Search Results - "HUSE, William D"

Ultra-potent Antibodies Against Respiratory Syncytial Virus: Effects of Binding Kinetics and Binding Valence on Viral Neutralization by Wu, Herren, Pfarr, David S., Tang, Ying, An, Ling-Ling, Patel, Nita K., Watkins, Jeffry D., Huse, William D., Kiener, Peter A., Young, James F.

“…We describe here the selection of ultra-potent anti-respiratory syncytial virus (RSV) antibodies for preventing RSV infection. A large number of antibody…”
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Peptide Binding and Presentation by Mouse CD1 by Castaño, A. Raúl, Tangri, Shabnam, Jeffrey E. W. Miller, Holcombe, Hilda R., Jackson, Michael R., Huse, William D., Kronenberg, Mitchell, Peterson, Per A.

“…CD1 molecules are distantly related to the major histocompatibility complex (MHC) class I proteins. They are of unknown function. Screening random peptide…”
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Generation of a Large Combinatorial Library of the Immunoglobulin Repertoire in Phage Lambda by Huse, William D., Sastry, Lakshmi, Iverson, Sheila A., Kang, Angray S., Alting-Mees, Michelle, Burton, Dennis R., Benkovic, Stephen J., Lerner, Richard A.

“…A novel bacteriophage lambda vector system was used to express in Escherichia coli a combinatorial library of Fab fragments of the mouse antibody repertoire…”
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Stepwise in vitro Affinity Maturation of Vitaxin, an αvβ3-specific Humanized mAb by Wu, Herren, Beuerlein, Gregory, Nie, Ying, Smith, Heidi, Lee, Bruce A., Hensler, Mary, Huse, William D., Watkins, Jeffry D.

“…A protein engineering strategy based on efficient and focused mutagenesis implemented by codon-based mutagenesis was developed. Vitaxin, a humanized version of…”
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Optimization of protein therapeutics by directed evolution by Vasserot, Alain P, Dickinson, Craig D, Tang, Ying, Huse, William D, Manchester, Keith S, Watkins, Jeffry D

“…Directed evolution is a broadly applicable technology platform that is ideally suited to address the need for protein optimization and to fully exploit the…”
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Cloning, isolation and characterization of human tumor in situ monoclonal antibodies by WU, Herren, PANCOOK, James D, BEUERLEIN, Greg, CHILTON, Todd, PECHT, Gerlinde, HUSE, William D, WATKINS, Jeffry D

“…A bacterially expressed human antibody (Ab) library (diversity approximately 10(5)) was generated from tumor-infiltrating B lymphocytes present in tissue…”
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A Combinatorial Library Strategy for the Rapid Humanization of Anticarcinoma BR96 Fab by Rosok, M J, Yelton, D E, Harris, L J, Bajorath, J, Hellström, K E, Hellström, I, Cruz, G A, Kristensson, K, Lin, H, Huse, W D, Glaser, S M

“…We have used a combinatorial mutagenesis strategy to humanize BR96, a monoclonal antibody that binds to the Lewis Y class of tumor antigens. This approach…”
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Use of a Peptide Mimotope to Guide the Humanization of MRK-16, an Anti-P-glycoprotein Monoclonal Antibody by Tang, Ying, Beuerlein, Greg, Pecht, Gerlinde, Chilton, Todd, Huse, William D., Watkins, Jeffry D.

“…A mimotope-guided strategy for engineering antibodies directed against orphan targets or antigens that are difficult to purify was developed and used to…”
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λ ZAP: a bacteriophage λ expression vector with in vivo excision properties by SHORT, J. M, FERNANDEZ, J. M, SORGE, J. A, HUSE, W. D

“…A lambda insertion type cDNA cloning vector, Lambda ZAP, has been constructed. In E. coli a phagemid, pBluescript SK(-), contained within the vector, can be…”
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In vitro affinity maturation of human IgM antibodies reactive with tumor-associated antigens by Pancook, J D, Beuerlein, G, Pecht, G, Tang, Y, Nie, Y, Wu, H, Huse, W D, Watkins, J D

“…Human lymphocytes secreting tumor cell-specific IgM antibodies were enriched in vitro following the stimulation of allogeneic human splenocytes from…”
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Humanization of a murine monoclonal antibody by simultaneous optimization of framework and CDR residues by Wu, H, Nie, Y, Huse, W D, Watkins, J D

“…Optimal protein function often depends on co-operative interactions between amino acid residues distant in the protein primary sequence yet spatially near one…”
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Humanization of a murine monoclonal antibody by simultaneous optimization of framework and CDR residues 1 1Edited by J. A. Wells by Wu, Herren, Nie, Ying, Huse, William D, Watkins, Jeffry D

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Antibody engineering by codon-based mutagenesis in a filamentous phage vector system by Glaser, SM, Yelton, DE, Huse, WD

“…A novel codon-based mutagenesis procedure is described that allows rapid and efficient modification of antibody amino acid sequences expressed as F(ab)…”
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Targeted Antiangiogenic Therapy for Cancer Using Vitaxin: A Humanized Monoclonal Antibody to the Integrin ανβ3 by John C. Gutheil, Thomas N. Campbell, Paul R. Pierce, Jeffry D. Watkins, William D. Huse, David J. Bodkin, David A. Cheresh

“…Angiogenesis plays a central role in the growth and metastasis of cancers. Strategies aimed at interfering with tumor blood supply offer promise for new cancer…”
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Determination of the Relative Affinities of Antibody Fragments Expressed inEscherichia coliby Enzyme-Linked Immunosorbent Assay by Watkins, Jeffry D., Beuerlein, Gregory, Pecht, Gerlinde, McFadden, Paul R., Glaser, Scott M., Huse, William D.

“…We have previously utilized an M13 phage expression system and codon-based mutagenesis to increase the avidity of the tumor-specific antibody, BR96, 65-fold…”
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Stepwise in vitro affinity maturation of Vitaxin, an α v β 3 -specific humanized mAb by Wu, Herren, Beuerlein, Gregory, Nie, Ying, Smith, Heidi, Lee, Bruce A., Hensler, Mary, Huse, William D., Watkins, Jeffry D.

“…A protein engineering strategy based on efficient and focused mutagenesis implemented by codon-based mutagenesis was developed. Vitaxin, a humanized version of…”
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Discovery of Human Antibodies to Cell Surface Antigens by Capture Lift Screening of Phage-Expressed Antibody Libraries by Watkins, Jeffry D., Beuerlein, Gregory, Wu, Herren, McFadden, Paul R., Pancook, James D., Huse, William D.

“…An assay for the rapid identification and cloning of antibody fragments (Fabs) reactive with cell surface antigens was established and used to identify Fabs…”
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A combinatorial method for constructing libraries of long peptides displayed by filamentous phage by Haaparanta, T, Huse, W D

“…We describe the construction and screening of a random peptide library displayed by filamentous phage. The peptides are expressed in multiple copies on the…”
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Determination of the relative affinities of antibody fragments expressed in Escherichia coli by enzyme-linked immunosorbent assay by Watkins, J D, Beuerlein, G, Pecht, G, McFadden, P R, Glaser, S M, Huse, W D

“…We have previously utilized an M13 phage expression system and codon-based mutagenesis to increase the avidity of the tumor-specific antibody, BR96, 65-fold…”
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Antigen-presenting function of the TL antigen and mouse CD1 molecules by Cheroutre, H, Holcombe, H R, Tangri, S, Castaño, A R, Teitell, M, Miller, J E, Cardell, S, Benoist, C, Mathis, D, Huse, W D

“…The hallmark of all the nonclassical antigen-presenting molecules, including nonclassical class I and nonclassical class II (Karlsson et al. 1992) molecules,…”
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