Identification, cloning and characterisation of a new gene required for full pyruvate decarboxylase activity in Saccharomyces cerevisiae

Identification, cloning and characterisation of a new gene required for full pyruvate decarboxylase activity in Saccharomyces cerevisiae

Biochemical evidence that pyruvate decarboxylase in S. cerevisiae might be constituted from two independently encoded subunits led us to question genetic evidence for a single structural gene. The main evidence for this was that three "structural" mutations appeared to be alleles of the sa...

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Bibliographic Details
Published in:Current genetics Vol. 15; no. 3; pp. 171 - 175
Main Authors: WRIGHT, A. P, HONG-LAN PNG, HARTLEY, B. S
Format: Journal Article
Language:English
Published: Heidelberg Springer 01-03-1989
Berlin
Subjects:
Alleles
Biological and medical sciences
Blotting, Northern
Carboxy-Lyases - genetics
Chromosome Mapping
Cloning, Molecular
Fundamental and applied biological sciences. Psychology
Genes, Fungal
Genes. Genome
Genetic Linkage
Molecular and cellular biology
Molecular genetics
Mutation
Plasmids
Pyruvate Decarboxylase - genetics
Saccharomyces cerevisiae - genetics
Transcription, Genetic
Yeast
Enzyme
Subunit
Fungi
Characterization
Northern blotting
Proteins
Enzymatic activity
Gene
Ascomycetes
Mutation
Molecular cloning
Southern blotting
Saccharomyces cerevisiae
Nucleic acid
Thallophyta
Pyruvate decarboxylase
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Summary:Biochemical evidence that pyruvate decarboxylase in S. cerevisiae might be constituted from two independently encoded subunits led us to question genetic evidence for a single structural gene. The main evidence for this was that three "structural" mutations appeared to be alleles of the same gene, PDC1 (Schmitt and Zimmermann 1982). We report that one of these mutations (pdc1-30) is not allelic either to other pdc1 alleles or to pdc2 mutations and therefore is has been renamed pdc3-30 thus identifying a new gene, PDC3. We have cloned the PDC3 gene, it represents a unique sequence in the genome and targeted integration shows tight linkage to the PDC3 locus. However, the size, abundance and regulation of the PDC3 transcript suggest that it does not encode a second structural gene. Possible functions for the PDC3 gene product are discussed.
Bibliography:ObjectType-Article-1
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ISSN:0172-8083
1432-0983
DOI:10.1007/BF00435502