinase: isolation of photoaffinity-labeled peptides by metal chelate chromatography by Salvucci, Michael E, Chavan, Ashok J, Haley, Boyd E

“…Photoaffinity labeling with azidoadenine nucleotides was used to identify peptides from the ATP and AMP binding domains on chicken muscle adenylate kinase…”
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Photoaffinity labeling of ribulose-1,5-bisphosphate carboxylase/oxygenase activase wtih ATP gamma-benzophenone. Identification of the ATP gamma-phosphate binding domain by Salvucci, M.E, Rajagopalan, K, Sievert, G, Haley, B.E, Watt, D.S

“…The phosphate-binding domain of the ATP-binding site of tobacco Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) activase was elucidated by…”
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Formation of an intramolecular cystine disulfide during the reaction of 8-azidoguanosine 5 prime -triphosphate with cytosolic phosphoenolpyruvate carboxykinase (GTP) causes inactivation without photolabeling by Lewis, C.T., Carlson, G.M., Haley, B.E.

“…Phosphoenolpyruvate carboxykinase (GTP) (PEPCK) specifically utilizes a guanosine or inosine nucleotide as a substrate, yet it does not share extended sequence…”
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HgEDTA complex inhibits GTP interactions with the E-site of brain beta-tubulin by Duhr, E F, Pendergrass, J C, Slevin, J T, Haley, B E

“…We have found that EDTA and EGTA complexes of Hg2+, which conventional wisdom has assumed are biologically inert, are potentially injurious to the neuronal…”
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Synthesis and application of bidentate photoaffinity cross-linking reagents. Nucleotide photoaffinity probes with two photoactive groups by RAJAGOPALAN, K, CHAVAN, A. J, HALEY, B. E, WATT, D. S

“…Two "targeted bidentate" photoaffinity cross-linking reagents, the monoanhydride of 8-N3ADP with N-(4-(benzoyl)phenylmethyl)phosphoramide ([gamma-32P]8-N3ATP…”
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NAD+ binding site of Clostridium botulinum C3 ADP-ribosyltransferase. Identification of peptide in the adenine ring binding domain using 2-azido NAD by CHAVAN, A. J, NEMOTO, Y, NARUMIYA, S, KOZAKI, S, HALEY, B. E

“…C3 ADP-ribosyltransferase is an exoenzyme produced by certain strains of Clostridium botulinum types C and D, which specifically ADP-ribosylates rho proteins…”
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Synthesis and biological properties of 5-azido-2'-deoxyuridine 5'-triphosphate, a photoactive nucleotide suitable for making light-sensitive DNA by Evans, Robert K, Haley, Boyd E

“…A photoactive nucleotide analogue of dUTP, 5-azido-2'-deoxyuridine 5'-triphosphate (5-N3dUTP), was synthesized from dUMP in five steps. The key reaction in the…”
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Synthesis and properties of 2-azido-NAD+. A study of interaction with glutamate dehydrogenase by Kim, H, Haley, B E

“…A photoactive coenzyme analog of NAD+ has been synthesized by chemically coupling [32P]2-azido-AMP and NMN to produce [32P]nicotinamide 2-azidoadenosine…”
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Photoaffinity labeling of the rabbit reticulocyte guanine nucleotide exchange factor and eukaryotic initiation factor 2 with 8-azidopurine nucleotides by Dholakia, J.N., Francis, B.R., Haley, B.E., Wahba, A.J.

“…We have covalently modified rabbit reticulocyte polypeptide chain initiation factor 2 (eIF-2) and the guanine nucleotide exchange factor (GEF) with the 8-azido…”
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Photoaffinity labeling of human placental NAD(+)-linked 15-hydroxyprostaglandin dehydrogenase with [alpha-32P]2N3NAD+. Identification of a peptide in the adenine ring binding domain by Chavan, A J, Ensor, C M, Wu, P, Haley, B E, Tai, H H

“…Oxidation of many prostaglandins at C-15 results in the formation of 15-keto metabolites, which have reduced biological activity. This reaction is catalyzed by…”
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Identification of the UDP-glucose-binding polypeptide of callose synthase from Beta vulgaris L. by photoaffinity labeling with 5-azido-UDP-glucose by Frost, D.J. (Cook College, Rutgers University, New Brunswick, NJ), Read, S.M, Drake, R.R, Haley, B.E, Wasserman, B.P

“…The photoaffinity probe 5-azidouridine 5'-[beta-32P]diphosphate glucose (5N3[32P]UDP-Glc) was used to identify a 57-kDa polypeptide as a strong candidate for…”
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Photoaffinity labeling of a viral induced protein from tobacco by Evans, R.K, Haley, B.E, Roth, D.A

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Ischemic brain injury selectively alters ATP binding of calcium and calmodulin-dependent protein kinase II by Churn, S B, Sankaran, B, Haley, B E, DeLorenzo, R J

“…Calmodulin and ATP affinity and total binding capacity were characterized for CaM kinase II isolated from control and ischemic animals. Ischemic CaM kinase II…”
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Synthesis and characterization of 5-azido-UDP-glucuronic acid. A new photoaffinity probe for UDP-glucuronic acid-utilizing proteins by DRAKE, R. R, ZIMNIAK, P, HALEY, B. E, LESTER, R, ELBEIN, A. D, RADOMINSKA, A

“…A new active site-directed photoaffinity analogue, [beta-32P]5-azido-UDP-glucuronic acid (UDP-GlcA), was enzymatically synthesized from [beta-32P]5-N3UDP-Glc…”
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Purification and photoaffinity labeling of sucrose phosphate synthase from spinach leaves by Salvucci, M E, Drake, R R, Haley, B E

“…Sucrose phosphate synthase (SPS) was isolated from spinach leaves by precipitation with polyethylene glycol, ion-exchange and hydrophobic interaction…”
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