Search Results - "HELMA, Jonas"

Nanobodies and recombinant binders in cell biology by Helma, Jonas, Cardoso, M Cristina, Muyldermans, Serge, Leonhardt, Heinrich

“…Antibodies are key reagents to investigate cellular processes. The development of recombinant antibodies and binders derived from natural protein scaffolds has…”
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Nanobodies: Chemical Functionalization Strategies and Intracellular Applications by Schumacher, Dominik, Helma, Jonas, Schneider, Anselm F. L., Leonhardt, Heinrich, Hackenberger, Christian P. R.

“…Nanobodies can be seen as next‐generation tools for the recognition and modulation of antigens that are inaccessible to conventional antibodies. Due to their…”
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Cysteine‐Selective Phosphonamidate Electrophiles for Modular Protein Bioconjugations by Kasper, Marc‐André, Glanz, Maria, Stengl, Andreas, Penkert, Martin, Klenk, Simon, Sauer, Tom, Schumacher, Dominik, Helma, Jonas, Krause, Eberhard, Cardoso, M. Cristina, Leonhardt, Heinrich, Hackenberger, Christian P. R.

“…We describe a new technique in protein synthesis that extends the existing repertoire of methods for protein modification: A chemoselective reaction that…”
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Visualization and targeted disruption of protein interactions in living cells by Herce, Henry D., Deng, Wen, Helma, Jonas, Leonhardt, Heinrich, Cardoso, M. Cristina

“…Protein–protein interactions are the basis of all processes in living cells, but most studies of these interactions rely on biochemical in vitro assays. Here…”
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Modulation of protein properties in living cells using nanobodies by Leonhardt, Heinrich, Hopfner, Karl-Peter, Rothbauer, Ulrich, Kirchhofer, Axel, Helma, Jonas, Schmidthals, Katrin, Frauer, Carina, Cui, Sheng, Karcher, Annette, Pellis, Mireille, Muyldermans, Serge, Casas-Delucchi, Corella S, Cardoso, M Cristina

“…GFP fluorescence can be modulated in mammalian cells by binding to single-chain antibodies (nanobodies), selected to make GFP brighter or dimmer; these changes…”
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Compact hydrophilic electrophiles enable highly efficacious high DAR ADCs with excellent in vivo PK profile by Ochtrop, Philipp, Jahzerah, Jahaziel, Machui, Paul, Mai, Isabelle, Schumacher, Dominik, Helma, Jonas, Kasper, Marc-André, Hackenberger, Christian P R

“…The recent success of antibody-drug conjugates (ADC), exemplified by seven new FDA-approvals within three years, has led to increased attention for antibody…”
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Ethynylphosphonamidates for the Rapid and Cysteine‐Selective Generation of Efficacious Antibody–Drug Conjugates by Kasper, Marc‐André, Stengl, Andreas, Ochtrop, Philipp, Gerlach, Marcus, Stoschek, Tina, Schumacher, Dominik, Helma, Jonas, Penkert, Martin, Krause, Eberhard, Leonhardt, Heinrich, Hackenberger, Christian P. R.

“…Requirements for novel bioconjugation reactions for the synthesis of antibody–drug conjugates (ADCs) are exceptionally high, since conjugation selectivity as…”
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Bis‐ethynylphosphonamidates as an Modular Conjugation Platform to Generate Multi‐Functional Protein‐ and Antibody‐Drug‐Conjugates by Kasper, Marc‐André, Lassak, Lukas, Vogl, Annette M., Mai, Isabelle, Helma, Jonas, Schumacher, Dominik, Hackenberger, Christian P. R.

“…Bis‐ethynylphosphonamidates allow for a simple chemoselective addition of two thiol‐containing modules in a row. We describe four such bis‐electrophiles that…”
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FLEXamers: A Double Tag for Universal Generation of Versatile Peptide-MHC Multimers by Effenberger, Manuel, Stengl, Andreas, Schober, Kilian, Gerget, Maria, Kampick, Maximilian, Müller, Thomas R, Schumacher, Dominik, Helma, Jonas, Leonhardt, Heinrich, Busch, Dirk H

“…Peptide-MHC (pMHC) multimers have become a valuable tool for immunological research, clinical immune monitoring, and immunotherapeutic applications…”
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Site‐Specific Antibody Fragment Conjugates for Reversible Staining in Fluorescence Microscopy by Schwach, Jonathan, Kolobynina, Ksenia, Brandstetter, Katharina, Gerlach, Marcus, Ochtrop, Philipp, Helma, Jonas, Hackenberger, Christian P. R., Harz, Hartmann, Cardoso, M. Cristina, Leonhardt, Heinrich, Stengl, Andreas

“…Antibody conjugates have taken a great leap forward as tools in basic and applied molecular life sciences that was enabled by the development of chemoselective…”
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N‐Hydroxysuccinimide‐Modified Ethynylphosphonamidates Enable the Synthesis of Configurationally Defined Protein Conjugates by Kasper, Marc‐André, Gerlach, Marcus, Schneider, Anselm F. L., Groneberg, Christiane, Ochtrop, Philipp, Boldt, Stefanie, Schumacher, Dominik, Helma, Jonas, Leonhardt, Heinrich, Christmann, Mathias, Hackenberger, Christian P. R.

“…Herein, the application of N‐hydroxysuccinimide‐modified phosphonamidate building blocks for the incorporation of cysteine‐selective ethynylphosphonamidates…”
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Magnetosome Expression of Functional Camelid Antibody Fragments (Nanobodies) in Magnetospirillum gryphiswaldense by POLLITHY, Anna, ROMER, Tina, LANG, Claus, MÜLLER, Frank D, HELMA, Jonas, LEONHARDT, Heinrich, ROTHBAUER, Ulrich, SCHÜLER, Dirk

“…Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit…”
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Direct and dynamic detection of HIV-1 in living cells by Helma, Jonas, Schmidthals, Katrin, Lux, Vanda, Nüske, Stefan, Scholz, Armin M, Kräusslich, Hans-Georg, Rothbauer, Ulrich, Leonhardt, Heinrich

“…In basic and applied HIV research, reliable detection of viral components is crucial to monitor progression of infection. While it is routine to detect…”
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Ethynylphosphonamidates for the Rapid and Cysteine‐Selective Generation of Efficacious Antibody–Drug Conjugates by Kasper, Marc‐André, Stengl, Andreas, Ochtrop, Philipp, Gerlach, Marcus, Stoschek, Tina, Schumacher, Dominik, Helma, Jonas, Penkert, Martin, Krause, Eberhard, Leonhardt, Heinrich, Hackenberger, Christian P. R.

“…Requirements for novel bioconjugation reactions for the synthesis of antibody–drug conjugates (ADCs) are exceptionally high, since conjugation selectivity as…”
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Cysteinselektive phosphonamidatbasierte Elektrophile für modulare Biokonjugationen by Kasper, Marc‐André, Glanz, Maria, Stengl, Andreas, Penkert, Martin, Klenk, Simon, Sauer, Tom, Schumacher, Dominik, Helma, Jonas, Krause, Eberhard, Cardoso, M. Cristina, Leonhardt, Heinrich, Hackenberger, Christian P. R.

“…Wir beschreiben eine neue Methode zur Proteinsynthese, die das bestehende Repertoire an Möglichkeiten zur Proteinmodifikation erweitert: Eine chemoselektive…”
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Neue Generation von Antikörper‐Wirkstoff‐Konjugaten by Schumacher, Dominik, Helma‐Smets, Jonas, Hackenberger, Christian, Leonhardt, Heinrich

“…Das angehende Spin‐Off Tubulis entwickelt einzigartige Technologien für die Funktionalisierung von Biomolekülen mit besonderem Potenzial für die Herstellung…”
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Current Status: Site-Specific Antibody Drug Conjugates by Schumacher, Dominik, Hackenberger, Christian P. R., Leonhardt, Heinrich, Helma, Jonas

“…Antibody drug conjugates (ADCs), a promising class of cancer biopharmaceuticals , combine the specificity of therapeutic antibodies with the pharmacological…”
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Compact hydrophilic electrophiles enable highly efficacious high DAR ADCs with excellent PK profile by Ochtrop, Philipp, Jahzerah, Jahaziel, Machui, Paul, Mai, Isabelle, Schumacher, Dominik, Helma, Jonas, Kasper, Marc-André, Hackenberger, Christian P. R

“…The recent success of antibody-drug conjugates (ADC), exemplified by seven new FDA-approvals within three years, has led to increased attention for antibody…”
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Intracellular chromobody delivery by mesoporous silica nanoparticles for antigen targeting and visualization in real time by Chiu, Hsin-Yi, Deng, Wen, Engelke, Hanna, Helma, Jonas, Leonhardt, Heinrich, Bein, Thomas

“…Chromobodies have recently drawn great attention as bioimaging nanotools. They offer high antigen binding specificity and affinity comparable to conventional…”
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Versatile and Efficient Site-Specific Protein Functionalization by Tubulin Tyrosine Ligase by Schumacher, Dominik, Helma, Jonas, Mann, Florian A., Pichler, Garwin, Natale, Francesco, Krause, Eberhard, Cardoso, M. Cristina, Hackenberger, Christian P. R., Leonhardt, Heinrich

“…A novel chemoenzymatic approach for simple and fast site‐specific protein labeling is reported. Recombinant tubulin tyrosine ligase (TTL) was repurposed to…”
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