Search Results - "HOZAK, Pavel"

The effect of fixation-induced cell blebbing: How to minimize a loss of proteins from cells? by Hozak, Pavel

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Nuclear patterns of phosphatidylinositol 4,5‐ and 3,4‐bisphosphate revealed by super‐resolution microscopy differ between the consecutive stages of RNA polymerase II transcription by Hoboth, Peter, Sztacho, Martin, Hozák, Pavel

“…Phosphatidylinositol phosphates are powerful signaling molecules that orchestrate signaling and direct membrane trafficking in the cytosol. Interestingly,…”
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How Single-Molecule Localization Microscopy Expanded Our Mechanistic Understanding of RNA Polymerase II Transcription by Hoboth, Peter, Šebesta, Ondřej, Hozák, Pavel

“…Classical models of gene expression were built using genetics and biochemistry. Although these approaches are powerful, they have very limited consideration of…”
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Nanoscale mapping of nuclear phosphatidylinositol phosphate landscape by dual-color dSTORM by Hoboth, Peter, Sztacho, Martin, Šebesta, Ondřej, Schätz, Martin, Castano, Enrique, Hozák, Pavel

“…Current models of gene expression, which are based on single-molecule localization microscopy, acknowledge protein clustering and the formation of…”
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PIP2 epigenetically represses rRNA genes transcription interacting with PHF8 by Ulicna, Livia, Kalendova, Alzbeta, Kalasova, Ilona, Vacik, Tomas, Hozák, Pavel

“…Phosphoinositides are present in the plasma membrane, cytoplasm and inside the cell nucleus. Here we identify phosphatidylinositol-4,5-bisphosphate (PIP2) as a…”
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Mitochondrial Genome Acquisition Restores Respiratory Function and Tumorigenic Potential of Cancer Cells without Mitochondrial DNA by Tan, An S., Baty, James W., Dong, Lan-Feng, Bezawork-Geleta, Ayenachew, Endaya, Berwini, Goodwin, Jacob, Bajzikova, Martina, Kovarova, Jaromira, Peterka, Martin, Yan, Bing, Pesdar, Elham Alizadeh, Sobol, Margarita, Filimonenko, Anatolyj, Stuart, Shani, Vondrusova, Magdalena, Kluckova, Katarina, Sachaphibulkij, Karishma, Rohlena, Jakub, Hozak, Pavel, Truksa, Jaroslav, Eccles, David, Haupt, Larisa M., Griffiths, Lyn R., Neuzil, Jiri, Berridge, Michael V.

“…We report that tumor cells without mitochondrial DNA (mtDNA) show delayed tumor growth, and that tumor formation is associated with acquisition of mtDNA from…”
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The F-Actin-Binding MPRIP Forms Phase-Separated Condensates and Associates with PI(4,5)P2 and Active RNA Polymerase II in the Cell Nucleus by Balaban, Can, Sztacho, Martin, Blažíková, Michaela, Hozák, Pavel

“…Here, we provide evidence for the presence of Myosin phosphatase rho-interacting protein (MPRIP), an F-actin-binding protein, in the cell nucleus. The MPRIP…”
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Phospholipids and inositol phosphates linked to the epigenome by Uličná, Lívia, Paprčková, Darina, Fáberová, Veronika, Hozák, Pavel

“…Even though the majority of knowledge about phospholipids comes from their cytoplasmic functions, in the last decade, it has been shown that nuclear…”
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Lamin A/C and PI(4,5)P2-A Novel Complex in the Cell Nucleus by Escudeiro-Lopes, Sara, Filimonenko, Vlada V, Jarolimová, Lenka, Hozák, Pavel

“…Lamins, the nuclear intermediate filaments, are important regulators of nuclear structural integrity as well as nuclear functional processes such as DNA…”
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Colocalization coefficients evaluating the distribution of molecular targets in microscopy methods based on pointed patterns by Pastorek, Lukáš, Sobol, Margarita, Hozák, Pavel

“…In biomedical studies, the colocalization is commonly understood as the overlap between distinctive labelings in images. This term is usually associated…”
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PIP2-Effector Protein MPRIP Regulates RNA Polymerase II Condensation and Transcription by Balaban, Can, Sztacho, Martin, Antiga, Ludovica, Miladinović, Ana, Harata, Masahiko, Hozák, Pavel

“…The specific post-translational modifications of the C-terminal domain (CTD) of the Rpb1 subunit of RNA polymerase II (RNAPII) correlate with different stages…”
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Quantitative super-resolution microscopy reveals the differences in the nanoscale distribution of nuclear phosphatidylinositol 4,5-bisphosphate in human healthy skin and skin warts by Hoboth, Peter, Sztacho, Martin, Quaas, Alexander, Akgül, Baki, Hozák, Pavel

“…Imaging of human clinical formalin-fixed paraffin-embedded (FFPE) tissue sections provides insights into healthy and diseased states and therefore represents a…”
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Nuclear pore protein TPR associates with lamin B1 and affects nuclear lamina organization and nuclear pore distribution by Fišerová, Jindřiška, Maninová, Miloslava, Sieger, Tomáš, Uhlířová, Jana, Šebestová, Lenka, Efenberková, Michaela, Čapek, Martin, Fišer, Karel, Hozák, Pavel

“…The organization of the nuclear periphery is crucial for many nuclear functions. Nuclear lamins form dense network at the nuclear periphery and play a…”
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Holography microscopy as an artifact-free alternative to phase-contrast by Pastorek, Lukáš, Venit, Tomáš, Hozák, Pavel

“…Artifact-free microscopic images represent a key requirement of multi-parametric image analysis in modern biomedical research. Holography microscopy (HM) is…”
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Super-Resolution Localisation of Nuclear PI(4)P and Identification of Its Interacting Proteome by Fáberová, Veronika, Kalasová, Ilona, Krausová, Alžběta, Hozák, Pavel

“…Phosphoinositides are glycerol-based phospholipids, and they play essential roles in cellular signalling, membrane and cytoskeletal dynamics, cell movement,…”
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Dual-color dSTORM imaging and ThunderSTORM image reconstruction and analysis to study the spatial organization of the nuclear phosphatidylinositol phosphates by Hoboth, Peter, Šebesta, Ondřej, Sztacho, Martin, Castano, Enrique, Hozák, Pavel

“…Single molecule localization microscopy (SMLM) provided an unprecedented insight into the sub-nuclear organization of proteins and nucleic acids but apart from…”
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Involvement of phosphatidylinositol 4,5-bisphosphate in RNA polymerase I transcription by Yildirim, Sukriye, Castano, Enrique, Sobol, Margarita, Philimonenko, Vlada V, Dzijak, Rastislav, Venit, Tomás, Hozák, Pavel

“…RNA polymerase I (Pol I) transcription is essential for the cell cycle, growth and protein synthesis in eukaryotes. In the present study, we found that…”
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Limited Proteolysis-Coupled Mass Spectrometry Identifies Phosphatidylinositol 4,5-Bisphosphate Effectors in Human Nuclear Proteome by Sztacho, Martin, Šalovská, Barbora, Červenka, Jakub, Balaban, Can, Hoboth, Peter, Hozák, Pavel

“…Specific nuclear sub-compartments that are regions of fundamental processes such as gene expression or DNA repair, contain phosphoinositides (PIPs). PIPs thus…”
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γ-Tubulin interacts with E2F transcription factors to regulate proliferation and endocycling in Arabidopsis by Kállai, Brigitta M, Kourová, Hana, Chumová, Jana, Papdi, Csaba, Trögelová, Lucie, Kofroňová, Olga, Hozák, Pavel, Filimonenko, Vlada, Mészáros, Tamas, Magyar, Zoltan, Bögre, Laszlo, Binarová, Pavla

“…Abstract γ-Tubulin is associated with microtubule nucleation, but evidence is accumulating in eukaryotes that it also functions in nuclear processes and in…”
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Multiple Aspects of PIP2 Involvement in C. elegans Gametogenesis by Ulicna, Livia, Rohozkova, Jana, Hozak, Pavel

“…One of the most studied phosphoinositides is phosphatidylinositol 4,5-bisphosphate (PIP2), which localizes to the plasma membrane, nuclear speckles, small foci…”
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