Search Results - "HESSELS, G. I"

The steroid catabolic pathway of the intracellular pathogen Rhodococcus equi is important for pathogenesis and a target for vaccine development by van der Geize, R, Grommen, A W F, Hessels, G I, Jacobs, A A C, Dijkhuizen, L

“…Rhodococcus equi causes fatal pyogranulomatous pneumonia in foals and immunocompromised animals and humans. Despite its importance, there is currently no…”
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novel method to generate unmarked gene deletions in the intracellular pathogen Rhodococcus equi using 5-fluorocytosine conditional lethality by van der Geize, R, de Jong, W, Hessels, G.I, Grommen, A.W.F, Jacobs, A.A.C, Dijkhuizen, L

“…A novel method to efficiently generate unmarked in-frame gene deletions in Rhodococcus equi was developed, exploiting the cytotoxic effect of 5-fluorocytosine…”
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Targeted disruption of the kstD gene encoding a 3-ketosteroid delta(1)-dehydrogenase isoenzyme of Rhodococcus erythropolis strain SQ1 by van Der Geize, R, Hessels, G I, van Gerwen, R, Vrijbloed, J W, van Der Meijden, P, Dijkhuizen, L

“…Microbial phytosterol degradation is accompanied by the formation of steroid pathway intermediates, which are potential precursors in the synthesis of…”
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(De)regulation of key enzyme steps in the shikimate pathway and phenylalanine-specific pathway of the actinomycete Amycolatopsis methanolica by Kloosterman, H, Hessels, G. I, Vrijbloed, J. W, Euverink, G. J, Dijkhuizen, L

“…Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, PO Box 14, 9750 AA Haren, The…”
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Characterization of a Second Rhodococcus erythropolis SQ1 3-Ketosteroid 9α-Hydroxylase Activity Comprising a Terminal Oxygenase Homologue, KshA2, Active with Oxygenase-Reductase Component KshB by van der Geize, R, Hessels, G.I, Nienhuis-Kuiper, M, Dijkhuizen, L

“…Previously we have characterized 3-ketosteroid 9α-hydroxylase (KSH), a key enzyme in microbial steroid degradation in Rhodococcus erythropolis strain SQ1, as a…”
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Chorismate mutase and 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase of the methylotrophic actinomycete Amycolatopsis methanolica by EUVERINK, G. J. W, HESSELS, G. I, FRANKE, C, DIJKHUIZEN, L

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Enzymes of glucose and methanol metabolism in the actinomycete Amycolatopsis methanolica by Alves, A M, Euverink, G J, Hektor, H J, Hessels, G I, van der Vlag, J, Vrijbloed, J W, Hondmann, D, Visser, J, Dijkhuizen, L

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Biosynthesis of l-Phenylalanine and l-Tyrosine in the Actinomycete Amycolatopsis methanolica by ABOU-ZEID, A, EUVERINK, G. J. W, HESSELS, G. I, JENSEN, R. A, DIJKHUIZEN, L

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Molecular and functional characterization of kshA and kshB, encoding two components of 3‐ketosteroid 9α‐hydroxylase, a class IA monooxygenase, in Rhodococcus erythropolis strain SQ1 by Geize, R. van der, Hessels, G. I., Gerwen, R. van, Meijden, P. van der, Dijkhuizen, L.

“…Summary 9α‐Hydroxylation of 4‐androstene‐3,17‐dione (AD) and 1,4‐androstadiene‐3,17‐dione (ADD) is catalysed by 3‐ketosteroid 9α‐hydroxylase (KSH), a key…”
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Molecular cloning with a pMEA300-derived shuttle vector and characterization of the Amycolatopsis methanolica prephenate dehydratase gene by Vrijbloed, J W, van Hylckama Vlieg, J, van der Put, N M, Hessels, G I, Dijkhuizen, L

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Characterization of a Second Rhodococcus erythropolis SQ1 3-Ketosteroid 9[alpha]-Hydroxylase Activity Comprising a Terminal Oxygenase Homologue, KshA2, Active with Oxygenase-Reductase Component KshB by van der Geize, R, Hessels, G I, Nienhuis-Kuiper, M, Dijkhuizen, L

“…Previously we have characterized 3-ketosteroid 9α-hydroxylase (KSH), a key enzyme in microbial steroid degradation in Rhodococcus erythropolis strain SQ1, as a…”
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Identification of the minimal replicon of plasmid pMEA300 of the methylotrophic actinomycete Amycolatopsis methanolica by Vrijbloed, J W, Jelínková, M, Hessels, G I, Dijkhuizen, L

“…The actinomycete Amycolatopsis methanolica contains a 13.3 kb plasmid (pMEA300), capable of enhancing the spontaneous mutation frequency of its host. Depending…”
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Targeted disruption of the kstD gene encoding a 3-ketosteroid Δ1-dehydrogenase isoenzyme of Rhodococcus erythropolis strain SQ1 by VAN DER GEIZE, R, HESSELS, G. I, VAN GERWEN, R, VRIJBLOED, J. W, VAN DER MEIJDEN, P, DIJKHUIZEN, L

“…Microbial phytosterol degradation is accompanied by the formation of steroid pathway intermediates, which are potential precursors in the synthesis of…”
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Purification and characterization of a dual function 3-dehydroquinate dehydratase from Amycolatopsis methanolica by Euverink, G.J.W, Hessels, G.I, Vrijbloed, J.W, Coggins, J.R, Dijkhuizen, L

“…Studies on hydroaromatic metabolism in the actinomycete Amycolatopsis methanolica revealed that the organism grows rapidly on quinate (but not on shikimate) as…”
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Characterization of a Second Rhodococcus erythropolis SQ1 3-Ketosteroid 9a-Hydroxylase Activity Comprising a Terminal Oxygenase Homologue, KshA2, Active with Oxygenase-Reductase Component KshB by VAN DER GEIZE, R, HESSELS, G. I, NIENHUIS-KUIPER, M, DIJKHUIZEN, L

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Isolation and analysis of mutants of the methylotrophic actinomycete Amycolatopsis methanolica blocked in aromatic amino acid biosynthesis by Euverink, G.J.W., Vrijbloed, J.W., Hesseis, G.I., Dijkhuizen, L.

“…Abstract Mutants of the actinomycete Amycolatopsis methanolica blocked in aromatic amino acid biosynthesis were isolated using brief ultrasonic treatments to…”
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Characterization of a second Rhodococcus erythropolis SQ1 3-ketosteroid 9alpha-hydroxylase activity comprising a terminal oxygenase homologue, KshA2, active with oxygenase-reductase component KshB by van der Geize, R, Hessels, G I, Nienhuis-Kuiper, M, Dijkhuizen, L

“…Previously we have characterized 3-ketosteroid 9alpha-hydroxylase (KSH), a key enzyme in microbial steroid degradation in Rhodococcus erythropolis strain SQ1,…”
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Molecular and functional characterization of the kstD2 gene of Rhodococcus erythropolis SQ1 encoding a second 3-ketosteroid Delta(1)-dehydrogenase isoenzyme by van der Geize, Robert, Hessels, Gerda I, Dijkhuizen, Lubbert

“…Previously, Rhodococcus erythropolis SQ1 kstD, encoding ketosteroid Delta(1)-dehydrogenase (KSTD1) was characterized. Surprisingly, a kstD gene deletion mutant…”
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Molecular and functional characterization of kshA and kshB, encoding two components of 3-ketosteroid 9alpha-hydroxylase, a class IA monooxygenase, in Rhodococcus erythropolis strain SQ1 by van der Geize, R, Hessels, G I, van Gerwen, R, van der Meijden, P, Dijkhuizen, L

“…9 alpha-Hydroxylation of 4-androstene-3,17-dione (AD) and 1,4-androstadiene-3,17-dione (ADD) is catalysed by 3-ketosteroid 9 alpha-hydroxylase (KSH), a key…”
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Molecular and functional characterization of kshA and kshB, encoding two components of 3-ketosteroid 9(alpha)-hydroxylase, a class IA monooxygenase, in Rhodocuccus erythropolis strain SQ1 by van der Geize, R, Hessels, G I, R Van Gerwen, van der Meijden, P, Dijkhuizen, L

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