Search Results - "HEYDUK, Ewa"

Homogeneous Insulin and C-Peptide Sensors for Rapid Assessment of Insulin and C-Peptide Secretion by the Islets by HEYDUK, Ewa, MOXLEY, Michael M, SALVATORI, Alison, CORBETT, John A, HEYDUK, Tomasz

“…Glucose-stimulated islet insulin or C-peptide secretion experiments are a fundamental tool for studying and assessing islet function. The goal of this work was…”
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A Consensus Adenine at Position –11 of the Nontemplate Strand of Bacterial Promoter Is Important for Nucleation of Promoter Melting by Heyduk, Ewa, Kuznedelov, Konstantin, Severinov, Konstantin, Heyduk, Tomasz

“…Numerous studies have suggested an important role of adenine at position –11 of the nontemplate strand of bacterial promoters for sequence-specific recognition…”
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DNA template sequence control of bacterial RNA polymerase escape from the promoter by Heyduk, Ewa, Heyduk, Tomasz

“…Abstract Promoter escape involves breaking of the favourable contacts between RNA polymerase (RNAP) and the promoter to allow transition to an elongation…”
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Ribosome display enhanced by next generation sequencing: A tool to identify antibody-specific peptide ligands by Heyduk, Ewa, Heyduk, Tomasz

“…Detection of antibodies in serum has many important applications. Our goal was to develop a facile general experimental approach for identifying…”
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Next Generation Sequencing-Based Parallel Analysis of Melting Kinetics of 4096 Variants of a Bacterial Promoter by Heyduk, Ewa, Heyduk, Tomasz

“…Promoter melting by bacterial RNA polymerase is a key step in transcription initiation. We used a next generation sequencing (NGS) based approach to analyze in…”
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Next Generation Sequencing-based analysis of RNA polymerase functions by Heyduk, Tomasz, Heyduk, Ewa

“…•NGS could be used as a reliable quantitative readout in in vitro experiments.•RNAP activity on thousands of DNA template sequence variants can be studied in…”
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Fluorescent homogeneous immunosensors for detecting pathogenic bacteria by Heyduk, Ewa, Heyduk, Tomasz

“…We developed a straightforward antibody-based assay for rapid homogeneous detection of bacteria. Our sensors utilize antibody recognizing cell-surface epitopes…”
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Peptide ligand-based ELISA reagents for antibody detection by Heyduk, Ewa, Hickey, Rachel, Pozzi, Nicola, Heyduk, Tomasz

“…Detection of specific antibodies has numerous research, therapeutic and diagnostic applications. Short peptide ligands that bind specifically to antibodies…”
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Next Generation Sequencing-Based Parallel Analysis of Melting Kinetics of 4096 Variants of a Bacterial Promoter by Heyduk, Ewa, Heyduk, Tomasz

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Homogeneous fluorescence assay for cyclic AMP by Heyduk, Ewa, Fei, Ying, Heyduk, Tomasz

“…The objective of these studies was to develop a new homogeneous fluorescence assay for determining the concentrations of cAMP in biological samples. The assay…”
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Nucleic Acid-Based Fluorescence Sensors for Detecting Proteins by Heyduk, Ewa, Heyduk, Tomasz

“…We report here development of a rapid, homogeneous, aptamer-based fluorescence assay (“molecular beacons”) for detecting proteins. The assay involves…”
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Molecular beacons for detecting DNA binding proteins by Heyduk, Tomasz, Heyduk, Ewa

“…We report here a simple, rapid, homogeneous fluorescence assay, the molecular beacon assay, for the detection and quantification of sequence-specific…”
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Fluorescent homogenous immunosensors for detecting pathogenic bacteria by Heyduk, Ewa, Heyduk, Tomasz

“…We developed a straightforward antibody-based assay for rapid homogeneous detection of bacteria. Our sensors utilize an antibody recognizing cell-surface…”
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Molecular Pincers: Antibody-Based Homogeneous Protein Sensors by Heyduk, Ewa, Dummit, Benjamin, Chang, Yie-Hwa, Heyduk, Tomasz

“…We describe here a new homogeneous antibody-based protein sensor design (molecular pincers) that allows rapid and sensitive detection of a specific protein in…”
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Promoter spacer DNA plays an active role in integrating the functional consequences of RNA polymerase contacts with − 10 and − 35 promoter elements by Sztiller-Sikorska, Malgorzata, Heyduk, Ewa, Heyduk, Tomasz

“…Bacterial RNA polymerase (RNAP) interacts with conserved − 10 and − 35 promoter elements to recognize the promoter and to form an open complex in which DNA…”
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Detection Methodology Based on Target Molecule-Induced Sequence-Specific Binding to a Single-Stranded Oligonucleotide by Lass-Napiorkowska, Agnieszka, Heyduk, Ewa, Tian, Ling, Heyduk, Tomasz

“…We have recently developed a mix-and-read format homogeneous antigen peptide based assay for detection of the antibodies (Tian, L.; Heyduk, T. Anal. Chem…”
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Conformational Changes of DNA Induced by Binding ofChironomus High Mobility Group Protein 1a (cHMG1a) by Heyduk, Ewa, Heyduk, Tomasz, Claus, Peter, Wiśniewski, Jacek R.

“…High mobility group (HMG) proteins are thought to facilitate assembly of higher order chromatin structure through modulation of DNA conformation. In this work…”
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Heterogeneous Immunosensors that Do not Require Protein Immobilization by Heyduk, Ewa, Lass, Agnieszka, Tian, Ling, Heyduk, Tomasz

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A Basal Promoter Element Recognized by Free RNA Polymerase σ Subunit Determines Promoter Recognition by RNA Polymerase Holoenzyme by Feklistov, Andrey, Barinova, Nataliya, Sevostyanova, Anastasiya, Heyduk, Ewa, Bass, Irina, Vvedenskaya, Irina, Kuznedelov, Konstantin, Merkienė, Eglė, Stavrovskaya, Elena, Klimašauskas, Saulius, Nikiforov, Vadim, Heyduk, Tomasz, Severinov, Konstantin, Kulbachinskiy, Andrey

“…During transcription initiation by bacterial RNA polymerase, the σ subunit recognizes the −35 and −10 promoter elements; free σ, however, does not bind DNA. We…”
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Molecular pincers – new antibody-based homogenous protein sensors by Heyduk, Ewa, Dummit, Benjamin, Chang, Yie-Hwa, Heyduk, Tomasz

“…We describe here a new homogenous antibody-based protein sensor design (molecular pincers) that allows rapid and sensitive detection of a specific protein in…”
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