Search Results - "Hachmann, John P."

Models of protein modification in Tris–glycine and neutral pH Bis–Tris gels during electrophoresis: Effect of gel pH by Hachmann, John P., Amshey, Joseph W.

“…The pH of conventional Tris–glycine SDS–PAGE gels during a run is determined to be 9.5, in contrast to Bis–Tris–Mes gels where the pH is 7.2. Concentrations of…”
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A Multiplexed Protein Kinase Assay by Shults, Melissa D., Kozlov, Igor A., Nelson, Nicholas, Kermani, Bahram G., Melnyk, Peter C., Shevchenko, Veronika, Srinivasan, Anu, Musmacker, Joseph, Hachmann, John P., Barker, David L., Lebl, Michal, Zhao, Chanfeng

“…We report a novel protein kinase assay designed for high‐throughput detection of one or many kinases in a complex mixture. A solution‐phase phosphorylation…”
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A high-complexity, multiplexed solution-phase assay for profiling protease activity on microarrays by Kozlov, Igor A, Melnyk, Peter C, Hachmann, John P, Srinivasan, Anu, Shults, Melissa, Zhao, Chanfeng, Musmacker, Joseph, Nelson, Nicholas, Barker, David L, Lebl, Michal

“…We have developed a miniaturized and multiplexed solution assay for the measurement of protease activity in complex samples. This technology can accelerate…”
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Evaluation of Different Chemical Strategies for Conjugation of Oligonucleotides to Peptides by Kozlov, Igor A., Melnyk, Peter C., Hachmann, John P., Barker, David L., Lebl, Michal, Zhao, Chanfeng

“…We developed novel assays for high-throughput detection of one or many kinases or proteases. The assays use hundreds of different peptide substrates, each…”
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Synthesis of Poly d(G-C) Oligonucleotides by Hachmann, John P., Lebl, Michal

“…Model sequences for evaluation of the GC dimer sequence repetition on synthesis success were prepared and analyzed by HPLC. Contiguous d(G-C) or d(C-G)…”
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Retention of Histidine-Containing Peptides on a Nickel Affinity Column by Kozlov, Igor A., Kermani, Bahram G., Melnyk, Peter C., Barker, David L., Zhao, Chanfeng, Hachmann, John P., Lebl, Michal

“…The retention of histidine-containing peptides in immobilized metal-affinity chromatography is studied using several hundred modeled peptides. Retention is…”
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A method for rapid protease substrate evaluation and optimization by Kozlov, Igor A, Melnyk, Peter C, Zhao, Chanfeng, Hachmann, John P, Shevchenko, Veronika, Srinivasan, Anu, Barker, David L, Lebl, Michal

“…We have developed a high throughput assay for the measurement of protease activity in solution. This technology will accelerate research in functional…”
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Immunospecific Reduction of Antioligonucleotide Antibody-Forming Cells with a Tetrakis-oligonucleotide Conjugate (LJP 394), a Therapeutic Candidate for the Treatment of Lupus Nephritis by Jones, David S, Barstad, Paul A, Feild, Mark J, Hachmann, John P, Hayag, Merle S, Hill, Kenneth W, Iverson, G. Michael, Livingston, Douglas A, Palanki, Moorthy S

“…A discrete tetravalent conjugate, 7a (LJP 394), consisting of four oligonucleotides attached to a common carrier or platform was prepared. Single-stranded…”
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Conjugates of Double-Stranded Oligonucleotides with Poly(ethylene glycol) and Keyhole Limpet Hemocyanin: A Model for Treating Systemic Lupus Erythematosus by Jones, David S, Hachmann, John P, Osgood, Stephen A, Hayag, Merle S, Barstad, Paul A, Iverson, G. Michael, Coutts, Stephen M

“…Two types of oligonucleotides were synthesized with linker groups attached at the 5'-end. Both were repeating dimers of deoxyribocytidine and…”
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THE REACTIONS OF GUANOSINE AND OTHER NUCLEIC ACID DERIVATIVES WITH GLYOXAL, NINHYDRIN, AND RELATED COMPOUNDS. THE PREPARATION OF A NOVEL BIACETYL SELF CONDENSATION PRODUCT by HACHMANN, JOHN P

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