A new method for rapid screening of ester-producing yeasts using in situ HS-SPME

The selection of ester-producing yeasts is difficult because these molecules evaporate quickly, are extremely unstable and may be missed during analytical manipulation. We propose an easy, fast and efficient headspace-SPME method for screening of ester-producing yeasts directly at the extraction via...

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Bibliographic Details
Published in:	Journal of microbiological methods Vol. 103; pp. 1 - 2
Main Authors:	Garavaglia, Juliano, Habekost, Andressa, Bjerk, Thiago Rodrigues, de Souza Schneider, Rosana de Cassia, Welke, Juliane Elisa, Zini, Cláudia Alcaraz, Valente, Patricia
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01-08-2014
Subjects:	Esters Esters - chemistry Esters - metabolism Gas chromatography Gas Chromatography-Mass Spectrometry Mass spectrometer Screening Solid Phase Microextraction - methods Yeast fermentation Yeasts - metabolism Screening Yeast fermentation Gas chromatography Esters Mass spectrometer
Online Access:	Get full text
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Summary:	The selection of ester-producing yeasts is difficult because these molecules evaporate quickly, are extremely unstable and may be missed during analytical manipulation. We propose an easy, fast and efficient headspace-SPME method for screening of ester-producing yeasts directly at the extraction vials (in situ HS-SPME). •The solid phase microextraction (SPME) is recommended for ester extraction.•HS-SPME-CG-MS analysis is an easy way to select yeast producers of esters.•The method has a very good sensitivity and reproducibility.•A good linearity at a wide range of concentrations of 0.05 to 100mg/L was reached.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0167-7012 1872-8359
DOI:	10.1016/j.mimet.2014.05.001