Activity-directed fractionation and isolation of four antibacterial compounds from Abrus precatorius L. roots

Activity-directed fractionation and isolation of four antibacterial compounds from Abrus precatorius L. roots

Root extracts of the plant Abrus precatorius L. was tested for antibacterial activity. Various solvent fractions exhibited inhibitory activity against 13 gram-positive and gram-negative bacteria. Root extracts were analyzed by thin layer chromatography. The antibacterial activity was localized to sp...

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Bibliographic Details
Published in:Natural product research Vol. 21; no. 10; pp. 933 - 940
Main Authors: Zore, GB, Awad, V, Thakre, AD, Halde, UK, Meshram, NS, Surwase, BS, Mohan Karuppayil, S
Format: Journal Article
Language:English
Published: England Taylor & Francis Group 01-08-2007
Subjects:
Abrus
Abrus precatorius
Abrus precatorius L
Anti-Bacterial Agents - administration & dosage
Anti-Bacterial Agents - chemistry
Anti-Bacterial Agents - pharmacology
Anti-Bacterial Agents - therapeutic use
Antibacterial
Antibiotics
Bioautography
Fabaceae
Humans
Indian licorice
Microbial Sensitivity Tests
Phytotherapy
Plant Extracts - administration & dosage
Plant Extracts - chemistry
Plant Extracts - pharmacology
Plant Extracts - therapeutic use
Plant Roots
Staphylococcus aureus
Staphylococcus aureus - drug effects
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Summary:Root extracts of the plant Abrus precatorius L. was tested for antibacterial activity. Various solvent fractions exhibited inhibitory activity against 13 gram-positive and gram-negative bacteria. Root extracts were analyzed by thin layer chromatography. The antibacterial activity was localized to specific chromatophores in the chloroform fraction through a bioautography assay. It was found localized to 4 chromatophores out of 7. The chromatophores were isolated from the TLC plates and rechecked for activity against Staphylococcus aureus A, using a disc diffusion assay. Among the four active principles isolated, AP 3 (Rf 0.87) exhibited maximum activity, i.e., 56% inhibition of growth of S. aureus A, in disc diffusion assay compared to the standard antibiotic Ampicillin. Results of this study suggest that chloroform extractable phytochemicals in A. precatorius L. may yield promising molecules with antibiotic activity.
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ISSN:1478-6419
1478-6427
DOI:10.1080/14786410701525992