Expression of the Serpin Centerin Defines a Germinal Center Phenotype in B-Cell Lymphomas

Centerin (SERPINA9/GCET1) is a protease inhibitor with expression restricted to germinal center B cells and lymphoid malignancies with germinal center B-cell maturation. Expression of the centerin gene transcript, along with bcl-6 and GCET2/HGAL, constitutes a molecular signature associated with a g...

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Bibliographic Details
Published in:	American journal of clinical pathology Vol. 130; no. 1; pp. 117 - 126
Main Authors:	PATERSON, Melinda A, HOSKING, Patrick S, COUGHLIN, Paul B
Format:	Journal Article
Language:	English
Published:	Chicago, IL American Society of Clinical Pathologists 01-07-2008
Subjects:	Antibodies, Monoclonal Biological and medical sciences Cell Line, Tumor Germinal Center - metabolism Hematologic and hematopoietic diseases Humans Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis Lymphoma, B-Cell - metabolism Lymphoma, B-Cell - pathology Lymphoma, Follicular - metabolism Lymphoma, Follicular - pathology Lymphoma, Large B-Cell, Diffuse - metabolism Lymphoma, Large B-Cell, Diffuse - pathology Medical sciences Microscopy, Confocal Microscopy, Fluorescence Neoplasm Proteins Phenotype Serpins - biosynthesis Serpins - immunology Human Germinative center Serpin A9 Serpins B cell neoplasm Malignant hemopathy B-Lymphocyte Diffuse large B-cell lymphoma Centerin Protease Lymphoproliferative syndrome Follicular lymphoma Diffuse large B cell lymphoma Cancer Cell origin
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Summary:	Centerin (SERPINA9/GCET1) is a protease inhibitor with expression restricted to germinal center B cells and lymphoid malignancies with germinal center B-cell maturation. Expression of the centerin gene transcript, along with bcl-6 and GCET2/HGAL, constitutes a molecular signature associated with a good prognosis in diffuse large B-cell lymphomas. A monoclonal antibody to centerin was generated and used for Western blotting, immunohistochemistry, and immunofluorescence. Centerin expression was demonstrated in Burkitt lymphoma Raji cells. An immunohistochemical survey of normal tissues showed centerin expression in germinal center B cells in lymphoid follicles in tonsil, lymph node, and lymphoid tissue in the gastrointestinal tract. Centerin was strongly expressed in most follicular lymphomas. In addition, 14 (47%) of 30 diffuse large B-cell lymphomas were positive for centerin, which correlated most closely with CD10 expression. Immunohistochemical expression of centerin further defines the germinal center cell origin of a subgroup of lymphomas.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0002-9173 1943-7722
DOI:	10.1309/9QKE68QU7B825A3U