High level fructan accumulation in a transgenic sugar beet

We have transformed sugar beet into a crop that produces fructans. The gene encoding 1-sucrose:sucrose fructosyl transferase (1-SST), which was isolated from Helianthus tuberosus, was introduced into sugar beet. In H. tuberosus, 1-SST mediates the first steps in fructan synthesis through the convers...

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Bibliographic Details
Published in:	Nature biotechnology Vol. 16; no. 9; pp. 843 - 846
Main Authors:	Sevenier, R. (CPRO-DLO, Wageningen, The Netherlands.), Hall, R.D, Meer, I.M. van der, Hakkert, H.J.C, Tunen, A.J. van, Koops, A.J
Format:	Journal Article
Language:	English
Published:	New York, NY Nature 01-09-1998
Subjects:	1-SST GENE 1-SUCROSE:SUCROSE FRUCTOSYL TRANSFERASE BETA VULGARIS BETA VULGARIS VAR. SACCHARIFERA Biological and medical sciences Biotechnology Carbohydrates - analysis Chenopodiaceae - genetics Chenopodiaceae - metabolism Chromatography, High Pressure Liquid Chromatography, Ion Exchange Chromatography, Thin Layer Fructans - biosynthesis Fructans - metabolism Fundamental and applied biological sciences. Psychology GENE GENES Genetic engineering Genetic technics GLICOSILTRANSFERASAS GLYCOSYLTRANSFERASE GLYCOSYLTRANSFERASES HELIANTHUS TUBEROSUS HEXOSYLTRANSFERASES Hexosyltransferases - genetics Methods. Procedures. Technologies Plant Proteins PLANTAS TRANSGENICAS PLANTE TRANSGENIQUE Plants, Genetically Modified Transgenic animals and transgenic plants TRANSGENIC PLANTS Genetic transformation Enzyme Transferases Glycosyltransferases Compositae Beta vulgaris Gene expression Sugar plant fructosyltransferase Chenopodiaceae Helianthus tuberosus Dicotyledones Angiospermae Fructosan Hexosyltransferases Spermatophyta Carbohydrate Transgenic plant Biological accumulation Sucrose 1
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Summary:	We have transformed sugar beet into a crop that produces fructans. The gene encoding 1-sucrose:sucrose fructosyl transferase (1-SST), which was isolated from Helianthus tuberosus, was introduced into sugar beet. In H. tuberosus, 1-SST mediates the first steps in fructan synthesis through the conversion of sucrose (GF) into low molecular weight fructans GF2, GF3, and GF4. In the taproot of sugar beet transformed with the 1-sst gene, the stored sucrose is almost totally converted into low molecular weight fructans. In contrast, 1-sst expression in the leaves resulted in only low levels of fructans. Despite the storage carbohydrate having been altered, the expression of the 1-sst gene did not have any visible effect on phenotype and did not affect the growth rate of the taproot as observed under greenhouse conditions.
Bibliography:	1997078905 F60 F30 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	1087-0156 1546-1696
DOI:	10.1038/nbt0998-843