Search Results - "HELMS, J. B"

Capacitation-dependent concentration of lipid rafts in the apical ridge head area of porcine sperm cells by van Gestel, R.A., Brewis, I.A., Ashton, P.R., Helms, J.B., Brouwers, J.F., Gadella, B.M.

“…Lipid architecture of the plasma membrane plays an important role in the capacitation process of the sperm cell. During this process, an increase in membrane…”
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Sphingomyelin-enriched microdomains at the Golgi complex by Gkantiragas, I, Brügger, B, Stüven, E, Kaloyanova, D, Li, X Y, Löhr, K, Lottspeich, F, Wieland, F T, Helms, J B

“…Sphingomyelin- and cholesterol-enriched microdomains can be isolated as detergent-resistant membranes from total cell extracts (total-DRM). It is generally…”
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Effects of methyl-β-cyclodextrin-mediated cholesterol depletion in porcine sperm compared to somatic cells by van Gestel, R.A., Helms, J.B., Brouwers, J.F.H.M., Gadella, B.M.

“…In this study, the use of methyl‐β‐cyclodextrin (MBCD) to support capacitation of sperm cells was studied. Sperm were incubated with MBCD or alternatively…”
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Characterization of different oligomeric forms of CRISP2 in the perinuclear theca versus the fibrous tail structures of boar spermatozoa by Zhang, M, Bromfield, E.G, Veenendaal, T, Klumperman, J, Helms, J.B, Gadella, B.M

“…Mammalian sperm carry a variety of highly condensed insoluble protein structures such as the perinuclear theca, the fibrous sheath and the outer dense fibers,…”
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Inhibition by brefeldin A of a Golgi membrane enzyme that catalyses exchange of guanine nucleotide bound to ARF by Helms, J. Bernd, Rothman, James E

“…A wide variety of membrane transformations important in intracellular transport are inhibited by the fungal metabolite brefeldin A (refs 1-4), implying that…”
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Hydrolysis of Bound GTP by ARF Protein Triggers Uncoating of Golgi-Derived COP-Coated Vesicles by Tanigawa, Gary, Orci, Lelio, Amherdt, Mylene, Ravazzola, Mariella, Helms, J. Bernd, Rothman, James E.

“…The cycle of nucleotide exchange and hydrolysis by a small GTP-binding protein, ADP-ribosylation factor (ARF), helps to provide vectoriality to vesicle…”
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Binding of coatomer to Golgi membranes requires ADP-ribosylation factor by PALMER, D. J, HELMS, J. B, BECKERS, C. J. M, ORCI, L, ROTHMAN, J. E

“…Coatomer, a complex of seven proteins, appears to be the precursor of the coat structure of non-clathrin-coated Golgi-derived vesicles. Another component of…”
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Bovine Cumulus Cells Protect Maturing Oocytes from Increased Fatty Acid Levels by Massive Intracellular Lipid Storage by AARDEMA, Hilde, LOLICATO, Francesca, VAN DE LEST, Chris H. A, BROUWERS, Jos F, VAANDRAGER, Arie B, VAN TOL, Helena T. A, ROELEN, Bernard A. J, VOS, Peter L. A. M, HELMS, J. Bernd, GADELLA, Bart M

“…Metabolic conditions characterized by elevated free fatty acid concentrations in blood and follicular fluid are often associated with impaired female…”
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A Major Transmembrane Protein of Golgi-Derived COPI-Coated Vesicles Involved in Coatomer Binding by Sohn, Kai, Orci, Lelio, Ravazzola, Mariella, Amherdt, Mylène, Bremser, Martina, Lottspeich, Friedrich, Fiedler, Klaus, Helms, J. Bernd, Wieland, Felix T.

“…Formation of non-clathrin-coated vesicles requires the recruitment of several cytosolic factors to the Golgi membrane. To identify membrane proteins involved…”
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The nitric oxide donor sodium nitroprusside inhibits mineralization in ATDC5 cells by Huitema, L F A, Vaandrager, A B, van Weeren, P R, Barneveld, A, Helms, J B, van de Lest, C H A

“…The aim of this study was to test whether the nitric oxide (NO) donor sodium nitroprusside (SNP) has an effect on mineralization in ATDC5 cells. Mineralization…”
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p24 and p23, the major transmembrane proteins of COPI-coated transport vesicles, form hetero-oligomeric complexes and cycle between the organelles of the early secretory pathway by Gommel, D, Orci, L, Emig, E.M, Hannah, M.J, Ravazzola, M, Nickel, W, Helms, J.B, Wieland, F.T, Sohn, K

“…COPI-coated vesicles that bud off the Golgi complex contain two major transmembrane proteins, p23 and p24. We have localized the protein at the Golgi complex…”
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Two Distinct Populations of ARF Bound to Golgi Membranes by Helms, J. Bernd, Palmer, David J., Rothman, James E.

“…ADP-ribosylation factor (ARF) is a small molecular weight GTP-binding protein (20 kD) and has been implicated in vesicular protein transport. The guanine…”
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Synthesis of beta-hematin by dehydrohalogenation of hemin by Bohle, D S, Helms, J B

“…beta-Hematin, the metalloporphyrin coordination polymer present in malaria pigment, is prepared in high yield by the abstraction of HCl from hemin with a…”
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Evidence for Segregation of Sphingomyelin and Cholesterol during Formation of COPI-Coated Vesicles by Brügger, Britta, Sandhoff, Roger, Wegehingel, Sabine, Gorgas, Karin, Malsam, Jörg, Helms, J. Bernd, Lehmann, Wolf-Dieter, Nickel, Walter, Wieland, Felix T.

“…In higher eukaryotes, phospholipid and cholesterol synthesis occurs mainly in the endoplasmic reticulum, whereas sphingomyelin and higher glycosphingolipids…”
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Reconstitution of steps in the constitutive secretory pathway in permeabilized cells. Secretion of glycosylated tripeptide and truncated sphingomyelin by Helms, J B, Karrenbauer, A, Wirtz, K W, Rothman, J E, Wieland, F T

“…The constitutive secretory pathway has been reconstituted in mechanically permeabilized Chinese hamster ovary cells using two secretory markers, an…”
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Recruitment to Golgi membranes of ADP-ribosylation factor 1 is mediated by the cytoplasmic domain of p23 by Gommel, Daniel U., Memon, Abdul R., Heiss, Armin, Lottspeich, Friedrich, Pfannstiel, Jens, Lechner, Johannes, Reinhard, Constanze, Helms, J.Bernd, Nickel, Walter, Wieland, Felix T.

“…Binding to Golgi membranes of ADP ribosylation factor 1 (ARF1) is the first event in the initiation of COPI coat assembly. Based on binding studies, a…”
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Ultrastructural localization of flotillin-1 to cholesterol-rich membrane microdomains, rafts, in rat brain tissue by Kokubo, Hideko, Helms, J.Bernd, Ohno-Iwashita, Yoshiko, Shimada, Yukiko, Horikoshi, Yuko, Yamaguchi, Haruyasu

“…There is much interest in research on cholesterol-rich membrane microdomains, rafts, in the field of neurobiology. However, no one has shown the ultrastructure…”
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Synthesis of phosphatidylinositol 4,5-bisphosphate in the endoplasmic reticulum of Chinese hamster ovary cells by BERND HELMS, J, VAN DE VRIES, K, WIRTZ, K. W. A

“…We have investigated the intracellular localization and synthesis of phosphatidylinositol 4,5-bisphosphate (PtdInsP2) in Chinese hamster ovary (CHO) cells by…”
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Uptake by COPI-coated vesicles of both anterograde and retrograde cargo is inhibited by GTPγS in vitro by Nickel, Walter, Malsam, Jörg, Gorgas, Karin, Ravazzola, Mariella, Jenne, Nicole, Helms, J. Bernd, Wieland, Felix T.

“…ABSTRACT On the basis of the cell surface protein CD8 we have constructed reporter molecules for both anterograde and retrograde transport from the Golgi…”
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Role of heterotrimeric GTP binding proteins in vesicular protein transport: indications for both classical and alternative G protein cycles by Helms, J.Bernd

“…Heterotrimeric G proteins are involved in hormonal signal transduction across the plasma membrane. Recent evidence suggests that they have a role in vesicular…”
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