GLI Transcriptional Targets S100A7 and KRT16 Show Upregulated Expression Patterns in Epidermis Overlying the Tumor Mass in Melanoma Samples

Although not completely understood, the role of the Hedgehog-GLI (HH-GLI) signaling pathway in melanoma and epithelial skin tumors has been reported before. In this study, we confirmed in various melanoma cell line models that keratin 16 (KRT16) and S100 Calcium-Binding Protein A7 (S100A7) are trans...

Full description

Saved in:

Bibliographic Details
Published in:	International journal of molecular sciences Vol. 25; no. 11; p. 6084
Main Authors:	Kurtović, Matea, Piteša, Nikolina, Čonkaš, Josipa, Hajpek, Helena, Vučić, Majda, Musani, Vesna, Ozretić, Petar, Sabol, Maja
Format:	Journal Article
Language:	English
Published:	Switzerland MDPI AG 01-06-2024 MDPI
Subjects:	Aged Analysis biomarkers Biomarkers, Tumor - genetics Biomarkers, Tumor - metabolism Cell growth Cell Line, Tumor Cytokines Development and progression Disease DNA binding proteins epidermis Epidermis - metabolism Epidermis - pathology Female Gene expression Gene Expression Regulation, Neoplastic Genetic aspects Genetic transcription HH-GLI Humans Keratin Keratin-16 - genetics Keratin-16 - metabolism keratins Male Melanoma Melanoma - genetics Melanoma - metabolism Melanoma - pathology Metastasis Middle Aged Protein binding Proteins Psoriasis S100 Calcium Binding Protein A7 - genetics S100 Calcium Binding Protein A7 - metabolism S100A7 Skin Skin cancer Skin Neoplasms - genetics Skin Neoplasms - metabolism Skin Neoplasms - pathology Tumor staging Up-Regulation Zinc Finger Protein GLI1 - genetics Zinc Finger Protein GLI1 - metabolism Germany California Croatia melanoma keratins biomarkers HH-GLI S100A7 epidermis
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Although not completely understood, the role of the Hedgehog-GLI (HH-GLI) signaling pathway in melanoma and epithelial skin tumors has been reported before. In this study, we confirmed in various melanoma cell line models that keratin 16 (KRT16) and S100 Calcium-Binding Protein A7 (S100A7) are transcriptional targets of GLI Family Zinc Finger (GLI) proteins. Besides their important role in protecting and maintaining the epidermal barrier, keratins are somehow tightly connected with the S100 family of proteins. We found that stronger expression of KRT16 indeed corresponds to stronger expression of S100A7 in our clinical melanoma samples. We also report a trend regarding staining of GLI1, which corresponds to stronger staining of GLI3, KRT16, and S100A7 proteins. The most interesting of our findings is that all the proteins are detected specifically in the epidermis overlying the tumor, but rarely in the tumor itself. The examined proteins were also not detected in the healthy epidermis at the edges of the sample, suggesting that the staining is specific to the epidermis overlaying the tumor mass. Of all proteins, only S100A7 demonstrated a statistically significant trend regarding tumor staging and staining intensity. Results from our clinical samples prove that immune infiltration is an important feature of melanoma. Pigmentophages and tumor-infiltrating lymphocytes (TIL) demonstrate a significant association with tumor stage, while mononuclear cells are equally present in all stages. For S100A7, we found an association between the number of TILs and staining intensity. Considering these new findings presented in our study, we suggest a more detailed examination of the possible role of the S100A7 protein as a biomarker in melanoma.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1422-0067 1661-6596 1422-0067
DOI:	10.3390/ijms25116084