Aerobic metabolism on muscle contraction in porcine iris sphincter

Aerobic metabolism on muscle contraction in porcine iris sphincter

Eyes are supplied O2 through the cornea and vessels of the retina and iris, which are tissues characterized by aerobic metabolism. Meanwhile, there are no reports on the association between iris sphincter contraction and aerobic metabolism. In this paper, we studied the aforementioned association. E...

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Bibliographic Details
Published in:Journal of Veterinary Medical Science Vol. 78; no. 11; pp. 1673 - 1676
Main Authors: KANDA, Hidenori, KANEDA, Takeharu, KATO, Asami, YOGO, Takuya, HARADA, Yasuji, HARA, Yasusi, URAKAWA, Norimoto, SHIMIZU, Kazumasa
Format: Journal Article
Language:English
Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 2016
Japan Science and Technology Agency
The Japanese Society of Veterinary Science
Subjects:
Abattoirs
Adenosine Triphosphate - metabolism
aerobic metabolism
Animals
ATP
Carbachol
Carbachol - pharmacology
Cell Hypoxia
Cornea
Creatine
Cyanides
Female
High-performance liquid chromatography
Hypoxia
Iris
Iris - drug effects
Iris - metabolism
iris sphincter
Male
Metabolism
Muscle contraction
Muscle Contraction - drug effects
Muscle Contraction - physiology
Muscle, Smooth - drug effects
Muscle, Smooth - metabolism
Oxidative metabolism
Oxygen consumption
Oxygen Consumption - physiology
Oxygen tension
Pharmacology
Phosphocreatine
Phosphocreatine - metabolism
porcine
Potassium chloride
Potassium Chloride - pharmacology
Sodium cyanide
Sodium Cyanide - pharmacology
Sphincter
Swine
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Summary:Eyes are supplied O2 through the cornea and vessels of the retina and iris, which are tissues characterized by aerobic metabolism. Meanwhile, there are no reports on the association between iris sphincter contraction and aerobic metabolism. In this paper, we studied the aforementioned association. Eyes from adult pigs of either sex were obtained from a local abattoir. A muscle strip was connected to a transducer to isometrically record the tension. O2 consumption was measured using a Clark-type polarograph connected to a biological oxygen monitor. Creatine phosphate (PCr) and adenosine triphosphate (ATP) contents were measured in the muscle strips by high-performance liquid chromatography (HPLC). Iris sphincter muscles were measured in resting, contractile or hypoxic phases. Contraction was induced by hyperosmotic 65 mM KCl (H-65K+) or carbachol (CCh), and hypoxia was induced by aeration with N2 instead of O2 or by addition of sodium cyanide (NaCN). H-65K+- and CCh-induced muscle contraction, involved increasing O2 consumption. Hypoxia and NaCN significantly decreased H-65K+- and CCh-induced muscle contraction and/or O2 consumption and PCr contents. Our results suggest that the contractile behavior in porcine iris sphincter highly depends on mitogen oxidative metabolism.
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ISSN:0916-7250
1347-7439
DOI:10.1292/jvms.16-0175