Beta-7 integrin controls enterocyte migration in the small intestine

Beta-7 integrin controls enterocyte migration in the small intestine

AIM:To hypothesize that beta-7 integrin affects cellularmigration of both,lymphocytes and enterocytes.METHODS:The nucleoside analog Brd U was ip injected in beta-7-deficient mice(C57BL/6-Itgbtmlcgn/J)of male gender and age-matched male C57BL/J J mice(wild type)4,20,or 40 h before analysis.The total...

Full description

Saved in:
Bibliographic Details
Published in:World journal of gastroenterology : WJG Vol. 21; no. 6; pp. 1759 - 1764
Main Authors: Kaemmerer, Elke, Kuhn, Paula, Schneider, Ursula, Clahsen, Thomas, Jeon, Min Kyung, Klaus, Christina, Andruszkow, Julia, Härer, Michael, Ernst, Sabine, Schippers, Angela, Wagner, Norbert, Gassler, Nikolaus
Format: Journal Article
Language:English
Published: United States Baishideng Publishing Group Inc 14-02-2015
Subjects:
Animals
Barrier
Basic Study
bowel
Cell Movement
Enterocytes - metabolism
function;Cell
Integrin beta Chains - genetics
Integrin beta Chains - metabolism
Intestinal Mucosa - cytology
Intestinal Mucosa - metabolism
Intestine, Small - cytology
Intestine, Small - metabolism
Lymphocytes - metabolism
Male
Mice, Inbred C57BL
Mice, Knockout
migration;Inflammatory
Signal Transduction
Time Factors
Inflammatory bowel disease
Integrin
Intercellular junctions
Barrier function
Cell migration
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:AIM:To hypothesize that beta-7 integrin affects cellularmigration of both,lymphocytes and enterocytes.METHODS:The nucleoside analog Brd U was ip injected in beta-7-deficient mice(C57BL/6-Itgbtmlcgn/J)of male gender and age-matched male C57BL/J J mice(wild type)4,20,or 40 h before analysis.The total small intestine was isolated,dissected,and used for morphometrical studies.Brd U-positive epithelial cells were numbered in at least 15 hemi-crypts per duodenum,jejunum,and ileum of each animal.The outer most Brd U-positive cell(cellmax)was determined per hemi-crypt,numerically documented,and statistically analysed.RESULTS:Integrins containing the beta-7-chain were exclusively expressed on leukocytes.In the small intestinal mucosa of beta-7 integrin-deficient mice the number of intraepithelial lymphocytes was drastically decreased.Moreover,the Peyer’s patches of beta-7integrin-deficient mice appeared hypoplastic.In beta-7integrin-deficient mice the location of cellmax was found in a higher position than it was the case for the controls.The difference was already detected at 4 h after Brd U application,but significantly increased with time(40 h after Brd U injection)in all small intestinal segments investigated,i.e.,duodenum,jejunum,and ileum.Migration of small intestinal enterocytes was different between the experimental groups measured by cellmax locations.CONCLUSION:The E-cadherin beta-7 integrin pathway probably controls migration of enterocytes within the small intestinal surface lining epithelial layer.
Bibliography:AIM:To hypothesize that beta-7 integrin affects cellularmigration of both,lymphocytes and enterocytes.METHODS:The nucleoside analog Brd U was ip injected in beta-7-deficient mice(C57BL/6-Itgbtmlcgn/J)of male gender and age-matched male C57BL/J J mice(wild type)4,20,or 40 h before analysis.The total small intestine was isolated,dissected,and used for morphometrical studies.Brd U-positive epithelial cells were numbered in at least 15 hemi-crypts per duodenum,jejunum,and ileum of each animal.The outer most Brd U-positive cell(cellmax)was determined per hemi-crypt,numerically documented,and statistically analysed.RESULTS:Integrins containing the beta-7-chain were exclusively expressed on leukocytes.In the small intestinal mucosa of beta-7 integrin-deficient mice the number of intraepithelial lymphocytes was drastically decreased.Moreover,the Peyer’s patches of beta-7integrin-deficient mice appeared hypoplastic.In beta-7integrin-deficient mice the location of cellmax was found in a higher position than it was the case for the controls.The difference was already detected at 4 h after Brd U application,but significantly increased with time(40 h after Brd U injection)in all small intestinal segments investigated,i.e.,duodenum,jejunum,and ileum.Migration of small intestinal enterocytes was different between the experimental groups measured by cellmax locations.CONCLUSION:The E-cadherin beta-7 integrin pathway probably controls migration of enterocytes within the small intestinal surface lining epithelial layer.
Barrier function;Cell migration;Inflammatory bowel
Elke Kaemmerer;Paula Kuhn;Ursula Schneider;Thomas Clahsen;Min Kyung Jeon;Christina Klaus;Julia Andruszkow;Michael Hrer;Sabine Ernst;Angela Schippers;Norbert Wagner;Nikolaus Gassler;Institute of Pathology, RWTH Aachen University;Department of Pediatrics, RWTH Aachen University;Department of Medical Statistics, RWTH Aachen University
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Correspondence to: Nikolaus Gassler, Professor, Institute of Pathology, RWTH Aachen University, Pauwelsstraße 30, 52074 Aachen, Germany. ngassler@ukaachen.de
Telephone: +49-241-8088897 Fax: +49-241-8082439
Author contributions: Kaemmerer E, Klaus C and Gassler N designed the research; Kaemmerer E, Kuhn P, Schneider U, Clahsen T and Jeon M performed the research; Andruszkow J, Härer M and Ernst S contributed analytic tools; Kaemmerer E, Clahsen T, Klaus C, Schippers A, Wagner N and Gassler N wrote the paper; Kaemmerer E and Kuhn P contributed equally.
ISSN:1007-9327
2219-2840
DOI:10.3748/wjg.v21.i6.1759