Antioxidant Activity of Tocotrienol Rich Fraction Prevents Fenitrothion-induced Renal Damage in Rats

Fenitrothion (FNT) is an organophosphate compound widely used as pesticide in Malaysia. The present study aims to investigate effects of palm oil tocotrienol rich fraction (TRF) on the renal damage of FNT-treated rats. A total of 40 male Sprague Dawley rats were divided into 4 groups randomly, the c...

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Bibliographic Details
Published in:	Journal of Toxicologic Pathology Vol. 26; no. 2; pp. 111 - 118
Main Authors:	BUDIN, Siti Balkis, HAN, Kim Jit, JAYUSMAN, Putri Ayu, TAIB, Izatus Shima, GHAZALI, Ahmad Rohi, MOHAMED, Jamaludin
Format:	Journal Article
Language:	English
Published:	Japan JAPANESE SOCIETY OF TOXICOLOGIC PATHOLOGY 2013 The Japanese Society of Toxicologic Pathology Japan Science and Technology Agency Japanese Society of Toxicologic Pathology
Subjects:	antioxidants histology lipid peroxidation organophosphate Original renal damage organophosphate histology antioxidants lipid peroxidation renal damage
Online Access:	Get full text
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Summary:	Fenitrothion (FNT) is an organophosphate compound widely used as pesticide in Malaysia. The present study aims to investigate effects of palm oil tocotrienol rich fraction (TRF) on the renal damage of FNT-treated rats. A total of 40 male Sprague Dawley rats were divided into 4 groups randomly, the control, TRF, FNT and FNT+TRF groups. FNT (20 mg/kg b.w.) and TRF (200 mg/kg b.w.) were given orally for 28 days continuously. Rats from the FNT+TRF group were supplemented with TRF 30 minutes prior to administration of FNT. Rats were sacrificed after 28 days, and the kidneys were removed for determination of oxidative stress and histological analysis. Plasma was collected for determination of blood creatinine and urea level. Statistical analysis showed that palm oil TRF has a protective effect against renal oxidative damage induced by FNT. In the FNT+TRF group, malondialdehyde and protein carbonyl levels were significantly lower, while the glutathione level as well as superoxide dismutase and catalase activities were significantly higher compared with the FNT-treated group (p<0.05). As for renal function, there was a markedly lower urea level (p<0.05) in the FNT+TRF group compared with the FNT-treated group, but there was no significant difference in creatinine level. Besides, total protein also showed no significant difference for all groups of rats (p>0.05). Histological evaluation also revealed that the FNT+TRF group had less glomerulus and renal tubule damage than the FNT-treated group. In conclusion, palm oil TRF was able to reduce oxidative stress and renal damage in FNT-treated rats.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0914-9198 1881-915X 1347-7404
DOI:	10.1293/tox.26.111