Search Results - "Hahn, Klaus M."

Deep learning enables structured illumination microscopy with low light levels and enhanced speed by Jin, Luhong, Liu, Bei, Zhao, Fenqiang, Hahn, Stephen, Dong, Bowei, Song, Ruiyan, Elston, Timothy C., Xu, Yingke, Hahn, Klaus M.

“…Structured illumination microscopy (SIM) surpasses the optical diffraction limit and offers a two-fold enhancement in resolution over diffraction limited…”
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Optogenetic approaches to cell migration and beyond by Weitzman, Matthew, Hahn, Klaus M

“…Optogenetics, the use of genetically encoded tools to control protein function with light, can generate localized changes in signaling within living cells and…”
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Engineering extrinsic disorder to control protein activity in living cells by Dagliyan, Onur, Tarnawski, Miroslaw, Chu, Pei-Hsuan, Shirvanyants, David, Schlichting, Ilme, Dokholyan, Nikolay V., Hahn, Klaus M.

“…Optogenetic and chemogenetic control of proteins has revealed otherwise inaccessible facets of signaling dynamics. Here, we use light- or ligand-sensitive…”
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Profiling cellular morphodynamics by spatiotemporal spectrum decomposition by Ma, Xiao, Dagliyan, Onur, Hahn, Klaus M, Danuser, Gaudenz

“…Cellular morphology and associated morphodynamics are widely used for qualitative and quantitative assessments of cell state. Here we implement a framework to…”
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A genetically encoded photoactivatable Rac controls the motility of living cells by Wu, Yi I, Hahn, Klaus M, Frey, Daniel, Lungu, Oana I, Jaehrig, Angelika, Schlichting, Ilme, Kuhlman, Brian

“…The precise spatio-temporal dynamics of protein activity are often critical in determining cell behaviour, yet for most proteins they remain poorly understood;…”
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Amiloride inhibits macropinocytosis by lowering submembranous pH and preventing Rac1 and Cdc42 signaling by Koivusalo, Mirkka, Welch, Christopher, Hayashi, Hisayoshi, Scott, Cameron C, Kim, Moshe, Alexander, Todd, Touret, Nicolas, Hahn, Klaus M, Grinstein, Sergio

“…Macropinocytosis is differentiated from other types of endocytosis by its unique susceptibility to inhibitors of Na⁺/H⁺ exchange. Yet, the functional…”
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An Orally Bioavailable Chemical Probe of the Lysine Methyltransferases EZH2 and EZH1 by Konze, Kyle D, Ma, Anqi, Li, Fengling, Barsyte-Lovejoy, Dalia, Parton, Trevor, MacNevin, Christopher J, Liu, Feng, Gao, Cen, Huang, Xi-Ping, Kuznetsova, Ekaterina, Rougie, Marie, Jiang, Alice, Pattenden, Samantha G, Norris, Jacqueline L, James, Lindsey I, Roth, Bryan L, Brown, Peter J, Frye, Stephen V, Arrowsmith, Cheryl H, Hahn, Klaus M, Wang, Gang Greg, Vedadi, Masoud, Jin, Jian

“…EZH2 or EZH1 is the catalytic subunit of the polycomb repressive complex 2 that catalyzes methylation of histone H3 lysine 27 (H3K27). The trimethylation of…”
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Coordination of Rho GTPase activities during cell protrusion by Welch, Christopher, Elliott, Hunter, Johnson, Gary L, Nalbant, Perihan, Hodgson, Louis, Pertz, Olivier, Danuser, Gaudenz, Abell, Amy, Hahn, Klaus M, Machacek, Matthias

“…The GTPases Rac1, RhoA and Cdc42 act together to control cytoskeleton dynamics. Recent biosensor studies have shown that all three GTPases are activated at the…”
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Local control of intracellular microtubule dynamics by EB1 photodissociation by van Haren, Jeffrey, Charafeddine, Rabab A., Ettinger, Andreas, Wang, Hui, Hahn, Klaus M., Wittmann, Torsten

“…End-binding proteins (EBs) are adaptors that recruit functionally diverse microtubule plus-end-tracking proteins (+TIPs) to growing microtubule plus ends. To…”
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Light-mediated activation reveals a key role for Rac in collective guidance of cell movement in vivo by He, Li, Hahn, Klaus M, Montell, Denise J, Wang, Xiaobo, Wu, Yi I

“…The small GTPase Rac induces actin polymerization, membrane ruffling and focal contact formation in cultured single cells but can either repress or stimulate…”
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Computational design of chemogenetic and optogenetic split proteins by Dagliyan, Onur, Krokhotin, Andrey, Ozkan-Dagliyan, Irem, Deiters, Alexander, Der, Channing J., Hahn, Klaus M., Dokholyan, Nikolay V.

“…Controlling protein activity with chemogenetics and optogenetics has proven to be powerful for testing hypotheses regarding protein function in rapid…”
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Spatial models of pattern formation during phagocytosis by Herron, John Cody, Hu, Shiqiong, Liu, Bei, Watanabe, Takashi, Hahn, Klaus M, Elston, Timothy C

“…Phagocytosis, the biological process in which cells ingest large particles such as bacteria, is a key component of the innate immune response. Fcγ receptor…”
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High-resolution quantification of focal adhesion spatiotemporal dynamics in living cells by Berginski, Mathew E, Vitriol, Eric A, Hahn, Klaus M, Gomez, Shawn M

“…Focal adhesions (FAs) are macromolecular complexes that provide a linkage between the cell and its external environment. In a motile cell, focal adhesions…”
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Spatiotemporal dynamics of RhoA activity in migrating cells by Pertz, Olivier, Hahn, Klaus M, Hodgson, Louis, Klemke, Richard L

“…Rho family GTPases regulate the actin and adhesion dynamics that control cell migration. Current models postulate that Rac promotes membrane protrusion at the…”
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Differential Regulation of Protrusion and Polarity by PI(3)K during Neutrophil Motility in Live Zebrafish by Yoo, Sa Kan, Deng, Qing, Cavnar, Peter J., Wu, Yi I., Hahn, Klaus M., Huttenlocher, Anna

“…Cell polarity is crucial for directed migration. Here we show that phosphoinositide 3-kinase (PI(3)K) mediates neutrophil migration in vivo by differentially…”
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Engineered allosteric activation of kinases in living cells by Dokholyan, Nikolay V, Hahn, Klaus M, Karginov, Andrei V, Ding, Feng, Kota, Pradeep

“…Studies of cellular and tissue dynamics benefit greatly from tools that can control protein activity with specificity and precise timing in living systems…”
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Discovery of long-range inhibitory signaling to ensure single axon formation by Takano, Tetsuya, Wu, Mengya, Nakamuta, Shinichi, Naoki, Honda, Ishizawa, Naruki, Namba, Takashi, Watanabe, Takashi, Xu, Chundi, Hamaguchi, Tomonari, Yura, Yoshimitsu, Amano, Mutsuki, Hahn, Klaus M., Kaibuchi, Kozo

“…A long-standing question in neurodevelopment is how neurons develop a single axon and multiple dendrites from common immature neurites. Long-range inhibitory…”
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Spatiotemporal dynamics of GEF-H1 activation controlled by microtubule- and Src-mediated pathways by Azoitei, Mihai L, Noh, Jungsik, Marston, Daniel J, Roudot, Philippe, Marshall, Christopher B, Daugird, Timothy A, Lisanza, Sidney L, Sandí, María-José, Ikura, Mitsu, Sondek, John, Rottapel, Robert, Hahn, Klaus M, Danuser, Gaudenz

“…Rho family GTPases are activated with precise spatiotemporal control by guanine nucleotide exchange factors (GEFs). Guanine exchange factor H1 (GEF-H1), a RhoA…”
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Actin nano-architecture of phagocytic podosomes by Herron, J. Cody, Hu, Shiqiong, Watanabe, Takashi, Nogueira, Ana T., Liu, Bei, Kern, Megan E., Aaron, Jesse, Taylor, Aaron, Pablo, Michael, Chew, Teng-Leong, Elston, Timothy C., Hahn, Klaus M.

“…Podosomes are actin-enriched adhesion structures important for multiple cellular processes, including migration, bone remodeling, and phagocytosis. Here, we…”
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Millisecond spatiotemporal dynamics of FRET biosensors by the pair correlation function and the phasor approach to FLIM by Hinde, Elizabeth, Digman, Michelle A., Hahn, Klaus M., Gratton, Enrico

“…Here we present a fluctuation-based approach to biosensor Förster resonance energy transfer (FRET) detection that can measure the molecular flow and signaling…”
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