C3- and T-cell-dependent adjuvant activity of in vivo formed immune complexes

The effects of polyclonal antibodies to mouse serum components on the primary humoral immune response of mice in vivo were studied. It was observed that rabbit IgG to complement component C3 and albumin and mouse IgG to C5, but also heat-aggregated non-immune rabbit IgG, enhanced the agglutinating a...

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Bibliographic Details
Published in:	Immunology Vol. 73; no. 3; pp. 264 - 270
Main Authors:	VAN DEN BERG, C. W, HAZENBERG, M. A. J, HOFHUIS, F. M. A, VAN ROOYEN, S. M, VAN DIJK, H
Format:	Journal Article
Language:	English
Published:	Oxford Blackwell 01-07-1991
Subjects:	Adjuvants, Immunologic Animals Antibody Formation Antigen-Antibody Complex - immunology Biological and medical sciences Complement C3 - immunology Complement C5 - immunology Dose-Response Relationship, Immunologic Erythrocytes - immunology Female Fundamental and applied biological sciences. Psychology Fundamental immunology Immunobiology Immunoglobulin G - administration & dosage Immunoglobulin G - immunology Mice Mice, Inbred BALB C Mice, Inbred DBA Mice, Inbred Strains Modulation of the immune response (stimulation, suppression) Rabbits T-Lymphocytes - immunology Immunization Immune response Antibody Immunomodulation Complement C3 Rodentia Vertebrata Mammalia Mouse Immunological adjuvant T-Lymphocyte Humoral immunity Immune complex
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Summary:	The effects of polyclonal antibodies to mouse serum components on the primary humoral immune response of mice in vivo were studied. It was observed that rabbit IgG to complement component C3 and albumin and mouse IgG to C5, but also heat-aggregated non-immune rabbit IgG, enhanced the agglutinating antibody response to sheep erythrocytes (SRBC). Since the increase in response was only observed when antigen and antibodies were administered via the same route (i.p.), immunological adjuvant activity was implicated. Ineffectiveness of anti-C5 IgG in C5-deficient mice indicated that the antibody-induced adjuvant activity is mediated by in vivo formed immune complexes (IC). The adjuvant activity of IC was reduced by selective C3-depletion of animals, pointing to a requirement of C3. The effect of variations in other parameters was studied with anti-C3 and anti-C5 IgG as immunoadjuvant. The immunostimulatory effect was most pronounced when the antibodies were administered simultaneously with or shortly before antigen. Treatment of animals with antibodies one or two days before antigen, however, resulted in a suppression of the response. The response to thymus-independent antigens was not enhanced by anti-C3 nor by anti-C5 IgG. Optimal adjuvant activity of anti-C3 IgG was observed at low antigen doses. Nude mice were insensitive to the immunopotentiating effect of anti-C3 and so was the F1 progeny of BALB/c male and CBA/N female mice expressing a B-cell maturation defect. C5 deficiency and lipopolysaccharide (LPS) non-responsiveness did not affect the adjuvant activity of in vivo formed C3-anti-C3 IC.
ISSN:	0019-2805 1365-2567