Search Results - "Haase, Astrid D"
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An introduction to PIWI-interacting RNAs (piRNAs) in the context of metazoan small RNA silencing pathways
Published in RNA biology (31-12-2022)“…PIWI proteins and their associated PIWI-interacting RNAs (piRNAs) constitute a small RNA-based adaptive immune system that restricts the deleterious activity…”
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The Structure of Human Argonaute-2 in Complex with miR-20a
Published in Cell (06-07-2012)“…Argonaute proteins lie at the heart of the RNA-induced silencing complex (RISC), wherein they use small RNA guides to recognize targets. Initial insight into…”
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3
A non-radioactive, improved PAR-CLIP and small RNA cDNA library preparation protocol
Published in Nucleic acids research (07-05-2021)“…Abstract Crosslinking and immunoprecipitation (CLIP) methods are powerful techniques to interrogate direct protein-RNA interactions and dissect…”
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Themes and variations on piRNA-guided transposon control
Published in Mobile DNA (02-09-2023)“…PIWI-interacting RNAs (piRNAs) are responsible for preventing the movement of transposable elements in germ cells and protect the integrity of germline…”
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The Making of a Slicer: Activation of Human Argonaute-1
Published in Cell reports (Cambridge) (01-06-2013)“…Argonautes are the central protein component in small RNA silencing pathways. Of the four human Argonautes (hAgo1–hAgo4) only hAgo2 is an active slicer. We…”
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Decoding the 5′ nucleotide bias of PIWI-interacting RNAs
Published in Nature communications (19-02-2019)“…PIWI-interacting RNAs (piRNAs) are at the center of a small RNA-based immune system that defends genomes against the deleterious action of mobile genetic…”
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Functional editing of endogenous genes through rapid selection of cell pools (Rapid generation of endogenously tagged genes in Drosophila ovarian somatic sheath cells)
Published in Nucleic acids research (26-08-2022)“…Abstract The combination of genome-editing and epitope tagging provides a powerful strategy to study proteins with high affinity and specificity while…”
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Dephosphorylation of Tyrosine 393 in Argonaute 2 by Protein Tyrosine Phosphatase 1B Regulates Gene Silencing in Oncogenic RAS-Induced Senescence
Published in Molecular cell (04-09-2014)“…Oncogenic RAS (H-RASV12) induces premature senescence in primary cells by triggering production of reactive oxygen species (ROS), but the molecular role of ROS…”
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A comparative roadmap of PIWI-interacting RNAs across seven species reveals insights into de novo piRNA-precursor formation in mammals
Published in Cell reports (Cambridge) (22-10-2024)“…PIWI-interacting RNAs (piRNAs) play a crucial role in safeguarding genome integrity by silencing mobile genetic elements. From flies to humans, piRNAs…”
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Casposase structure and the mechanistic link between DNA transposition and spacer acquisition by CRISPR-Cas
Published in eLife (08-01-2020)“…Key to CRISPR-Cas adaptive immunity is maintaining an ongoing record of invading nucleic acids, a process carried out by the Cas1-Cas2 complex that integrates…”
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Hierarchical length and sequence preferences establish a single major piRNA 3′-end
Published in iScience (17-06-2022)“…PIWI-interacting RNAs (piRNAs) guard germline genomes against the deleterious action of mobile genetic elements. PiRNAs use extensive base-pairing to recognize…”
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A Small RNA-Based Immune System Defends Germ Cells against Mobile Genetic Elements
Published in Stem cells international (01-01-2016)“…Transposons are mobile genetic elements that threaten the survival of species by destabilizing the germline genomes. Limiting the spread of these selfish…”
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Aberrant expression of select piRNA-pathway genes does not reactivate piRNA silencing in cancer cells
Published in Proceedings of the National Academy of Sciences - PNAS (04-06-2019)“…Germline genes that are aberrantly expressed in nongermline cancer cells have the potential to be ideal targets for diagnosis and therapy due to their…”
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The RNA-binding protein KSRP promotes the biogenesis of a subset of microRNAs
Published in Nature (London) (18-06-2009)“…Regulating the microRNA regulators As small RNAs exert profound effects on development and regulation of gene expression, their own expression must be…”
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The structural biochemistry of Zucchini implicates it as a nuclease in piRNA biogenesis
Published in Nature (London) (08-11-2012)“…piRNAs act to protect the genome from the damaging effect of unrestrained expression of mobile elements; here it is suggested that the phosphodiesterase…”
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Argonaute-miRNA Complexes Silence Target mRNAs in the Nucleus of Mammalian Stem Cells
Published in Molecular cell (20-09-2018)“…In mammals, gene silencing by the RNA-induced silencing complex (RISC) is a well-understood cytoplasmic posttranscriptional gene regulatory mechanism. Here, we…”
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PiRNAs Rise to Rescue Koalas
Published in Cell (17-10-2019)“…PIWI-interacting small RNAs (piRNAs) establish sequence-specific adaptive restriction of resident genomic parasites to guard genome integrity. In this issue of…”
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PIWI-interacting RNAs: who, what, when, where, why, and how
Published in The EMBO journal (26-09-2024)“…PIWI-interacting RNAs (piRNA) suppress selfish genetic elements and are essential for germ cell biology in animals. They also play critical roles in…”
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Cellular abundance shapes function in piRNA-guided genome defense
Published in Genome research (01-11-2021)“…Defense against genome invaders universally relies on RNA-guided immunity. Prokaryotic CRISPR-Cas and eukaryotic RNA interference pathways recognize targets by…”
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Nuclear PKM2 binds pre-mRNA at folded G-quadruplexes and reveals their gene regulatory role
Published in Molecular cell (03-10-2024)“…Nuclear localization of the metabolic enzyme PKM2 is widely observed in various cancer types. We identify nuclear PKM2 as a non-canonical RNA-binding protein…”
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