Search Results - "HITCHMAN, Richard B"

Quantitative real-time PCR for rapid and accurate titration of recombinant baculovirus particles by Hitchman, Richard B., Siaterli, Evangelia A., Nixon, Clare P., King, Linda A.

“…We describe the use of quantitative PCR (QPCR) to titer recombinant baculoviruses. Custom primers and probe were designed to gp64 and used to calculate a…”
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Genetic modification of a baculovirus vector for increased expression in insect cells by Hitchman, Richard B, Possee, Robert D, Crombie, Andrew T, Chambers, Adam, Ho, Kim, Siaterli, Evangelia, Lissina, Olga, Sternard, Heather, Novy, Robert, Loomis, Kathryn, Bird, Louise E, Owens, Raymond J, King, Linda A

“…Generating large amounts of recombinant protein in transgenic animals is often challenging and has a number of drawbacks compared to cell culture systems. The…”
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Baculovirus as vectors for human cells and applications in organ transplantation by Hitchman, Richard B, Murguía-Meca, Fernanda, Locanto, Elisabetta, Danquah, John, King, Linda A.

“…[Display omitted] ► We discuss baculoviruses as vectors for gene therapy in ischemia reperfusion injury (IRI). ► We test different models of IRI in vitro. ►…”
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Improved expression of secreted and membrane-targeted proteins in insect cells by Hitchman, Richard B., Possee, Robert D., Siaterli, Evangelia, Richards, Kevin S., Clayton, Amber J., Bird, Louise E., Owens, Raymond J., Carpentier, David C. J., King, Fiona L., Danquah, John O., Spink, Karen G., King, Linda A.

“…Secretory and membrane‐bound proteins are generally produced in lower amounts in insect cells compared with cytoplasmic and nuclear proteins. There may be many…”
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BacMam Delivery of a Protective Gene to Reduce Renal Ischemia-Reperfusion Injury by Hitchman, Elisabetta, Hitchman, Richard B, King, Linda A

“…Ischemia-reperfusion (I/R) injury remains the primary contributor to delayed graft function in kidney transplantation. The beneficial application of manganese…”
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Optimizing the baculovirus expression vector system by Hitchman, Richard B., Locanto, Elisabetta, Possee, Robert D., King, Linda A.

“…Baculoviruses have a unique bi-phasic life cycle and powerful promoters, which greatly facilitates their use for recombinant protein expression in insect…”
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High-Throughput Production of Proteins in E. coli for Structural Studies by Black, Charikleia, Barker, John J, Hitchman, Richard B, Kwong, Hok Sau, Festenstein, Sam, Acton, Thomas B

“…We have developed a standardized and efficient workflow for high-throughput (HT) protein expression in E. coli and parallel purification which can be tailored…”
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Generation of baculovirus vectors for the high-throughput production of proteins in insect cells by Possee, Robert D, Hitchman, Richard B, Richards, Kevin S, Mann, Susan G, Siaterli, Evangelia, Nixon, Clare P, Irving, Helen, Assenberg, Rene, Alderton, David, Owens, Raymond J, King, Linda A

“…The baculovirus expression system is one of the most popular methods used for the production of recombinant proteins but has several complex steps which have…”
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Baculovirus expression systems for recombinant protein production in insect cells by Hitchman, Richard B, Possee, Robert D, King, Linda A

“…Baculoviruses are lethal pathogens of insects, predominantly of the order Lepidoptera. These viruses have a bi-phasic life cycle, which greatly facilitates…”
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Baculovirus as delivery system for gene transfer during hypothermic organ preservation by Murguía-Meca, Fernanda, Plata-Muñoz, Juan J, Hitchman, Richard B, Danquah, John O, Hughes, David, Friend, Peter J, Fuggle, Susan V, King, Linda A

“…Concerns over the safety of conventional viral vectors have limited the translation of gene transfer from an exciting experimental procedure to a successful…”
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Host mediated selection of pathogen genotypes as a mechanism for the maintenance of baculovirus diversity in the field by Hitchman, Richard B., Hodgson, Dave J., King, Linda A., Hails, Rosie S., Cory, Jenny S., Possee, Robert D.

“…The genetic diversity of many DNA virus populations in nature is unknown, but for those that have been studied it has been found to be relatively high. This is…”
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High-throughput baculovirus expression in insect cells by Hitchman, Richard B, Possee, Robert D, King, Linda A

“…Historically, it has been proved difficult to adapt the traditional baculovirus expression systems to an automated platform because of the complexity of the…”
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The role of mixotrophic protists in the population dynamics of the microbial food web in a small artificial pond by Hitchman, Richard B., Jones, Harriet L. J.

“…Summary 1 The seasonal variations of protist and rotifer populations were monitored over 1 year in a small artificial pond. Grazing rates on fluorescently…”
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Baculovirus as vectors for human cells and applications in organ transplantation: Baculoviruses in Human and Veterinary Medicine by HITCHMAN, Richard B, MURGUIA-MECA, Fernanda, LOCANTO, Elisabetta, DANQUAH, John, KING, Linda A

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