Search Results - "HEYDUK, Tomasz"

Real-Time Observation of Backtracking by Bacterial RNA Polymerase by Lass-Napiorkowska, Agnieszka, Heyduk, Tomasz

“…RNA polymerase (RNAP) backtracking is a backward sliding of the enzyme along DNA and RNA. It plays important roles in many essential processes in bacteria and…”
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RNA polymerase motions during promoter melting by Feklistov, Andrey, Bae, Brian, Hauver, Jesse, Lass-Napiorkowska, Agnieszka, Kalesse, Markus, Glaus, Florian, Altmann, Karl-Heinz, Heyduk, Tomasz, Landick, Robert, Darst, Seth A.

“…All cellular RNA polymerases (RNAPs), from those of bacteria to those of man, possess a clamp that can open and close, and it has been assumed that the open…”
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TDP-43 Oligomerization and Phase Separation Properties Are Necessary for Autoregulation by Koehler, Lydia C, Grese, Zachary R, Bastos, Alliny C S, Mamede, Lohany D, Heyduk, Tomasz, Ayala, Yuna M

“…Loss of TDP-43 protein homeostasis and dysfunction, in particular TDP-43 aggregation, are tied to amyotrophic lateral sclerosis (ALS) and frontotemporal…”
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Crystal Structure of Prothrombin Reveals Conformational Flexibility and Mechanism of Activation by Pozzi, Nicola, Chen, Zhiwei, Gohara, David W., Niu, Weiling, Heyduk, Tomasz, Di Cera, Enrico

“…The zymogen prothrombin is composed of fragment 1 containing a Gla domain and kringle-1, fragment 2 containing kringle-2, and a protease domain containing A…”
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Homogeneous Insulin and C-Peptide Sensors for Rapid Assessment of Insulin and C-Peptide Secretion by the Islets by HEYDUK, Ewa, MOXLEY, Michael M, SALVATORI, Alison, CORBETT, John A, HEYDUK, Tomasz

“…Glucose-stimulated islet insulin or C-peptide secretion experiments are a fundamental tool for studying and assessing islet function. The goal of this work was…”
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Transcription regulation of the type II restriction-modification system AhdI by Bogdanova, Ekaterina, Djordjevic, Marko, Papapanagiotou, Ioanna, Heyduk, Tomasz, Kneale, Geoff, Severinov, Konstantin

“…The Restriction-modification system AhdI contains two convergent transcription units, one with genes encoding methyltransferase subunits M and S and another…”
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Transcription regulation of restriction-modification system Esp1396I by Bogdanova, Ekaterina, Zakharova, Marina, Streeter, Simon, Taylor, James E.N., Heyduk, Tomasz, Kneale, Geoff, Severinov, Konstantin

“…The convergently transcribed restriction (R) and methylase (M) genes of the Restriction-Modification system Esp1396I are tightly regulated by a controller (C)…”
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A Consensus Adenine at Position –11 of the Nontemplate Strand of Bacterial Promoter Is Important for Nucleation of Promoter Melting by Heyduk, Ewa, Kuznedelov, Konstantin, Severinov, Konstantin, Heyduk, Tomasz

“…Numerous studies have suggested an important role of adenine at position –11 of the nontemplate strand of bacterial promoters for sequence-specific recognition…”
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DNA template sequence control of bacterial RNA polymerase escape from the promoter by Heyduk, Ewa, Heyduk, Tomasz

“…Abstract Promoter escape involves breaking of the favourable contacts between RNA polymerase (RNAP) and the promoter to allow transition to an elongation…”
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Escherichia coli RNA Polymerase Contacts outside the –10 Promoter Element Are Not Essential for Promoter Melting by Niedziela-Majka, Anita, Heyduk, Tomasz

“…We examined the relative affinity of model promoter constructs for binding Escherichia coli RNA polymerase (RNAP) holoenzyme. Model promoter constructs were…”
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Kinetics of promoter escape by bacterial RNA polymerase: effects of promoter contacts and transcription bubble collapse by Ko, Je, Heyduk, Tomasz

“…Promoter escape by RNA polymerase, the transition between the initiation and elongation, is a critical step that defines transcription output at many…”
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Phosphorylation Site Mutations in Heterochromatin Protein 1 (HP1) Reduce or Eliminate Silencing Activity by Zhao, Tao, Heyduk, Tomasz, Eissenberg, Joel C.

“…HP1 is an essential heterochromatin-associated protein in Drosophila. HP1 has dosage-dependent effects on the silencing of euchromatic genes that are…”
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Zinc Inhibition of cAMP Signaling by Klein, Claudette, Sunahara, Roger K., Hudson, Tracie Y., Heyduk, Tomasz, Howlett, Allyn C.

“…Zn2+ is required as either a catalytic or structural component for a large number of enzymes and thus contributes to a variety of important biological…”
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Luminescence Resonance Energy Transfer-Based High-Throughput Screening Assay for Inhibitors of Essential Protein-Protein Interactions in Bacterial RNA Polymerase by BERGENDAHL, Veit, HEYDUK, Tomasz, BURGESS, Richard R

“…Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit…”
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Ribosome display enhanced by next generation sequencing: A tool to identify antibody-specific peptide ligands by Heyduk, Ewa, Heyduk, Tomasz

“…Detection of antibodies in serum has many important applications. Our goal was to develop a facile general experimental approach for identifying…”
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Next Generation Sequencing-Based Parallel Analysis of Melting Kinetics of 4096 Variants of a Bacterial Promoter by Heyduk, Ewa, Heyduk, Tomasz

“…Promoter melting by bacterial RNA polymerase is a key step in transcription initiation. We used a next generation sequencing (NGS) based approach to analyze in…”
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Bivalent Ligands with Long Nanometer-Scale Flexible Linkers by Tian, Ling, Heyduk, Tomasz

“…High-affinity ligands recognizing biomolecules with high specificity are crucial for drug discovery and biomolecule detection. We describe here a simple…”
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Next Generation Sequencing-based analysis of RNA polymerase functions by Heyduk, Tomasz, Heyduk, Ewa

“…•NGS could be used as a reliable quantitative readout in in vitro experiments.•RNAP activity on thousands of DNA template sequence variants can be studied in…”
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Measuring protein conformational changes by FRET/LRET by Heyduk, Tomasz

“…Fluorescence resonance energy transfer (FRET) provides a unique means of measuring interatomic distances in biological molecules in real time. Recent advances…”
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Fluorescent homogeneous immunosensors for detecting pathogenic bacteria by Heyduk, Ewa, Heyduk, Tomasz

“…We developed a straightforward antibody-based assay for rapid homogeneous detection of bacteria. Our sensors utilize antibody recognizing cell-surface epitopes…”
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