Search Results - "Härmä, Harri"

Homogeneous Dual-Parametric-Coupled Assay for Simultaneous Nucleotide Exchange and KRAS/RAF-RBD Interaction Monitoring by Kopra, Kari, Vuorinen, Emmiliisa, Abreu-Blanco, Maria, Wang, Qi, Eskonen, Ville, Gillette, William, Pulliainen, Arto T, Holderfield, Matthew, Härmä, Harri

“…We have developed a rapid and sensitive single-well dual-parametric method introduced in linked RAS nucleotide exchange and RAS/RAF-RBD interaction assays. RAS…”
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Sensitive Label-Free Thermal Stability Assay for Protein Denaturation and Protein–Ligand Interaction Studies by Vuorinen, Emmiliisa, Valtonen, Salla, Eskonen, Ville, Kariniemi, Taru, Jakovleva, Jelena, Kopra, Kari, Härmä, Harri

“…In modern biochemistry, protein stability and ligand interactions are of high interest. These properties are often studied with methods requiring labeled…”
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Nanomolar Protein–Protein Interaction Monitoring with a Label-Free Protein-Probe Technique by Valtonen, Salla, Vuorinen, Emmiliisa, Kariniemi, Taru, Eskonen, Ville, Le Quesne, John, Bushell, Martin, Härmä, Harri, Kopra, Kari

“…Protein–protein interactions (PPIs) are an essential part of correct cellular functionality, making them increasingly interesting drug targets. While Förster…”
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Isothermal chemical denaturation assay for monitoring protein stability and inhibitor interactions by Mahran, Randa, Vello, Niklas, Komulainen, Anita, Malakoutikhah, Morteza, Härmä, Harri, Kopra, Kari

“…Thermal shift assay (TSA) with altered temperature has been the most widely used method for monitoring protein stability for drug research. However, there is a…”
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Nanomolar Protein Thermal Profiling with Modified Cyanine Dyes by Malakoutikhah, Morteza, Mahran, Randa, Gooran, Negin, Masoumi, Ahmadreza, Lundell, Katri, Liljeblad, Arto, Guiley, Keelan, Dai, Shizhong, Zheng, Qinheng, Zhu, Lawrence, Shokat, Kevan M., Kopra, Kari, Härmä, Harri

“…Protein properties and interactions have been widely investigated by using external labels. However, the micromolar sensitivity of the current dyes limits…”
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Homogeneous single-label cGMP detection platform for the functional study of nitric oxide-sensitive (soluble) guanylyl cyclases and cGMP-specific phosphodiesterases by Kopra, Kari, Sharina, Iraida, Martin, Emil, Härmä, Harri

“…Cardiovascular diseases are the number one death worldwide. Nitric oxide (NO)—NO-sensitive (soluble) guanylyl cyclase (sGC)—cyclic guanosine monophosphate…”
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Single-Peptide TR-FRET Detection Platform for Cysteine-Specific Post-Translational Modifications by Eskonen, Ville, Tong-Ochoa, Natalia, Mattsson, Leena, Miettinen, Moona, Lastusaari, Mika, Pulliainen, Arto T, Kopra, Kari, Härmä, Harri

“…Post-translational modifications (PTMs) are one of the most important regulatory mechanisms in cells, and they play key roles in cell signaling both in health…”
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Beyond KRAS(G12C): Biochemical and Computational Characterization of Sotorasib and Adagrasib Binding Specificity and the Critical Role of H95 and Y96 by Mahran, Randa, Kapp, Jonas N., Valtonen, Salla, Champagne, Allison, Ning, Jinying, Gillette, William, Stephen, Andrew G., Hao, Feng, Plückthun, Andreas, Härmä, Harri, Pantsar, Tatu, Kopra, Kari

“…Mutated KRAS proteins are frequently expressed in some of the most lethal human cancers and thus have been a target of intensive drug discovery efforts for…”
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Quenching resonance energy transfer (QRET): a single-label technique for inhibitor screening and interaction studies by Kopra, Kari, Härmä, Harri

“…•Homogeneous QRET technology is applicable for wide range of different targets.•The technology relies on weak interaction between lanthanide chelate and…”
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Method for Estimation of Protein Isoelectric Point by Pihlasalo, Sari, Auranen, Laura, Hänninen, Pekka, Härmä, Harri

“…Adsorption of sample protein to Eu3+ chelate-labeled nanoparticles is the basis of the developed noncompetitive and homogeneous method for the estimation of…”
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Immunoassay of C-reactive protein by hot electron-induced electrochemiluminescence at polystyrene-carbon black composite electrodes by Salminen, Kalle, Grönroos, Päivi, Eskola, Jarkko, Nieminen, Eemi, Härmä, Harri, Kulmala, Sakari

“…Aromatic Tb(III) chelates can be detected down to subnanomolar concentrations at polystyrene-carbon black composite electrodes on the basis of hot…”
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Hot electron-induced electrochemiluminescence of calcein and calcein-Tb(III) complex at disposable oxide-covered aluminum and polyvinyl butyral-carbon black/metal composite electrodes in aqueous solutions by Salminen, Kalle, Grönroos, Päivi, Härmä, Harri, Kulmala, Sakari

“…Hot electron-induced electrochemiluminescence (HECL) of calcein and calcein-Tb(III) complex was generated at oxide-covered aluminum electrode during cathodic…”
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High-throughput amenable fluorescence-assays to screen for calmodulin-inhibitors by Manoharan, Ganesh babu, Kopra, Kari, Eskonen, Ville, Härmä, Harri, Abankwa, Daniel

“…The KRAS gene is highly mutated in human cancers and the focus of current Ras drug development efforts. Recently the interface between the C-terminus of K-Ras…”
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Lanthanide Label Array Method for Identification and Adulteration of Honey and Cacao by Härmä, Harri, Peltomaa, Riikka, Pihlasalo, Sari

“…A generic, cost-effective, and simple method has been developed to fingerprint liquids to differentiate food brands and ingredients. The method is based on a…”
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QTR-FRET: Efficient background reduction technology in time-resolved förster resonance energy transfer assays by Syrjänpää, Markku, Vuorinen, Emmiliisa, Kulmala, Sakari, Wang, Qi, Härmä, Harri, Kopra, Kari

“…A novel homogeneous assay system QTR-FRET (Quencher modulated Time-Resolved Förster Resonance Energy Transfer) combining quenching resonance energy transfer…”
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Protease Substrate-Independent Universal Assay for Monitoring Digestion of Native Unmodified Proteins by Vuorinen, Emmiliisa, Valtonen, Salla, Hassan, Nazia, Mahran, Randa, Habib, Huda, Malakoutikhah, Morteza, Kopra, Kari, Härmä, Harri

“…Proteases are a group of enzymes with a catalytic function to hydrolyze peptide bonds of proteins. Proteases regulate the activity, signaling mechanism, fate,…”
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Sensitive, homogeneous, and label-free protein-probe assay for antibody aggregation and thermal stability studies by Valtonen, Salla, Vuorinen, Emmiliisa, Eskonen, Ville, Malakoutikhah, Morteza, Kopra, Kari, Härmä, Harri

“…Protein aggregation is a spontaneous process affected by multiple external and internal properties, such as buffer composition and storage temperature…”
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Luminophore Chemistry for Detection of Urinary Bladder Cancer – Comparison to Cytology and Urinary Rapid Tests (BTA stat ® , NMP22 ® BladderChek ® and UBC ® Rapid Test) by ECKE, THORSTEN H., SCISLOWSKI, MIKOLAJ, HASSAN, NAZIA, SAURA, MARI, HALLMANN, STEFFEN, KOCH, STEFAN, VUOLLE, SARI, MALAKOUTIKHAH, MORTEZA, KOPRA, KARI, HÄRMÄ, HARRI

“…Background/Aim: New luminometric chelates were developed for the detection of urinary bladder cancer and compared to cytology and urinary rapid tests BTA…”
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Luminometric Label Array for Quantification and Identification of Metal Ions by Pihlasalo, Sari, Montoya Perez, Ileana, Hollo, Niklas, Hokkanen, Elina, Pahikkala, Tapio, Härmä, Harri

“…Quantification and identification of metal ions has gained interest in drinking water and environmental analyses. We have developed a novel label array method…”
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Rapid high-throughput compatible label-free virus particle quantification method based on time-resolved luminescence by Kopra, Kari, Hassan, Nazia, Vuorinen, Emmiliisa, Valtonen, Salla, Mahran, Randa, Habib, Huda, Jalkanen, Pinja, Susi, Petri, Hytönen, Vesa, Hankaniemi, Minna, Ylä-Herttuala, Seppo, Kakkola, Laura, Peurla, Markus, Härmä, Harri

“…Viruses play a major role in modern society and create risks from global pandemics and bioterrorism to challenges in agriculture. Virus infectivity assays and…”
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