Expression of osteotropic growth factors and growth hormone receptor in a canine distraction osteogenesis model

Osteotropic growth factors play an important role in bone metabolism. Nevertheless, knowledge about their expression in relation to distraction osteogenesis remains limited. The aim of the present study was to determine the expression of growth hormone (GH), growth hormone receptor (GHR), insulin-li...

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Bibliographic Details
Published in:	Journal of bone and mineral metabolism Vol. 24; no. 4; pp. 266 - 273
Main Authors:	THEYSE, Lars F. H, OOSTERLAKEN-DIJKSTERHUIS, Marja A, VAN DOOM, Jaap, TERLOU, Maarten, MOL, Jan A, VOORHOUT, George, HAZEWINKEL, Hemlan A. W
Format:	Journal Article
Language:	English
Published:	Tokyo Springer 01-07-2006 Springer Nature B.V
Subjects:	Animals Biological and medical sciences Bone growth Bone Morphogenetic Protein 2 Bone morphogenetic proteins Bone Morphogenetic Proteins - metabolism Bone turnover Consolidation Diseases of the osteoarticular system Distraction osteogenesis Dogs Female Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Regulation Growth factors Growth hormone Growth hormones Growth Substances - metabolism Head and neck surgery. Maxillofacial surgery. Dental surgery. Orthodontics Insulin Insulin-Like Growth Factor Binding Protein 4 - metabolism Insulin-Like Growth Factor Binding Protein 6 - metabolism Insulin-like growth factor I Insulin-Like Growth Factor I - metabolism Insulin-like growth factor II Insulin-Like Growth Factor II - metabolism Insulin-like growth factor-binding protein 4 Insulin-like growth factor-binding protein 6 Insulin-like growth factors Male Maxillofacial surgery. Dental surgery. Orthodontics Medical sciences Models, Animal mRNA Osteogenesis Osteogenesis, Distraction Osteotomy Receptors, Somatotropin - metabolism Regeneration Skeleton and joints Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Transforming Growth Factor beta - metabolism Vertebrates: osteoarticular system, musculoskeletal system Fissipedia Carnivora IGF-I growth hormone receptor Rheumatology Somatotropin hormone real-time PCR Insulin like growth factor 1 BMP-2 distraction osteogenesis Polymerase chain reaction Vertebrata Growth factor receptor Mammalia Animal Distraction Models Molecular biology Dog Osteogenesis
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Summary:	Osteotropic growth factors play an important role in bone metabolism. Nevertheless, knowledge about their expression in relation to distraction osteogenesis remains limited. The aim of the present study was to determine the expression of growth hormone (GH), growth hormone receptor (GHR), insulin-like growth factor I (IGF-I), insulin-like growth factor II (IGF-II), and bone morphogenetic protein 2 (BMP-2) in distraction-induced bone regeneration. Expression of these factors was assessed during the consolidation phase, comparing distraction osteogenesis with osteotomy-induced bone formation. Real-time PCR was performed as a semiquantitative measurement of mRNA, and the relative expression levels of these factors were determined. In addition, plasma GH profiles and plasma concentrations of IGF-I, IGF-II, and insulin-like growth factor-binding protein 4 and -6 (IGFBP-4 and -6) were measured to assess their potential systemic role during bone formation. Expression of GHR, IGF-I, and BMP-2 had significantly increased in comparison with the expression of these factors in mature bone. Expression of GHR was significantly higher in distraction-induced bone regenerate than in osteotomy-induced bone. No significant differences were found for the expression of IGF-I and BMP-2 between distraction and osteotomy. Plasma concentrations of GH, IGF-I, IGF-II, IGFBP-4, and IGFBP-6 did not demonstrate any significant differences between treatment groups and controls. Upregulation of GHR expression in distraction osteogenesis may enhance sensitivity to endogenous systemic GH and thus promote consolidation of the regenerated bone. Changes in the systemic osteotropic growth factors GH, IGF-I, IGF-II, IGFBP-4, and IGFBP-6 do not seem to be of importance during distraction osteogenesis.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0914-8779 1435-5604
DOI:	10.1007/s00774-006-0683-2