In silico Characterization of Plant and Microbial Antifreeze Proteins

Antifreeze proteins (AFPs) are class of proteins that protect organisms from the damage caused by freezing through their ability to inhibit ice growth and effectively lower the temperature at which water freezes. In this study, a total of 25 antifreeze proteins were selected from four different sour...

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Bibliographic Details
Published in:	Bioautomation Vol. 16; no. 4; pp. 225 - 238
Main Authors:	Hossain, Md Musharaf, Abu Taher Mohammad Jannatul Mosnaz, Abdul Mohin Sajib, Halder, Jewel, Mahmud, Raihan, Khairul Alam Mohammad Nayeem, Nabangshu Shekhar Das, Muzahidul Islam Sarkar, Dey, Janmajoy, Nahar, Akhikun
Format:	Journal Article
Language:	English
Published:	Sophia Bulgarska Akademiya na Naukite / Bulgarian Academy of Sciences 2012 Bulgarian Academy of Sciences
Subjects:	Antifreeze Proteins Antifreeze solutions Homology modelling Hydrophobicity Ice In silico analysis Isoelectric point Proteins
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Summary:	Antifreeze proteins (AFPs) are class of proteins that protect organisms from the damage caused by freezing through their ability to inhibit ice growth and effectively lower the temperature at which water freezes. In this study, a total of 25 antifreeze proteins were selected from four different sources (plant, bacteria and fungus) where they represent distinct physicochemical and structural features. Several Physico-chemical properties such as grand average hydropathy (GRAVY), aliphatic index (AI), extinction coefficient (EC), isolelectric point (pI), and instability index (II) were computed. S-S bridges and secondary structures were analyzed using CYS_REC and SOPMA programs respectively. The three dimensional structure of Antifreeze proteins is predicted by using three homology modelling server Geno3D, Swiss-model and CPHmodels. These models were evaluated with PROCHECK, What If, and ProSA programs. Model visualization and analysis was done with Pymol. These structures will provide a good foundation for functional analysis of experimentally derived crystal structures.
ISSN:	1314-1902 1314-2321