Search Results - "Hachmann, John"

Models of protein modification in Tris–glycine and neutral pH Bis–Tris gels during electrophoresis: Effect of gel pH by Hachmann, John P., Amshey, Joseph W.

“…The pH of conventional Tris–glycine SDS–PAGE gels during a run is determined to be 9.5, in contrast to Bis–Tris–Mes gels where the pH is 7.2. Concentrations of…”
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A high-complexity, multiplexed solution-phase assay for profiling protease activity on microarrays by Kozlov, Igor A, Melnyk, Peter C, Hachmann, John P, Srinivasan, Anu, Shults, Melissa, Zhao, Chanfeng, Musmacker, Joseph, Nelson, Nicholas, Barker, David L, Lebl, Michal

“…We have developed a miniaturized and multiplexed solution assay for the measurement of protease activity in complex samples. This technology can accelerate…”
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A Multiplexed Protein Kinase Assay by Shults, Melissa D., Kozlov, Igor A., Nelson, Nicholas, Kermani, Bahram G., Melnyk, Peter C., Shevchenko, Veronika, Srinivasan, Anu, Musmacker, Joseph, Hachmann, John P., Barker, David L., Lebl, Michal, Zhao, Chanfeng

“…We report a novel protein kinase assay designed for high‐throughput detection of one or many kinases in a complex mixture. A solution‐phase phosphorylation…”
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A method for rapid protease substrate evaluation and optimization by Kozlov, Igor A, Melnyk, Peter C, Zhao, Chanfeng, Hachmann, John P, Shevchenko, Veronika, Srinivasan, Anu, Barker, David L, Lebl, Michal

“…We have developed a high throughput assay for the measurement of protease activity in solution. This technology will accelerate research in functional…”
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Synthesis of Poly d(G-C) Oligonucleotides by Hachmann, John P., Lebl, Michal

“…Model sequences for evaluation of the GC dimer sequence repetition on synthesis success were prepared and analyzed by HPLC. Contiguous d(G-C) or d(C-G)…”
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Evaluation of Different Chemical Strategies for Conjugation of Oligonucleotides to Peptides by Kozlov, Igor A., Melnyk, Peter C., Hachmann, John P., Barker, David L., Lebl, Michal, Zhao, Chanfeng

“…We developed novel assays for high-throughput detection of one or many kinases or proteases. The assays use hundreds of different peptide substrates, each…”
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Retention of Histidine-Containing Peptides on a Nickel Affinity Column by Kozlov, Igor A., Kermani, Bahram G., Melnyk, Peter C., Barker, David L., Zhao, Chanfeng, Hachmann, John P., Lebl, Michal

“…The retention of histidine-containing peptides in immobilized metal-affinity chromatography is studied using several hundred modeled peptides. Retention is…”
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Immunospecific Reduction of Antioligonucleotide Antibody-Forming Cells with a Tetrakis-oligonucleotide Conjugate (LJP 394), a Therapeutic Candidate for the Treatment of Lupus Nephritis by Jones, David S, Barstad, Paul A, Feild, Mark J, Hachmann, John P, Hayag, Merle S, Hill, Kenneth W, Iverson, G. Michael, Livingston, Douglas A, Palanki, Moorthy S

“…A discrete tetravalent conjugate, 7a (LJP 394), consisting of four oligonucleotides attached to a common carrier or platform was prepared. Single-stranded…”
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Alternative to Piperidine in Fmoc Solid-Phase Synthesis by Hachmann, John, Lebl, Michal

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Conjugates of Double-Stranded Oligonucleotides with Poly(ethylene glycol) and Keyhole Limpet Hemocyanin: A Model for Treating Systemic Lupus Erythematosus by Jones, David S, Hachmann, John P, Osgood, Stephen A, Hayag, Merle S, Barstad, Paul A, Iverson, G. Michael, Coutts, Stephen M

“…Two types of oligonucleotides were synthesized with linker groups attached at the 5'-end. Both were repeating dimers of deoxyribocytidine and…”
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High-throughput peptide synthesis by Lebl, Michal, Hachmann, John

“…The methodologies of high-throughput peptide synthesis are overviewed and discussed. Particular focus is given to the techniques applicable to laboratories…”
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Search for optimal coupling reagent in multiple peptide synthesizer by Hachmann, John, Lebl, Michal

“…Ten different coupling reagents and their combinations were tested in parallel in the synthesis of four model peptide sequences. Significant differences were…”
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The Reaction of Guanine Derivatives with 1,2-Dicarbonyl Compounds by Shapiro, Robert, Hachmann, John

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Economical Parallel Oligonucleotide and Peptide Synthesizer – Pet Oligator by Lebl, Michal, Pistek, Christine, Hachmann, John, Mudra, Petr, Pesek, Vaclav, Pokorny, Vit, Poncar, Pavel, Zenisek, Karel

“…We have developed a small benchtop oligonucleotide synthesizer which allows the scientist to prepare, rapidly and economically, up to 24 oligonucleotides in…”
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THE REACTIONS OF GUANOSINE AND OTHER NUCLEIC ACID DERIVATIVES WITH GLYOXAL, NINHYDRIN, AND RELATED COMPOUNDS. THE PREPARATION OF A NOVEL BIACETYL SELF CONDENSATION PRODUCT by HACHMANN, JOHN P

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A New Self-Condensation Product of Biacetyl 1a by Shapiro, Robert, Hachmann, John, Wahl, Renee

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A New Self-Condensation Product of Biacetyl1a by Shapiro, Robert, Hachmann, John, Wahl, Renee

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Studies on the role of factor Ts in polypeptide synthesis by Weissbach, H, Miller, D L, Hachmann, J

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Purification of factor Ts: studies on the formation and stability of nucleotide complexes containing transfer factor Tu by Hachmann, J, Miller, D L, Weissbach, H

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Studies on the role of factor Ts in aminoacyl-tRNA binding to ribosomes by Weissbach, H, Redfield, B, Hachmann, J

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