Search Results - "HIGUCHI, HIDEO"

Nonlinear Elasticity and an 8-nm Working Stroke of Single Myosin Molecules in Myofilaments by Kaya, Motoshi, Higuchi, Hideo

“…Using optical trapping and fluorescence imaging techniques, we measured the step size and stiffness of single skeletal myosins interacting with actin filaments…”
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3D rotational motion of an endocytic vesicle on a complex microtubule network in a living cell by Lee, Seohyun, Higuchi, Hideo

“…The transport dynamics of endocytic vesicles in a living cell contains essential biomedical information. Although the movement mechanism of a vesicle by motor…”
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Versatile properties of dynein molecules underlying regulation in flagellar oscillation by Fujiwara, Takashi, Shingyoji, Chikako, Higuchi, Hideo

“…Dynein is a minus-end-directed motor that generates oscillatory motion in eukaryotic flagella. Cyclic beating, which is the most significant feature of a…”
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A reverse stroke characterizes the force generation of cardiac myofilaments, leading to an understanding of heart function by Hwang, Yongtae, Washio, Takumi, Hisada, Toshiaki, Higuchi, Hideo, Kaya, Motoshi

“…Changes in the molecular properties of cardiac myosin strongly affect the interactions of myosin with actin that result in cardiac contraction and relaxation…”
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Coordinated force generation of skeletal myosins in myofilaments through motor coupling by Kaya, Motoshi, Tani, Yoshiaki, Washio, Takumi, Hisada, Toshiaki, Higuchi, Hideo

“…In contrast to processive molecular motors, skeletal myosins form a large motor ensemble for contraction of muscles against high loads. Despite numerous…”
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Mechanism of contraction rhythm homeostasis for hyperthermal sarcomeric oscillations of neonatal cardiomyocytes by Shintani, Seine A., Washio, Takumi, Higuchi, Hideo

“…The heart rhythm is maintained by oscillatory changes in [Ca 2+ ]. However, it has been suggested that the rapid drop in blood pressure that occurs with a slow…”
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Visualization Method for the Cell-Level Vesicle Transport Using Optical Flow and a Diverging Colormap by Lee, Seohyun, Kim, Hyuno, Higuchi, Hideo, Ishikawa, Masatoshi

“…Elucidation of cell-level transport mediated by vesicles within a living cell provides key information regarding viral infection processes and also drug…”
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Effect of myofibril passive elastic properties on the mechanical communication between motor proteins on adjacent sarcomeres by Washio, Takumi, Shintani, Seine A., Higuchi, Hideo, Sugiura, Seiryo, Hisada, Toshiaki

“…Rapid sarcomere lengthening waves propagate along a single muscle myofibril during spontaneous oscillatory contraction (SPOC). In asynchronous insect flight…”
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Numerical method for vesicle movement analysis in a complex cytoskeleton network by Lee, Seohyun, Kim, Hyuno, Higuchi, Hideo

“…The detection of the precise movement of a vesicle during transport in a live cell provides key information for the intracellular delivery process. Here we…”
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Oscillatory movement of a dynein-microtubule complex crosslinked with DNA origami by Abdellatef, Shimaa A, Tadakuma, Hisashi, Yan, Kangmin, Fujiwara, Takashi, Fukumoto, Kodai, Kondo, Yuichi, Takazaki, Hiroko, Boudria, Rofia, Yasunaga, Takuo, Higuchi, Hideo, Hirose, Keiko

“…Bending of cilia and flagella occurs when axonemal dynein molecules on one side of the axoneme produce force and move toward the microtubule (MT) minus end…”
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In Vivo Nano-imaging of Membrane Dynamics in Metastatic Tumor Cells Using Quantum Dots by Gonda, Kohsuke, Watanabe, Tomonobu M., Ohuchi, Noriaki, Higuchi, Hideo

“…Changes in membrane morphology and membrane protein dynamics based on its fluidity are critical for cancer metastasis. However, this subject has remained…”
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Extended Dual-Focus Microscopy for Ratiometric-Based 3D Movement Tracking by Lee, Seohyun, Kim, Hyuno, Higuchi, Hideo

“…Imaging the three-dimensional movement of small organelles in living cells can provide key information for the dynamics of drug delivery and virus transmission…”
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Overlapping Hand-over-Hand Mechanism of Single Molecular Motility of Cytoplasmic Dynein by Toba, Shiori, Watanabe, Tomonobu M., Yamaguchi-Okimoto, Lisa, Toyoshima, Yoko Yano, Higuchi, Hideo

“…Structural differences between dynein and kinesin suggest a unique molecular mechanism of dynein motility. Measuring the mechanical properties of a single…”
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Chemomechanical coupling of the forward and backward steps of single kinesin molecules by Higuchi, Hideo, Nishiyama, Masayoshi, Yanagida, Toshio

“…The molecular motor kinesin travels processively along a microtubule in a stepwise manner. Here we have studied the chemomechanical coupling of the hydrolysis…”
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Dynein arms are strain-dependent direction-switching force generators by Shingyoji, Chikako, Nakano, Izumi, Inoue, Yuichi, Higuchi, Hideo

“…Dynein is a minus‐end‐directed motor that can generate (forward) force to move along the microtubule toward its minus end. In addition, axonemal dyneins were…”
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Cationic Copolymer‐Chaperoned 2D–3D Reversible Conversion of Lipid Membranes by Shimada, Naohiko, Kinoshita, Hirotaka, Umegae, Takuma, Azumai, Satomi, Kume, Nozomi, Ochiai, Takuro, Takenaka, Tomoka, Sakamoto, Wakako, Yamada, Takayoshi, Furuta, Tadaomi, Masuda, Tsukuru, Sakurai, Minoru, Higuchi, Hideo, Maruyama, Atsushi

“…Nanosheets have thicknesses on the order of nanometers and planar dimensions in the micrometer range. Nanomaterials that are capable of converting reversibly…”
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Predictive diagnosis of the risk of breast cancer recurrence after surgery by single-particle quantum dot imaging by Gonda, Kohsuke, Miyashita, Minoru, Higuchi, Hideo, Tada, Hiroshi, Watanabe, Tomonobu M., Watanabe, Mika, Ishida, Takanori, Ohuchi, Noriaki

“…In breast cancer, the prognosis of human epidermal growth factor receptor 2 (HER2)-positive patients (20–25%) has been dramatically improved by the clinical…”
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Alternate fast and slow stepping of a heterodimeric kinesin molecule by Hirose, Keiko, Kaseda, Kuniyoshi, Higuchi, Hideo

“…A conventional kinesin molecule travels continuously along a microtubule in discrete 8-nm steps. This processive movement is generally explained by models in…”
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Planar polarity of multiciliated ependymal cells involves the anterior migration of basal bodies regulated by non-muscle myosin II by Hirota, Yuki, Meunier, Alice, Huang, Shihhui, Shimozawa, Togo, Yamada, Osamu, Kida, Yasuyuki S, Inoue, Masashi, Ito, Tsubasa, Kato, Hiroko, Sakaguchi, Masanori, Sunabori, Takehiko, Nakaya, Masa-Aki, Nonaka, Shigenori, Ogura, Toshihiko, Higuchi, Hideo, Okano, Hideyuki, Spassky, Nathalie, Sawamoto, Kazunobu

“…Motile cilia generate constant fluid flow over epithelial tissue, and thereby influence diverse physiological processes. Such functions of ciliated cells…”
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Processivity of the single-headed kinesin KIF1A through biased binding to tubulin by Hirokawa, Nobutaka, Okada, Yasushi, Higuchi, Hideo

“…Conventional isoforms of the motor protein kinesin behave functionally not as 'single molecules' but as 'two molecules' paired. This dimeric structure poses a…”
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